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981.
The identification of extant and, in some cases, extinct bacterial life is most convincingly and efficiently performed with modern high-resolution microscopy. Epifluorescence microscopy of microbial autofluorescence or in conjunction with fluorescent dyes is among the most useful of these techniques. We explored fluorescent labeling and imaging of bacteria in rock and soil in the context of in situ life detection for planetary exploration. The goals were two-fold: to target non-Earth-centric biosignatures with the greatest possible sensitivity and to develop labeling procedures amenable to robotic implementation with technologies that are currently space qualified. A wide panel of commercially available dyes that target specific biosignature molecules was screened, and those with desirable properties (i.e., minimal binding to minerals, strong autofluorescence contrast, no need for wash steps) were identified. We also explored the potential of semiconductor quantum dots (QDs) as bacterial and space probes. A specific instrument for space implementation is suggested and discussed. 相似文献
982.
Searching for life on Mars: selection of molecular targets for ESA's aurora ExoMars mission 总被引:1,自引:0,他引:1
Parnell J Cullen D Sims MR Bowden S Cockell CS Court R Ehrenfreund P Gaubert F Grant W Parro V Rohmer M Sephton M Stan-Lotter H Steele A Toporski J Vago J 《Astrobiology》2007,7(4):578-604
The European Space Agency's ExoMars mission will seek evidence of organic compounds of biological and non-biological origin at the martian surface. One of the instruments in the Pasteur payload may be a Life Marker Chip that utilizes an immunoassay approach to detect specific organic molecules or classes of molecules. Therefore, it is necessary to define and prioritize specific molecular targets for antibody development. Target compounds have been selected to represent meteoritic input, fossil organic matter, extant (living, recently dead) organic matter, and contamination. Once organic molecules are detected on Mars, further information is likely to derive from the detailed distribution of compounds rather than from single molecular identification. This will include concentration gradients beneath the surface and gradients from generic to specific compounds. The choice of biomarkers is informed by terrestrial biology but is wide ranging, and nonterrestrial biology may be evident from unexpected molecular distributions. One of the most important requirements is to sample where irradiation and oxidation are minimized, either by drilling or by using naturally excavated exposures. Analyzing regolith samples will allow for the search of both extant and fossil biomarkers, but sequential extraction would be required to optimize the analysis of each of these in turn. 相似文献
983.
Two terrestrial environments that have been proposed as analogs for the iron oxide precipitation in the Meridiani Planum region of Mars include the Rio Tinto precipitates and southern Utah marble concretions. Samples of two typical Utah iron oxide concretions and iron oxide precipitates in contact with biofilms from Rio Tinto have been studied to determine whether evidence could be found for biomediation in the precipitation process and to identify likely locations for fossil microorganisms. Scanning electron microscopy, energy dispersive X-ray, and gas chromatography-mass spectrometry (GC-MS) were used to search for biosignatures in the Utah marbles. The precipitation of iron oxides resembles known biosignatures, though organic compounds could not be confirmed with GC-MS analysis. In contrast, textural variations induced by biological activity are abundant in the modern Rio Tinto samples. Although no compelling evidence of direct or indirect biomediation was found in the Utah marbles, the ultrastructure of the iron oxide cement in the concretion suggests an inward growth during concretion precipitation from an initially spherical redox front. No indication for growth from a physical nucleus was found. 相似文献
984.
O. Le Contel S. Perraut A. Roux R. Pellat A. Korth 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2000,25(12):2395-2406
Thin Current Sheets (TCS) are regularly formed prior to substorm breakup, even in the near-Earth plasma sheet, as close as the geostationary orbit. A self-consistent kinetic theory describing the response of the plasma sheet to an electromagnetic perturbation is given. This perturbation corresponds to an external forcing, for instance caused by the solar wind (not an internal instability). The equilibrium of the configuration of this TCS in the presence of a time varying perturbation is shown to produce a strong parallel thermal anisotropy (T T) of energetic electrons and ions (E>50keV) as well as an enhanced diamagnetic current carried by low energy ions (E<50keV). Both currents tend to enhance the confinement of this current sheet near the magnetic equator. These results are compared with data gathered by GEOS-2 at the geostationary orbit, where the magnetic signatures of TCS, and parallel anisotropics are regularly observed prior to breakup. By ensuring quasi-neutrality everywhere we find, when low frequency electromagnetic perturbations are applied, that although the magnetic field line remains an equipotential to the lowest order in Te/Ti, a field-aligned potential drop exists to the next order in (Te/Ti). Thus the development of a TCS implies the formation of a field-aligned potential drop ( few hundred volts) to ensure the quasi-neutrality everywhere. For an earthward directed pressure gradient, a field-aligned electric field, directed towards the ionosphere, is obtained, on the western edge of the perturbation (i.e. western edge of the current sheet). Thus field aligned beams of electrons are expected to flow towards the equatorial region on the western edge of the current sheet. We study the stability of these electron beams and show that they are unstable to “High Frequency” (HF) waves. These “HF” waves are regularly observed at frequencies of the order of the proton gyrofrequency (fH+) just before, or at breakup. The amplitude of these HF waves is so large that they can produce a strong pitch-angle diffusion of energetic ions and a spatial diffusion that leads to a reduction of the diamagnetic current. The signature of a fast ion diffusion is indeed regularly observed during the early breakup; it coincides with the sudden development of large amplitude transient fluctuations, ballooning modes, observed at much lower frequencies (fH+). These results suggest that the HF waves, generated by field-aligned electron beams, provide the dissipation which is necessary to destabilize low frequency (ballooning) modes. 相似文献
985.
Green J.L. Benson R.F. Fung S.F. Taylor W.W.L. Boardsen S.A. Reinisch B.W. Haines D.M. Bibl K. Cheney G. Galkin I.A. Huang X. Myers S.H. Sales G.S. Bougeret J.-L. Manning R. Meyer-Vernet N. Moncuquet M. Carpenter D.L. Gallagher D.L. Reiff P.H. 《Space Science Reviews》2000,91(1-2):361-389
The Radio Plasma Imager (RPI) will be the first-of-its kind instrument designed to use radio wave sounding techniques to perform repetitive remote sensing measurements of electron number density (N
e) structures and the dynamics of the magnetosphere and plasmasphere. RPI will fly on the Imager for Magnetopause-to-Aurora Global Exploration (IMAGE) mission to be launched early in the year 2000. The design of the RPI is based on recent advances in radio transmitter and receiver design and modern digital processing techniques perfected for ground-based ionospheric sounding over the last two decades. Free-space electromagnetic waves transmitted by the RPI located in the low-density magnetospheric cavity will be reflected at distant plasma cutoffs. The location and characteristics of the plasma at those remote reflection points can then be derived from measurements of the echo amplitude, phase, delay time, frequency, polarization, Doppler shift, and echo direction. The 500 m tip-to-tip X and Y (spin plane) antennas and 20 m Z axis antenna on RPI will be used to measures echoes coming from distances of several R
E. RPI will operate at frequencies between 3 kHz to 3 MHz and will provide quantitative N
e values from 10–1 to 105 cm–3. Ray tracing calculations, combined with specific radio imager instrument characteristics, enables simulations of RPI measurements. These simulations have been performed throughout an IMAGE orbit and under different model magnetospheric conditions. They dramatically show that radio sounding can be used quite successfully to measure a wealth of magnetospheric phenomena such as magnetopause boundary motions and plasmapause dynamics. The radio imaging technique will provide a truly exciting opportunity to study global magnetospheric dynamics in a way that was never before possible. 相似文献
986.
The Radio Plasma Imager investigation on the IMAGE spacecraft 总被引:1,自引:0,他引:1
Reinisch B.W. Haines D.M. Bibl K. Cheney G. Galkin I.A. Huang X. Myers S.H. Sales G.S. Benson R.F. Fung S.F. Green J.L. Boardsen S. Taylor W.W.L. Bougeret J.-L. Manning R. Meyer-Vernet N. Moncuquet M. Carpenter D.L. Gallagher D.L. Reiff P. 《Space Science Reviews》2000,91(1-2):319-359
Radio plasma imaging uses total reflection of electromagnetic waves from plasmas whose plasma frequencies equal the radio sounding frequency and whose electron density gradients are parallel to the wave normals. The Radio Plasma Imager (RPI) has two orthogonal 500-m long dipole antennas in the spin plane for near omni-directional transmission. The third antenna is a 20-m dipole along the spin axis. Echoes from the magnetopause, plasmasphere and cusp will be received with the three orthogonal antennas, allowing the determination of their angle-of-arrival. Thus it will be possible to create image fragments of the reflecting density structures. The instrument can execute a large variety of programmable measuring options at frequencies between 3 kHz and 3 MHz. Tuning of the transmit antennas provides optimum power transfer from the 10 W transmitter to the antennas. The instrument can operate in three active sounding modes: (1) remote sounding to probe magnetospheric boundaries, (2) local (relaxation) sounding to probe the local plasma frequency and scalar magnetic field, and (3) whistler stimulation sounding. In addition, there is a passive mode to record natural emissions, and to determine the local electron density, the scalar magnetic field, and temperature by using a thermal noise spectroscopy technique. 相似文献
987.
Beasley Eric W. Ward Harold R. 《IEEE transactions on aerospace and electronic systems》1968,(3):468-473
A brief statement of the sea clutter problem in surface-search radar operation illustrates the need for some form of signal-to-clutter enhancement. Post-detection integration used in the simpler radars is limited by the pulse-to-pulse correlation of the clutter. Analysis of the effect of changing frequency from pulse to pulse leads to an expression for the correlation between pulses in the sequence. Knowing this correlation, the reduction in the fluctuating clutter component produced by integration can be determined. This is described by an equivalent number of independent pulses, Nc. For the particular case of sinusoidal modulation of the transmitted frequency, N6 is computed. The critical dependecne of Nc upon the modulating frequency fm is illustrated by spectrum photographs. Choice of an optimum fm is discussed. The results of computations of N4 for optimum fm are presented as a family of normalized curves. These data permit the tradeoff of the radar parameters against their quantitative effect on radar performance. 相似文献
988.
M.E. Yahia R.E. Tolba W.M. Moslem 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2021,67(4):1412-1424
Super rogue ion-acoustic waves are proposed as a physical catalyst for the heavy hydrocarbon ions formation in the Titan ionosphere. We justified that analytically and numerically by probing a Titan referenced plasma system, consists of the most abundant positive ions and superthermal electrons. A solution of the nonlinear Schrödinger equation (NLSE) has provided us by the plasma (un) stable regions at altitude 900–1200 km from the Titan surface with superthermal parameter values, relative ion to electron densities, and temperature ratio variations. Our results are not only agreed with the Cassini data but also predict a chemistry independent approach for the heavy hydrocarbons’ formation conditions. 相似文献
989.
Cosmic Research - Using the Meteor M2 low-orbit satellite, the dynamics of the boundaries of the auroral oval on the nightside during the magnetic storm observed from May 27 to 29, 2017, was... 相似文献
990.
Interim results of a study on use of the tethered propellant resupply technique on the space station are summarized. The acceleration produced by a gravity-gradient-stabilized tether can predictably settle propellants and thereby simplify propellant resupply of vehicles when compared to zero-g techniques. Separation of the gas and liquid phases by settling enables performance of liquid acquisition and gas venting without special hardware in the propellant tanks and without special procedures. The primary requirement for propellant transfer is control of liquid sloshing to maintain liquid over the supply tank outlet and gas over the receiver tank vent. Ultimately, the decision to use this technique on the space station may depend upon the capability to adjust depot logistic operations to a tether. 相似文献