Extreme dryness and DNA-protein cross-links.

Exposure of fungal conidia (Aspergillus ochraceus) or spores of Bacillus subtilis to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered water activity results in an increasing number of cross-links between DNA and proteins. In fungal conidia these cross-links are detected after selective iodination (125 J) of the DNA-bound proteins followed by gel electrophoresis and subsequent autoradiography. Another approach is the labelling of DNA with 32P by means of nick translation and the detection of differences in the electrophoretic mobility of DNA before and after digestion with proteinase K of proteins bound to DNA.     

Infrared spectroscopy of organics of planetological interest at low temperatures.

In the context of prebiotic chemistry in space, some of the outer planetary objects display H, C, N and O rich chemistry similar to the one in the biosphere of Earth. Of particular interest are Saturn's moon, Titan; Neptune's moon, Triton; and Pluto where extreme cold conditions prevail. Identifications of chemical species on these objects (surfaces and atmospheres) is essential to a better understanding of the radiation induced chemical reactions occurring thereon. There have been several ground based observations of these planetary objects in the infrared windows from 1 to 2.5 micrometers. Voyager also provided spectra in the thermal infrared (6 to 50 micrometers) region. Interpretation of these data require laboratory infrared spectra of relevant species under the temperature conditions appropriate to these objects. The results of some of these studies carried out in our laboratory and elsewhere and their impact on the analyses of the observed data will be summarized.     

Development of x-ray mirrors for high-energy astrophysics in czechoslovakia

The grazing incidence X-ray mirrors with diameters between 20 and 240 mm were produced by two different replica techniques. Five mirrors were flown in space experiments. It has been confirmed that the galvanoplastic replica mirrors are well suited for applications in space telescopes and laboratory microscopes.     

The statistical properties of coronal mass ejections during 1979–1981

The Solwind coronagraph on the P78-1 earth-orbiting satellite has been monitoring the Sun routinely at 10-minute intervals during the 5-year interval from April, 1979 to the present. In a statistical analysis of about 1000 mass ejections observed through the end of 1981, we find an average occurrence rate of 1.8 mass ejections per day. Histograms of speed, central latitude, angular span, brightness, and other parameters have been constructed, and properties such as shape classification have been tabulated. These characteristics are summarized for these years near sunspot maximum. The average speed and mass estimate are found to be similar to those found at the declining phase of the previous sunspot cycle. The angular span and central latitude distributions are quite different than seen during the declining phase, and are very dependent upon structural class. The fluctuations in the occurrence rate of CMEs does not seem to match the fluctuations in the sunspot number. There is a tendency for high speed CMEs to occur more frequently in 1981 than in 1980, and more frequently in 1980 than in 1979.     

Cellular parameters for track structure modeling of radiation hazard in space.

Based on irradiation with 45 MeV/u N and B ions and with Co-60 gamma rays, cellular parameters of Katz's track structure model have been fitted for the survival of V79-379A Chinese hamster lung fibroblasts. Cellular parameters representing neoplastic transformations in C3H10T/1/2 cells after their irradiation with heavy ion beams, taken from earlier work, were also used to model the radiation hazard in deep space, following the system for evaluating, summing and reporting occupational exposures proposed in 1967 by a subcommittee of NCRP. We have performed model calculations of the number of transformations in surviving cells, after a given fluence of heavy charged particles of initial energy 500 MeV/u, penetrating thick layers of cells. We take the product of cell transformation and survival probabilities, calculated along the path lengths of charged particles using cellular survival and transformation parameters, to represent a quantity proportional to the "radiation risk factor" discussed in the NCRP document. The "synergistic" effect of simultaneous charged particle transfers is accounted for by the "track overlap" mode inherent in the model of Katz.     

Recent NASA research accomplishments aboard the ISS

The activation of the US Laboratory Module "Destiny" on the International Space Station (ISS) in February 2001 launched a new era in microgravity research. Destiny provides the environment to conduct long-term microgravity research utilizing human intervention to assess, report, and modify experiments real time. As the only available pressurized space platform, ISS maximizes today's scientific resources and substantially increases the opportunity to obtain much longed-for answers on the effects of microgravity and long-term exposure to space. In addition, it evokes unexpected questions and results while experiments are still being conducted, affording time for changes and further investigation. While building and outfitting the ISS is the main priority during the current ISS assembly phase, seven different space station crews have already spent more than 2000 crew hours on approximately 80 scientific investigations, technology development activities, and educational demonstrations.     

Regolith X-Ray Imaging Spectrometer (REXIS) Aboard the OSIRIS-REx Asteroid Sample Return Mission

The Regolith X-ray Imaging Spectrometer (REXIS) is the student collaboration experiment proposed and built by an MIT-Harvard team, launched aboard NASA’s OSIRIS-REx asteroid sample return mission. REXIS complements the scientific investigations of other OSIRIS-REx instruments by determining the relative abundances of key elements present on the asteroid’s surface by measuring the X-ray fluorescence spectrum (stimulated by the natural solar X-ray flux) over the range of energies 0.5 to 7 keV. REXIS consists of two components: a main imaging spectrometer with a coded aperture mask and a separate solar X-ray monitor to account for the Sun’s variability. In addition to element abundance ratios (relative to Si) pinpointing the asteroid’s most likely meteorite association, REXIS also maps elemental abundance variability across the asteroid’s surface using the asteroid’s rotation as well as the spacecraft’s orbital motion. Image reconstruction at the highest resolution is facilitated by the coded aperture mask. Through this operation, REXIS will be the first application of X-ray coded aperture imaging to planetary surface mapping, making this student-built instrument a pathfinder toward future planetary exploration. To date, 60 students at the undergraduate and graduate levels have been involved with the REXIS project, with the hands-on experience translating to a dozen Master’s and Ph.D. theses and other student publications.     

Analysis of the dynamic behavior of structures using the high-rate GNSS-PPP method combined with a wavelet-neural model: Numerical simulation and experimental tests

Recently, the high rate global navigation satellite system-precise point positioning (GNSS-PPP) technique has been used to detect the dynamic behavior of structures. This study aimed to increase the accuracy of the extraction oscillation properties of structural movements based on the high-rate (10?Hz) GNSS-PPP monitoring technique. A developmental model based on the combination of wavelet package transformation (WPT) de-noising and neural network prediction (NN) was proposed to improve the dynamic behavior of structures for GNSS-PPP method. A complicated numerical simulation involving highly noisy data and 13 experimental cases with different loads were utilized to confirm the efficiency of the proposed model design and the monitoring technique in detecting the dynamic behavior of structures. The results revealed that, when combined with the proposed model, GNSS-PPP method can be used to accurately detect the dynamic behavior of engineering structures as an alternative to relative GNSS method.     

Past,Present and Future of Active Radio Frequency Experiments in Space

Active ionospheric experiments using high-power, high-frequency transmitters, “heaters”, to study plasma processes in the ionosphere and magnetosphere continue to provide new insights into understanding plasma and geophysical proceses. This review describes the heating facilities, past and present, and discusses scientific results from these facilities and associated space missions. Phenomena that have been observed with these facilities are reviewed along with theoretical explanations that have been proposed or are commonly accepted. Gaps or uncertainties in understanding of heating-initiated phenomena are discussed together with proposed science questions to be addressed in the future. Suggestions for improvements and additions to existing facilities are presented including important satellite missions which are necessary to answer the outstanding questions in this field.