Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

A 6 D.O.F. opto-inertial tracker for virtual reality experiments in microgravity.

Gravity plays a role in many different levels of human motor behavior. It dictates the laws of motion of our body and limbs, as well as of the objects in the external world with which we wish to interact. The dynamic interaction of our body with the world is molded within gravity's constraints. The role played by gravity in the perception of visual stimuli and the elaboration of human movement is an active research theme in the field of Neurophysiology. Conditions of microgravity, coupled with techniques from the world of virtual reality, provide a unique opportunity to address these questions concerning the function of the human sensorimotor system. The ability to measure movements of the head and to update in real time the visual scene presented to the subject based on these measurements is a key element in producing a realistic virtual environment. A variety of head-tracking hardware exists on the market today, but none seem particularly well suited to the constraints of working with a space station environment. Nor can any of the existing commercial systems meet the more stringent requirements for physiological experimentation (high accuracy, high resolution, low jitter, low lag) in a wireless configuration. To this end, we have developed and tested a hybrid opto-inertial 6 degree-of-freedom tracker based on existing inertial technology. To confirm that the inertial components and algorithms will function properly, this system was tested in the microgravity conditions of parabolic flight. Here we present the design goals of this tracker, the system configuration and the results of 0g and 1g testing.     

Neuritogenesis: a model for space radiation effects on the central nervous system.

Pivotal to the astronauts' functional integrity and survival during long space flights are the strategies to deal with space radiations. The majority of the cellular studies in this area emphasize simple endpoints such as growth related events which, although useful to understand the nature of primary cell injury, have poor predictive value for extrapolation to more complex tissues such as the central nervous system (CNS). In order to assess the radiation damage on neural cell populations, we developed an in vitro model in which neuronal differentiation, neurite extension, and synaptogenesis occur under controlled conditions. The model exploits chick embryo neural explants to study the effects of radiations on neuritogenesis. In addition, neurobiological problems associated with long-term space flights are discussed.     

Relaxation of electron and proton radiation belts of the earth after strong magnetic storms

During strong magnetic storms in July and November of 2004 the fluxes of trapped particles (protons and electrons of MeV energies) in the Earth’s radiation belts have increased by orders of magnitude and then decreased remaining on an enhanced level for several months. These enhancements allowed us to study the processes of relaxation of the radiation belts. Measurements of energetic particles by low-altitude satellites Coronas-F and Servis-1 have shown that predictions of the theory about the rate of pitch-angle diffusion are not always correct, giving both overestimated and underestimated values for the lifetime of energetic particles.     

Summary of radiation dosimetry results on U.S. and Soviet manned spacecraft.

Measurements of the radiation environment aboard U.S. and Soviet manned spacecraft are reviewed and summarized. Data obtained mostly from passive and some active radiation detectors now exist for the case of low Earth-orbit missions. Major uncertainties still exist for space exposure in high altitude, high inclination, geostationary orbits, in connection with solar effects and that of shielding. Data from active detectors flown in Spacelabs 1 and 2 suggest that a variety of phenomena must be understood before the effects of long-term exposure at the space-station type of orbit and shielding can be properly assessed.     

Adaptive Array Bandwidth with Tapped Delay-Line Processing

The bandwidth of adaptive arrays with tapped delay lines behind the elements is examined. Such processing offers improved bandw over that attainable with quadrature hybrid processing. The performance of a two-element array with four types of processing (equarature hybrids, single delay lines, 3-tap delay lines, and 5-tap delay lines) is compared. It is shown that with half-wavelength element spacing, a quadrature hybrid and single delay-line processor are inadequate at 10-percent bandwidth. A 3-tap processor is adeq however, up to 40-percent bandwidth.     

Satellite observations of upper atmosphere O3 and HNO3 from the Limb Infrared Monitor of the Stratosphere (LIMS) experiment on Nimbus 7

The Limb Infrared Monitor of the Stratosphere (LIMS) experiment is a limb scanning infrared sounder designed to measure vertical temperature profiles and the concentrations of key chemical compounds which are important in the stratospheric ozone-nitrogen photochemistry. This paper describes results from the O₃ and HNO₃ channels with emphasis on validation of the data. Similar discussions of results from the other channels are presented in two companion papers published in these proceedings.     

Cygnus X-1 revisited

We review the X- and gamma-ray observations of Cygnus X-1 and their theoretical interpretations, with emphasis on new developments since the mid-1970's. The overall data base at present is most consistent with the inverse Compton model by hot thermal electrons of T _e 10⁹ K, for the hard X-ray luminosity (10–200 keV). However, the origin of the soft X-rays ( 10 keV) in high states and gamma rays (> 200 keV) remain unsettled.Operated under DOE Contract W-7405-Eng-48.Partially supported by NASA Grant NGR 05-020-668.NRC/NRL Research Associate.     

FIGARO: An experiment for pulsar and variable source studies in the MeV range

We present a large area, balloon borne, NaI(Tl) detector for low-energy gamma rays with temporal signature : FIGARO.The main detector is a mosaic of 12 NaI(Tl) tiles 22.5 × 15 × 5 cm, for a total geometric area of 4050 cm².In the energy band 140 keV - 6 MeV, the expected background counting rate at float altitude is in the range of two to three thousands counts per second.For pulsar analysis the expected 3δ sensitivity for 5 hours exposition time is 2.5 10^?4 ph/cm².s.MeV (150–500 keV) 1.5 10^?4 ph/cm².s.MeV (1–6 MeV). This performance, together with the large effective area and the relatively short duration of a balloon flight, make FIGARO particularly suitable for the identification of sources by means of temporal analysis.For objectives in the Northern sky, including the Crab pulsar, a transmediterranean flight is planned for the summer of 1982 ; a Southern mission is scheduled in Brazil for the fall of 1983 (Vela, PSR 1822-09).