Using a mini-Raman spectrometer to monitor the adaptive strategies of extremophile colonizers in arid deserts: relationships between signal strength, adaptive strategies, solar radiation, and humidity

Abstract The survival strategies of one cyanobacteria colony and three terricolous lichen species from the hot subdesert of Tabernas, Spain, were studied along with topographical attributes of the area to investigate whether the protective strategies adopted by these pioneer soil colonizers are related to the environmental stressors under which they survive. A handheld Raman spectrometer was used for biomolecular characterization, while the microclimatic and topographic parameters were estimated with a Geographic Information System (GIS). We found that the survival strategies adopted by those organisms are based on different combinations of protective biomolecules, each with diverse ecophysiological functions, such as UV-radiation screening, free-energy quenching, antioxidants, and the production of different types and amounts of calcium oxalates. Our results show that the cyanobacteria community and each lichen species preferentially colonized a particular microhabitat with specific moisture and incident solar radiation levels and exhibited different adaptive mechanisms. In recent years, a number of studies have provided consistent results that suggest a link between the strategies adopted by those extremophile organisms and the microclimatic environmental parameters. To date, however, far too little attention has been paid to results from Raman analyses on dry specimens. Therefore, the results of the present study, produced with the use of our miniaturized instrument, will be of interest to future studies in astrobiology, especially due to the likely use of Raman spectroscopy at the surface of Mars. Key Words: Hot desert-Raman spectroscopy-Topography-Terricolous lichens-Cyanobacteria-Planetary exploration. Astrobiology 12, 743-753.     

The impact of large solar events on the total electron content of the ionosphere at mid latitudes

Ionospheric disturbances associated with solar activity may occur via two basic mechanisms. The first is related to the direct impact on the ionosphere of EUV photons from a flare, and the second by prompt electric field penetration into the magnetosphere during geomagnetic storms. In this paper we examine the possibility that these two mechanisms may have an impact at mid latitudes by calculating the total electron content (TEC) from GPS stations in Mexico during several large X-ray flares. We have found that indeed large, complex flares, which are well located, may affect the mid latitude ionosphere. In fact, in the solar events of July 14, 2000 and April 2001 storms, ionospheric disturbances were observed to increase up to 138 and 150 TECu, respectively, due to the influence of EUV photons. Also, during the solar events of July 2000, April 2001, Halloween 2003, January 2005 and December 2006, there are large ionospheric disturbances (up to 393 TECu in the Halloween Storms), due to prompt penetration electric field, associated with CME producing geomagnetic storm.     

Capacity building in space law and space policy

A high level of expertise in space policy and law is required to initiate and keep pace with the expansion of space activities, including those undertaken by the private space industry. Space activities generally refer to those undertakings that are carried out with the use of several technologies for the exploration and utilization of outer space often for scientific, military, economic and social proposes within international and national policy and regulatory frameworks. Space policies and legal regimes determine the scope, nature, pace, possibility and development of space undertakings. Therefore, appropriate space policies and regulatory regimes, both at the international and national levels, are indispensable for the initiation, operation and enhancement of space activities. There are various regulatory models and approaches for regulating space activities, and for building capacity in space law and space policy. It is imperative to have the appropriate human resources and capabilities in the development and implementation of space policies and space legal regimes. This crucial requirement has been well recognized in the more advanced space-faring nations, but not to the same level in other space-faring or space-aspiring nations.     

Laboratory calibration measurements of a piezoelectric lead zirconate titanate cosmic dust detector at low velocities

A cosmic dust monitor for use onboard a spacecraft is currently being developed using a piezoelectric lead zirconate titanate element (PZT). Its characteristics of the PZT sensor is studied by ground-based laboratory impact experiments using hypervelocity particles supplied by a Van de Graaff accelerator. The output signals obtained from the sensor just after the impact appeared to have a waveform that was explicitly related to the particle’s impact velocity. For velocities less than ∼6 km/s, the signal showed an oscillation pattern and the amplitude was proportional to the momentum of the impacting particle. For higher velocities, the signal gradually changed to a single waveform. The rise time of this single waveform was proportional to the particle’s velocity for velocities above ∼6 km/s. The present paper reports on results for the low velocity case and especially discusses the effect of an outer coating of the sensor with a paint, which is used to reduce heating by solar radiation.     

The case for enhanced India–Canada space cooperation

Based on the premise that both India and Canada are spacefaring nations actively involved in the exploration and use of outer space towards scientific and commercial ends, this paper seeks to pave the way for each of the two countries to derive optimum scientific and economic benefits from their space activities through bilateral cooperation. Although each country's space programme has a different focus, the analysis in the paper demonstrates that they could be highly complementary of, and beneficial to, each other. Notwithstanding the presently conducive political environment for bilateral cooperation, as evidenced by the recent high-level political exchanges between the two countries, there are several regulatory and policy obstacles that will hinder any efforts towards bilateral space cooperation. The paper proffers some viable options for overcoming the challenges identified or envisaged.     

Ricardo Dyrgalla (1910–1970), pioneer of rocket development in Argentina

One of the most important developers of liquid propellant rocket engines in Argentina was Polish-born Ricardo Dyrgalla. Dyrgalla immigrated to Argentina from the United Kingdom in 1946, where he had been studying German weapons development at the end of the Second World War. A trained pilot and aeronautical engineer, he understood the intricacies of rocket propulsion and was eager to find practical applications to his recently gained knowledge.Dyrgalla arrived in Argentina during Juan Perón's first presidency, a time when technicians from all over Europe were being recruited to work in various projects for the recently created Argentine Air Force.Shortly after immigrating, Dyrgalla proposed to develop an advanced air-launched weapon, the Tábano, based on a rocket engine of his design, the AN-1. After a successful development program, the Tábano was tested between 1949 and 1951; however, the project was canceled by the government shortly after. Today, the AN-1 rocket engine is recognized as the first liquid propellant rocket to be developed in South America. Besides the AN-1, Dyrgalla also developed several other rockets systems in Argentina, including the PROSON, a solid-propellant rocket launcher developed by the Argentine Institute of Science and Technology for the Armed Forces (CITEFA). In the late 1960s, Dyrgalla and his family relocated to Brazil due mostly to the lack of continuation of rocket development in Argentina. There, he worked for the Institute of Aerospace Technology (ITA) until his untimely death in 1970. Ricardo Dyrgalla deserves to be recognized among the world's rocket pioneers and his contribution to the science and engineering of rocketry deserves a special place in the history of South America's rocketry and space flight advocacy programs.     

Instrument study of the Lunar Dust eXplorer (LDX) for a lunar lander mission

One of the highest-priority issues for a future human or robotic lunar exploration is the lunar dust. This problem should be studied in depth in order to develop an environment model for a future lunar exploration. A future ESA lunar lander mission requires the measurement of dust transport phenomena above the lunar surface. Here, we describe an instrument design concept to measure slow and fast moving charged lunar dust which is based on the principle of charge induction. LDX has a low mass and measures the speed and trajectory of individual dust particles with sizes below one micrometer. Furthermore, LDX has an impact ionization target to monitor the interplanetary dust background. The sensor consists of three planes of segmented grid electrodes and each electrode is connected to an individual charge sensitive amplifier. Numerical signals were computed using the Coulomb software package. The LDX sensitive area is approximately 400 cm². Our simulations reveal trajectory uncertainties of better than 2° with an absolute position accuracy of better than 2 mm.     

Rare observation of daytime whistlers at very low latitude (L = 1.08)

The source region and propagation mechanism of low latitude whistlers (Geomag. lat. <30°) have puzzled scientific community for last many decades. In view of recent reports, there is consensus on the source region of low latitude whistlers in the vicinity of the conjugate point. But the plausible conditions of ionospheric medium through which they travel are still uncertain. In addition to that, the whistlers in daytime are never observed at geomagnetic latitudes less than 20°. Here, for the first time, we present a rare observations of whistlers during sunlit hours from a very low-latitude station Allahabad (Geomag. Lat: 16.79°N, L = 1.08) in India on 04 February 2011. More than 90 whistlers are recorded during 1200–1300 UT during which the whole propagation path from lightning source region to whistler observation site is under sunlit. The favorable factors that facilitated the whistlers prior to the sunset are investigated in terms of source lightning characteristics, geomagnetic and background ionospheric medium conditions. The whistler activity period was found to be geomagnetically quiet. However, a significant suppression in ionospheric total electron content (TEC) compared to its quiet day average is found. This shows that background ionospheric conditions may play a key role in low latitude whistler propagation. This study reveals that whistlers can occur under sunlit hours at latitudes as low as L = 1.08 when the source lightning and ionospheric medium characteristics are optimally favorable.     

Potential of cathodoluminescence microscopy and spectroscopy for the detection of prokaryotic cells on minerals

Detecting mineral-hosted ecosystems to assess the extent and functioning of the biosphere from the surface to deep Earth requires appropriate techniques that provide, beyond the morphological criteria, indubitable clues of the presence of prokaryotic cells. Here, we evaluate the capability of cathodoluminescence microscopy and spectroscopy, implemented on a scanning electron microscope, to identify prokaryotes on mineral surfaces. For this purpose, we used, as a first step, a simple model of either unstained or stained cultivable cells (Escherichia coli, Deinococcus radiodurans) deposited on minerals that are common in the oceanic crust (basaltic glass, amphibole, pyroxene, and magnetite). Our results demonstrate that the detection of cells is possible at the micrometric level on the investigated minerals through the intrinsic fluorescence of their constituting macromolecules (aromatic amino and nucleic acids, coenzymes). This allows us to distinguish biomorph inorganic phases from cells. This easily implemented technique permits an exploration of colonized rock samples. In addition, the range of spectrometric techniques available on a scanning electron microscope can provide additional information on the nature and chemistry of the associated mineral phases, which would lead to a simultaneous characterization of cells, their microhabitats, and a better understanding of their potential relationships.