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881.
882.
K Dose A Bieger-Dose R Dillmann M Gill O Kerz A Klein H Meinert T Nawroth S Risi C Stridde 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1995,16(8):119-129
The general goal of the experiment was to study the response of anhydrobiotic (metabolically dormant) microorganisms (spores of Bacillus subtilis, cells of Deinococcus radiodurans, conidia of Aspergillus species) and cellular constituents (plasmid DNA, proteins, purple membranes, amino acids, urea) to the extremely dehydrating conditions of open space, in some cases in combination with irradiation by solar UV-light. Methods of investigation included viability tests, analysis of DNA damages (strand breaks, DNA-protein cross-links) and analysis of chemical effects by spectroscopic, electrophoretic and chromatographic methods. The decrease in viability of the microorganisms was as expected from simulation experiments in the laboratory. Accordingly, it could be correlated with the increase in DNA damages. The purple membranes, amino acids and urea were not measurably effected by the dehydrating condition of open space (in the dark). Plasmid DNA, however, suffered a significant amount of strand breaks under these conditions. The response of these biomolecules to high fluences of short wavelength solar UV-light is very complex. Only a brief survey can be given in this paper. The data on the relatively good survival of some of the microorganisms call for strict observance of COSPAR Planetary Protection Regulations during interplanetary space missions. 相似文献
883.
A A Gonzales A C Schuerger C Barford R Mitchell 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1996,18(4-5):5-20
Microbiological contamination of crops within space-based plant growth research chambers has been postulated as a potentially significant problem. Microbial infestations; fouling of Nutrient Delivery System (NDS) fluid loops; and the formation of biofilms have been suggested as the most obvious and important manifestations of the problem. Strict sanitation and quarantine procedures will reduce, but not eliminate, microbial species introduced into plant growth systems in space habitats. Microorganisms transported into space most likely will occur as surface contaminants on spacecraft components, equipment, the crew, and plant-propagative materials. Illustrations of the potential magnitude of the microbiological contamination issue will be drawn from the literature and from documentation of laboratory and commercial field experience. Engineering strategies for limiting contamination and for the development of countermeasures will be described. Microbiological control technologies and NDS hardware will be discussed. Configurations appropriate for microgravity research facilities, as well as anticipated bio-regenerative life support system implementations, will be explored. An efficiently designed NDS, capable of adequately meeting the environmental needs of crop plants in space, is considered to be critical in both the research and operational domains. Recommended experiments, tests, and technology developments, structured to allow the development of prudent engineering solutions also will be presented. 相似文献
884.
One possible method is considered of deriving the well-posed (in the sense of A.N. Tikhonov) solutions to the problems of estimating the aircraft performance characteristics. The method is based on the results of measurements of the state parameters and geophysical conditions of the employment of the rocket-and-space facilities under conditions of uncertainty of the a priori data on measurement errors. 相似文献
885.
Y Kitaya A Tani M Kiyota I Aiga 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1994,14(11):281-284
In order to obtain basic data for construction of a plant cultivation system incorporating a mushroom cultivation subsystem in the CELSS, plant growth and atmospheric CO2 balance in the system were investigated. The plant growth was promoted by a high level of CO2 which resulted from the respiration of the mushroom mycelium in the system. The atmospheric CO2 concentration inside the system changed significantly due to the slight change in the net photosynthetic rate of plants and/or the respiration rate of the mushroom when the plant cultivation system combined directly with the mushroom cultivation subsystem. 相似文献
886.
I Block A Wolke W Briegleb 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1994,14(8):21-34
The acellular slime mold Physarum polycephalum is used as a model system to investigate the graviresponse of single cells which possess no receptors specialized for the perception of gravity. To obtain insights into the gravity-signal transduction mechanism the light response of the cell is used: Macroplasmodia of the slime mold show clear geo- and phototaxes. Gravity increases and white light decreases transiently the contraction frequency of plasmodial strands whereby both responses follow the same time pattern. Since mitochondria play a major role in changing the contraction rhythm in response to light and gravity stimuli, the simultaneous and subsequent inductions of the opposing light and gravity responses and their mutual influences on one another were investigated. The experiments were performed in weightlessness (0 g)--simulated on the fast-rotating clinostat as well as in actual weightlessness during the IML-1 Space Shuttle mission. The results indicate that mitochondria (chondriome) are part of the acceleration-stimulus reaction chain in Physarum. Two models for a direct gravireceptor mechanism are discussed. 相似文献
887.
R. C. Reynolds P. D. Anz-Meador G. W. Ojakangas 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1991,11(12):29-32
In reviewing discussions of future directions for space activity, it becomes obvious that there are a large number of groups formulating a wide diversity of plans for the future use of space. These plan alternatives are being made to account for user needs, technology development constraints, economic constraints, and launch support, and each of the plans will have direct or indirect effects on the orbital debris environment in terms of mass to orbit, deposition of operational debris, and control of accidental breakups. Thus it is important to develop the ability to project future debris states for a range of possible space traffic scenarios. The impact that these possible traffic environments would have on space operations forms the basis for studies of alternative options for the usage of space. In this paper, the effects on the orbital debris environment of a base-line mission model and two alternatives are investigated, using a numerical debris environment simulation code under development at JSC. 相似文献
888.
A N Boyandin T I Lobova L Yu Popova N S Pechurkin 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,31(7):1763-1768
It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (AprLux+) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g l-1) taken from Lake Shira (Khakasia, Russia). The survival of the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor-ampicillin (50 micrograms/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The populations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 micrograms/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 micrograms/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retails the recombinant plasmid. 相似文献
889.
I Gillette-Ferguson D G Ferguson K D Poss S J Moorman 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,32(8):1641-1646
Little is known about the effect of microgravity on gene expression, particularly in vivo during embryonic development. Using transgenic zebrafish that express the gfp gene under the influence of a beta-actin promoter, we examined the affect of simulated-microgravity on GFP expression in the heart. Zebrafish embryos, at the 18-20 somite-stage, were exposed to simulated-microgravity for 24 hours. The intensity of GFP fluorescence associated with the heart was then determined using fluorescence microscopy. Our measurements indicated that simulated-microgravity induced a 23.9% increase in GFP-associated fluorescence in the heart. In contrast, the caudal notochord showed a 17.5% increase and the embryo as a whole showed only an 8.5% increase in GFP-associated fluorescence. This suggests that there are specific effects on the heart causing the more dramatic increase. These studies indicate that microgravity can influence gene expression and demonstrate the usefulness of this in vivo model of 'reporter-gene' expression for studying the effects of microgravity. 相似文献
890.
As the USA, Europe and other nations embark on a new voyage of exploration to the Moon, Mars and beyond, they should lay the foundations and establish precedents that invite a host of participants and followers. We argue that international cooperation, driven by foreign-policy and cost-sharing considerations, has taken a prominent role but must be pragmatically and flexibly balanced with economic and strategic self-interest. Since exploration visions are likely to differ, the steps each country will pursue, the funding provided, and schedules followed will also differ. To support an enduring exploration vision, it will be important to remain flexible to changing priorities and amenable to the inclusion of new, non-traditional participants. Open-systems principles and metaprinciples should be employed at all levels—hardware, software, programmatic, political and cultural. Equally important, national leadership and decision makers should be mindful of the potential pitfalls that might undermine the venture. While the new vision inspires us all, it will take creativity, resourcefulness, hard work and cooperation to succeed. 相似文献