Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

基于磨粒分析方法的发动机磨损故障智能诊断技术

磨粒是研究磨损状态时最直接、最重要的信息元，通过对滑油中的磨粒进行监测与分析来判断机械设备的磨损情况，可以预防并监测机械设备的磨损故障。本文运用显微形态学方法建立了一套磨粒显微特征描述体系，以提取磨粒信息并进行磨损故障的模式识别；并结合摩擦学理论和人工智能方法，实现对发动机磨损故障的智能诊断和预测。     

The Radarsat Antarctic Mapping Mission

The Radarsat Earth-observation satellite was launched on November 4, 1995 aboard a Delta 2 rocket. Equipped with a sophisticated synthetic aperture radar (SAR), Radarsat can produce images of extraordinary clarity even through clouds, smog, haze, smoke, and darkness. The SAR has a variety of operating modes. It can be adjusted to produce swathes between 35 and 500 km in width, with ground resolutions from 100 m to as low as eight. In addition, the beam can be steered at angles up to 49° from the satellite's nadir vector, giving it the unique ability to image areas it is not directly overflying. In exchange for the launch, CSA agreed to provide NASA with access to the SAR data, and to execute a 180° yaw-around of Radarsat twice during its lifetime to map the Antarctic continent. Preliminary results from the first of these Antarctic Mapping Missions, dubbed AMM-1, are presented     

Design of the TRIO system-on-chip for aerospace

Several design and testing aspects of the TRIO smart sensor data acquisition chip, developed by JHU/APL for NASA spacecraft applications are presented. TRIO includes a 10 bit self-corrected analog-to-digital converter (ADC), 16/32 analog inputs, a front end multiplexer with selectable aquisition time, a current source, memory, serial and parallel bus, and control logic. So far TRIO is used in many missions including Contour, Messenger, Stereo, Pluto, and the generic JPL X2000 spacecraft bus.     

Determination of aerodynamic forces on satellites by theory and wind tunnel experiments

Theoretical and experimental aspects of satellite aerodynamics in free molecular flow have been studied. A FORTRAN-program package has been developed to calculate free molecular aerodynamic force and moment coefficients of general and special convex shapes, using an approximation of the surfaces by plane triangular elements.As a special case for concave bodies, the drag of a satellite of TD1A-similar shape has been investigated by an approximate theory and by wind tunnel experiments. Comparisons are made between experimental results, the approximate theory and exact theory.     

The Extreme Ultraviolet Imager Investigation for the IMAGE Mission

The Extreme Ultraviolet Imager (EUV) of the IMAGE Mission will study the distribution of He⁺ in Earth's plasmasphere by detecting its resonantly-scattered emission at 30.4 nm. It will record the structure and dynamics of the cold plasma in Earth's plasmasphere on a global scale. The 30.4-nm feature is relatively easy to measure because it is the brightest ion emission from the plasmasphere, it is spectrally isolated, and the background at that wavelength is negligible. Measurements are easy to interpret because the plasmaspheric He⁺ emission is optically thin, so its brightness is directly proportional to the He⁺ column abundance. Effective imaging of the plasmaspheric He⁺ requires global `snapshots in which the high apogee and the wide field of view of EUV provide in a single exposure a map of the entire plasmasphere. EUV consists of three identical sensor heads, each having a field of view 30° in diameter. These sensors are tilted relative to one another to cover a fan-shaped field of 84°×30°, which is swept across the plasmasphere by the spin of the satellite. EUVs spatial resolution is 0.6° or 0.1 R _E in the equatorial plane seen from apogee. The sensitivity is 1.9 count s^–1 Rayleigh^–1, sufficient to map the position of the plasmapause with a time resolution of 10 min.     

Filtering of discontinuous processes arising in marine integratednavigation systems

A refined stochastic model for the errors of the Loran-C radio navigation aid is described, and it is shown how this model can be used to improve the performance of integrated navigation systems. In addition to the usual propagation errors, Loran-C time of arrival measurements are occasionally plagued with sudden intermittent errors of a particular magnitude and caused by receiver cycle selection errors. These result in sudden large jumps in the calculated position solution. The Loran-C error has been modeled as the sum of a diffusion process, representing the normal propagating errors, and a pure jump process of Poisson type, representing the cycle selection errors. A simple integrated navigation system is then described, based on the Loran-C model and the standard dead reckoning (heading and speed) system model. Assuming that the observed process is governed by a linear stochastic difference equation, a recursive linear unbiased minimum variance filter is developed, from which the Loran-C and dead reckoning errors, and hence position and velocity, can be estimated     

The effect of small vibrations on Marangoni convection and the free surface of a liquid bridge

The effects of small vibrations on Marangoni convection were investigated experimentally using a liquid bridge of 5 cSt silicone oil with a disk diameter of 7.0 mm, and an aspect ratio close to 0.5. Experiments were performed to determine the critical temperature difference data for no vibration case and with small vibrations applied. The experimental results have shown that the effect of small vibrations on the onset of oscillatory flow is small since the critical temperature difference data for different aspect ratios were not affected by the vibrations. To clarify the surface oscillation phenomena induced by external vibrations, a 3-D numerical simulation model was also developed using a level set algorithm to predict the surface oscillations of isothermal silicone oil bridges. By subjecting the liquid bridge to small vibrations, the surface oscillation characteristics were predicted numerically, and the numerical results compared well with the predictions of an analytical model proposed previously. Furthermore, the effect of small vibrations on the surface vibration amplitude of the liquid bridge is also discussed.     

Surface characteristics of spacecraft components affect the aggregation of microorganisms and may lead to different survival rates of bacteria on Mars landers

Layers of dormant endospores of Bacillus subtilis HA101 were applied to eight different spacecraft materials and exposed to martian conditions of low pressure (8.5 mbar), low temperature (-10 degrees C), and high CO(2) gas composition and irradiated with a Mars-normal ultraviolet (UV-visible- near-infrared spectrum. Bacterial layers were exposed to either 1 min or 1 h of Mars-normal UV irradiation, which simulated clear-sky conditions on equatorial Mars (0.1 tau). When exposed to 1 min of Mars UV irradiation, the numbers of viable endospores of B. subtilis were reduced three to four orders of magnitude for two brands of aluminum (Al), stainless steel, chemfilm-treated Al, clear-anodized Al, and black-anodized Al coupons. In contrast, bacterial survival was reduced only one to two orders of magnitude for endospores on the non-metal materials astroquartz and graphite composite when bacterial endospores were exposed to 1 min of Mars UV irradiation. When bacterial monolayers were exposed to 1 h of Mars UV irradiation, no viable bacteria were recovered from the six metal coupons listed above. In contrast, bacterial survival was reduced only two to three orders of magnitude for spore layers on astroquartz and graphite composite exposed to 1 h of Mars UV irradiation. Scanning electron microscopy images of the bacterial monolayers on all eight spacecraft materials revealed that endospores of B. subtilis formed large aggregates of multilayered spores on astroquartz and graphite composite, but not on the other six spacecraft materials. It is likely that the formation of multilayered aggregates of endospores on astroquartz and graphite composite is responsible for the enhanced survival of bacterial cells on these materials.     

The Rosat mission

The primary scientific objective of the ROSAT mission is to perform the first all sky survey with an imaging X-ray telescope leading to an improvement in sensitivity by several orders of magnitude compared with previous surveys. Consequently a large number of new sources (> 10⁵) will be discovered and located with an accuracy of 1 arcmin. After completion of the survey which will take about half a year the instrument will be used for detailed observations of selected targets.The X-ray telescope consists of a fourfold nested Wolter type I mirror system with 80 cm aperture and 240 cm focal length, and three focal plane detectors. In the baseline version these will be imaging proportional counters (0.1 – 2 keV) providing a field of view of 2⁰ × 2⁰.