Immobilization induces a very rapid increase in osteoclast activity

We studied in a randomized, strictly controlled cross-over design, the effects of 6 days 6 degrees head-down tilt bed rest (HDT) in eight male healthy subjects in our metabolic ward. The study consisted of two periods (phases) of 11 days each in order to allow for the test subjects being their own controls. Both study phases were identical with respect to environmental conditions, study protocol and diet. Two days before arriving in the metabolic ward the subjects started with a diet. The diet was continued in the metabolic ward. The metabolic ward period (1l days) was divided into three parts: 4 ambulatory days, 6 days either HDT or control and 1 recovery day. Continuous urine collection started on the first day in the metabolic ward to analyze calcium excretion and bone resorption markers. On the 2nd ambulatory day in the metabolic ward and on the 5th day in HDT or control blood was drawn to analyze serum calcium, parathyroid hormone, and bone formation markers. Urinary calcium excretion was, as early as the first day in immobilization, increased (p<0.01). CTX- and NTX-excretion stayed unchanged in the first 24 h in HDT compared to the control. But already on the 2nd day of immobilization, both bone resorption markers significantly increased. We conclude from these results--pronounced rise of bone resorption markers--that already 24 h of immobilization induce a significant rise in osteoclast activity in healthy subjects. Thus, it appears possible to use short-term bed rest studies as a first step for the development of countermeasures to immobilization.     

Experimental support for an immunological approach to the search for life on other planets

We propose a three-phase approach to test for evidence of life in extraterrestrial samples. The approach capitalizes on the flexibility, sensitivity, and specificity of antibody-antigen interactions. Data are presented to support the first phase, in which various extraction protocols are compared for efficiency, and in which a preliminary suite of antibodies are tested against various antigens. The antigens and antibodies were chosen on the basis of criteria designed to optimize the detection of extraterrestrial biomarkers unique to living or once-living organisms.     

Recurrent isolation of hydrogen peroxide-resistant spores of Bacillus pumilus from a spacecraft assembly facility

While the microbial diversity of a spacecraft assembly facility at the Jet Propulsion Laboratory (Pasadena, CA) was being monitored, H2O2-resistant bacterial strains were repeatedly isolated from various surface locations. H2O2 is a possible sterilant for spacecraft hardware because it is a low-temperature process and compatible with various modern-day spacecraft materials, electronics, and components. Both conventional biochemical testing and molecular analyses identified these strains as Bacillus pumilus. This Bacillus species was found in both unclassified (entrance floors, anteroom, and air-lock) and classified (floors, cabinet tops, and air) locations. Both vegetative cells and spores of several B. pumilus isolates were exposed to 5% liquid H2O2 for 60 min. Spores of each strain exhibited higher resistance than their respective vegetative cells to liquid H2O2. Results indicate that the H2O2 resistance observed in both vegetative cells and spores is strain-specific, as certain B. pumilus strains were two to three times more resistant than a standard Bacillus subtilis dosimetry strain. An example of this trend was observed when the type strain of B. pumilus, ATCC 7061, proved sensitive, whereas several environmental strains exhibited varying degrees of resistance, to H2O2. Repeated isolation of H2O2-resistant strains of B. pumilus in a clean-room is a concern because their persistence might potentially compromise life-detection missions, which have very strict cleanliness and sterility requirements for spacecraft hardware.     

COSAC onboard Rosetta: a bioastronomy experiment for the short-period comet 67P/Churyumov-Gerasimenko

The COSAC (Cometary Sampling and Composition Experiment) onboard the Rosetta mission is a combined gas chromatograph (GC)-mass spectrometer (MS). It is situated on Philae, the lander of the mission, which is intended to land on the nucleus of comet 67P/Churyumov- Gerasimenko. The purpose of the experiment is to analyze the volatile fraction of soil samples retrieved by a drill. For investigation, the samples will be pyrolysed, and the emanating gases fed into a GC, into an MS, or the combination of both. In the first part of this paper, the bioastronomical relevance of such measurements is outlined. In the second part the details of the hardware and its performance are described.     

考古,寻找进化的雕塑——对话罗伯特·巴克（下）

斯：你不仅是知名的古生物学家，而且是一位有造诣的艺术家。你是怎样学习绘画的？ 巴：任何人都能作画。绘画的能力不在你的指尖，而在你的眼睛里。如果你能看见结构，如果你能看见形式，你就能把它记录下来。我上解剖学课，就把学生带到动物园，对他们说：“画那头美洲豹。”“我不会画。”他们说。“你们能看见那头美洲豹吗？”“能看见。”“肘部是直的还是弯的？“‘噢，弯的”“踝关节是弯的吗？”“是的。”“它比狗的踝关节还要弯吧？”“不错。”“把它画下来。”要是你懂得怎样看结构，能在你的头脑中大概画下一系列东西，你就能把它画到纸上。     

考古，寻找进化的雕塑——对话罗伯特·巴克（上）

达林·R．斯蒂尔（以下简称“斯”）：你刚上四年级的时候就决定研究恐龙。是受到什么影响吗？     

具有无源控制空腔时正激波/湍流附面层干扰的数值模拟

采用雷诺平均N-S方程和B/L代数湍流模型计算了具有无源控制空腔时正激波/湍流附面层干扰流场。计算与实验结果的比较表明,本文方法可较准确地预测激波结构、激波与附面层干扰区流动基本特征及波后流动分离状态、激波位置、波前马赫数等参数。      

辨识传递函数模型的应用程序及技术问题探讨

我们用BASIC语言设计了根据频响数据辨识传递函数模型（以下简称模型）的应用程序。在计算机上对八个模型的频响数据进行了辨识,结果较吻合。 一、原理和误差判定准则 1.原理 应用最小二乘原理,使试验结果C（jω₁）和辨识结果G_a（jω₁）的误差平方和为最小。      

管路中常温推进剂的两相充填特性仿真

常温推进剂在管路中的两相充填特性由于气液相间的相互作用而难以预测.为拓展液体火箭发动机瞬态特性模块化通用仿真模型库对两相充填的仿真能力,基于Modelica模块化建模思想开发了一维有限体积的两相充填管路模型,其中采用等效流容方程计算压力,使用Volume of Fluid(VOF)法捕捉气液界面.对流项离散格式的比较表...     

基于特征优化与改进KNN的航空发动机故障诊断

为有效解决航空发动机气路故障诊断难题,建立了基于特征优化与改进KNN的航空发动机气路故障诊断模型。利用特征优化算法对发动机故障特征进行处理,包括特征增维与近邻成分分析算法;将特征优化后的特征输入改进KNN算法,建立基于特征优化与改进KNN算法的故障诊断模型;为验证所建立故障诊断模型的准确性,在四台CFM56-7FB发动机数据上进行实验验证,结果表明：基于特征优化与改进KNN算法的故障诊断模型的准确率可达98%以上,能够达到智能诊断的目的。