Biodosimetry of heavyions by interphase chromosome painting

We report measurements of chromosomal aberrations in peripheral blood lymphocytes from cancer patients undergoingradiotherapy treatment. Patients with cervix or esophageal cancer were treated with 10 MV X-rays produced at a LINAC accelerator, or high-energy carbon ions produced at the HIMAC accelerator at the National Institute for Radiological Sciences (NIRS) in Chiba. Blood samples were obtained before, during, and after the radiation treatment. Chromosomes were prematurely condensed by incubation in calyculin A. Aberrations in chromosomes 2 and 4 were scored after fluorescence in situ hybridization with whole-chromosome probes. Pre-treatment samples were exposed in vitro to X-rays, individual dose-response curves for the induction of chromosomal aberrations were determined, and used as calibration curves to calculate the effective whole-body dose absorbed during the treatment. This calculated dose, based on the calibration curve relative to the induction of reciprocal exchanges, has a sharp increase after the first few fractions of the treatment, then saturates at high doses. Although carbon ions are 2–3 times more effective than X-rays in tumor sterilization, the effective dose was similar to that of X-ray treatment. However, the frequency of complex-type chromosomal exchanges was much higher for patients treated with carbon ions than X-ray.     

Evolution of galaxy cluster scaling from XMM-Newton observation of the galaxy clusters at z ⩾ 0.4

We present here new XMM-Newton observations of 3 relatively cool clusters at z ≈ 0.4, complemented by archival observations of 3 other clusters at similar redshift. We derived the M–T and R–T relations from the hydrostatic equation using an isothermal temperature distribution.     

Solar flare forecasting model supported with artificial neural network techniques

Nowadays operational models for solar activity forecasting are still based on the statistical relationship between solar activity and solar magnetic field evolution. In order to set up this relationship, many parameters have been proposed to be the measures. Conventional measures are based on the sunspot group classification which provides limited information from sunspots. For this reason, new measures based on solar magnetic field observations are proposed and a solar flare forecasting model supported with an artificial neural network is introduced. This model is equivalent to a person with a long period of solar flare forecasting experience.     

The APT on the Polar Orbiting Weather Satellites

The receipt of the Pioneer Award has given me a chance to look back over my professional life and the opportunity to take stock of how I helped shape a small part of the world. While I hope this process entertains my contemporaries, more importantly, I hope it stimulates those that are engaged in actively shaping the present. To describe the need for automatic picture transmission (APT), I must retrace the historical development of meteorological satellites. The idea for weather observations from a satellite originated with a small group of meteorologists at the U.S. Army Signal Corps Research and Development Lab. at Ft. Monmouth, N.J., and resulted in the design of Vanguard II. The Tiros and TOS series of satellites, and the design of Nimbus, followed soon thereafter. However, a faster picture dissemination than was available at that time was needed, and it was this necessity that sparked the development of APT. Nimbus was originally intended to be an operational system, but the advent of simpler, less costly stabilization systems made the Tiros evolution the clear winner. The geosynchronous weather satellites started nearly a decade later and evolved from the NASA Application Technology Satellite (ATS) series. All three systems, existing polar orbiting weather satellites, APT, and geosynchronous weather satellites, have changed meteorology and the reliability of weather forecasting profoundly.     

Simultaneous measurement of multiple radiation-induced protein expression profiles using the Luminex(TM) system.

Space flight results in the exposure of astronauts to a mixed field of radiation composed of energetic particles of varying energies, and biological indicators of space radiation exposure provides a better understanding of the associated long-term health risks. Current methods of biodosimetry have employed the use of cytogenetic analysis for biodosimetry, and more recently the advent of technological progression has led to advanced research in the use of genomic and proteomic expression profiling to simultaneously assess biomarkers of radiation exposure. We describe here the technical advantages of the Luminex(TM) 100 system relative to traditional methods and its potential as a tool to simultaneously profile multiple proteins induced by ionizing radiation. The development of such a bioassay would provide more relevant post-translational dynamics of stress response and will impart important implications in the advancement of space and other radiation contact monitoring.     

Track segment association, fine-step IMM and initialization with Doppler for improved track performance

In this work we present a new track segment association technique to improve track continuity in large-scale target tracking problems where track breakages are common. A representative airborne early warning (AEW) system scenario, which is a challenging environment due to highly maneuvering targets, close target formations, large measurement errors, long sampling intervals, and low detection probabilities, provides the motivation for the new technique. Previously, a tracker using the interacting multiple model (IMM) estimator combined with an assignment algorithm was shown to be more reliable than a conventional Kalman filter based approach in tracking similar targets but it still yielded track breakages due to the difficult environment. In order to combine the broken track segments and improve track continuity, a new track segment association algorithm using a discrete optimization approach is presented. Simulation results show that track segment association yields significant improvements in mean track life as well as in position, speed, and course rms errors. Also presented is a modified one-point initialization technique with range rate measurements, which are typically ignored by other initialization techniques, and a fine-step IMM estimator, which improves performance in the presence of long revisit intervals. Another aspect that is investigated is the benefit of "deep" (multiframe or N-dimensional, with N > 2) association, which is shown to yield significant benefit in reducing the number of false tracks.     

On the structure and dynamics of the thermosphere

Thermospheric temperature, composition and wind measurements from the Dynamics Explorer satellite (DE-2) are interpreted using a three dimensional, multiconstituent spectral model. The analysis accounts for tides driven by the absorbed solar radiation as well as energy and momentum coupling involving the magnetosphere and lower atmosphere. We discuss phenomena associated with the annual tide, polar circulation, magnetic storms and substorms.     

The biological clock of Neurospora in a microgravity environment.

The circadian rhythm of conidiation in Neurospora crassa is thought to be an endogenously derived circadian oscillation; however, several investigators have suggested that circadian rhythms may, instead, be driven by some geophysical time cue(s). An experiment was conducted on space shuttle flight STS-9 in order to test this hypothesis; during the first 7-8 cycles in space, there were several minor alterations observed in the conidiation rhythm, including an increase in the period of the oscillation, an increase in the variability of the growth rate and a diminished rhythm amplitude, which eventually damped out in 25% of the flight tubes. On day seven of flight, the tubes were exposed to light while their growth fronts were marked. Some aspect of the marking process reinstated a robust rhythm in all the tubes which continued throughout the remainder of the flight. These results from the last 86 hours of flight demonstrated that the rhythm can persist in space. Since the aberrant rhythmicity occurred prior to the marking procedure, but not after, it was hypothesized that the damping on STS-9 may have resulted from the hypergravity pulse of launch. To test this hypothesis, we conducted investigations into the effects of altered gravitational forces on conidiation. Exposure to hypergravity (via centrifugation), simulated microgravity (via the use of a clinostat) and altered orientations (via alterations in the vector of a 1 g force) were used to examine the effects of gravity upon the circadian rhythm of conidiation.     

Biological nitrogen fixation under primordial Martian partial pressures of dinitrogen.

Early Earth and early Mars were similar enough such that past geochemical and climatic conditions on Mars may have also been favorable for the origin of life. However, one of the most striking differences between the two planets was the low partial pressure of dinitrogen (pN2) on early Mars (18 mb). On Earth, nitrogen is a key biological element and in many ecosystems the low availability of fixed nitrogen compounds is the main factor limiting growth. Biological fixation of dinitrogen on Earth is a crucial source of fixed nitrogen. Could the low availability of dinitrogen in the primordial Martian atmosphere have prevented the existence, or evolution of Martian microbiota? Azotobacter vinelandii and Azomonas agilis were grown in nitrogen free synthetic medium under various partial pressures of dinitrogen ranging from 780-0 mb (total atmosphere=1 bar). Below 400 mb the biomass, cell number, and growth rate decreased with decreasing pN2. Both microorganisms were capable of growth at a pN2 as low as 5 mb, but no growth was observed at a pN2 < or = 1 mb. The data appear to indicate that biological nitrogen fixation could have occurred on primordial Mars (pN2=18 mb) making it possible for a biotic system to have played a role in the Martian nitrogen cycle. It is possible that nitrogen may have played a key role in the early evolution of life on Mars, and that later a lack of available nitrogen on that planet (currently, pN2=0.2 mb) may have been involved in its subsequent extinction.     

Methods for rotor aerodynamic and dynamic analysis

The complex problems associated with the aerodynamics of blade/vortex interactions, the dynamics of a blade displaced out of its plane of rotation, and the position control of a hovering helicopter are discussed. Simplified models help to describe, and appear to be adequate for analysing, many aspects of these problems. Free wake analyses are shown to be of importance in determining blade loads and performance for hovering flight or for wind turbines. Unsteady aerodynamics and careful definition of the wake formation are required when computing vibratory airloads in forward flight.