Effect of track structure and radioprotectors on the induction of oncogenic transformation in murine fibroblasts by heavy ions

The oncogenic potential of high-energy ⁵⁶Fe particles (1 GeV/nucleon) accelerated with the Alternating Gradient Synchrotron at the Brookhaven National Laboratory was examined utilizing the mouse C3H 10T1/2 cell model. The dose-averaged LET for high-energy ⁵⁶Fe is estimated to be 143 keV/μm with the exposure conditions used in this study. For ⁵⁶Fe ions, the maximum relative biological effectiveness (RBE_max) values for cell survival and oncogenic transformation were 7.71 and 16.5 respectively. Compared to 150 keV/μm ⁴He nuclei, high-energy ⁵⁶Fe nuclei were significantly less effective in cell killing and oncogenic induction. The prostaglandin E₁ analog misoprostol, an effective oncoprotector of C3H 10T1/2 cells exposed to X rays, was evaluated for its potential as a radioprotector of oncogenic transformation with high-energy ⁵⁶Fe. Exposure of cells to misoprostol did not alter ⁵⁶Fe cytotoxicity or the rate of ⁵⁶Fe-induced oncogenic transformation.     

Tissue responses to low protracted doses of high LET radiations or photons: early and late damage relevant to radio-protective countermeasures.

Early and late murine tissue responses to single or fractionated low doses of heavy charged particles, fission-spectrum neutrons or gamma rays are considered. Damage to the hematopoietic system is emphasized, but results on acute lethality, host response to challenge with transplanted leukemia cells and life-shortening are presented. Low dose rates per fraction were used in some neutron experiments. Split-dose lethality studies (LD 50/30) with fission neutrons indicated greater accumulation of injury during a 9 fraction course (over 17 days) than was the case for gamma-radiation. When total doses of 96 or 247 cGy of neutrons or gamma rays were given as a single dose or in 9 fractions, a significant sparing effect on femur CFU-S depression was observed for both radiation qualities during the first 11 days, but there was not an earlier return to normal with dose fractionation. During the 9 fraction sequence, a significant sparing effect of low dose rate on CFU-S depression was observed in both neutron and gamma-irradiated mice. CFU-S content at the end of the fractionation sequence did not correlate with measured LD 50/30. Sustained depression of femur and spleen CFU-S and a significant thrombocytopenia were observed when a total neutron dose of 240 cGy was given in 72 fractions over 24 weeks at low dose rates. The temporal aspects of CFU-S repopulation were different after a single versus fractionated neutron doses. The sustained reduction in the size of the CFU-S population was accompanied by an increase in the fraction in DNA synthesis. The proliferation characteristics and effects of age were different for radial CFU-S population closely associated with bone, compared with the axial population that can be readily aspirated from the femur. In aged irradiated animals, the CFU-S proliferation/redistribution response to typhoid vaccine showed both an age and radiation effect. After high single doses of neutrons or gamma rays, a significant age- and radiation-related deficiency in host defense mechanisms was detected by a shorter mean survival time following challenge with transplantable leukemia cells. Comparison of dose-response curves for life shortening after irradiation with fission-spectrum neutrons or high energy silicon particles indicated high initial slopes for both radiation qualities at low doses, but for higher doses of silicon, the effect per Gy decreased to a value similar to that for gamma rays. The two component life-shortening curve for silicon particles has implications for the potential efficacy of radioprotectants. Recent studies on protection against early and late effects by aminothiols, prostaglandins, and other compounds are discussed.     

Use of Airborne Monopulse Radar as a Low Approach Aid

The potential of airborne radar to provide pictorial displays as an aid to low approach has stimulated invention of several aircraft approach systems. Early developments are reviewed briefly, and an experiment in producing and flight testing a two-dimensional, range and azimuth, pictorial radar display is described. The monopulse radar equipment and a monopulse display improvement (MDI) technique used in the flight test to enhance the B-scope display are also described in some detail. Representative radar scope photographs are used to illustrate the display available in the aircraft.     

The Segmented Aperture Synthetic Aperture Radar (SASAR)

A new concept in synthetic aperture radar, called SASAR, which uses a segmented aperture, is described. Use of the segmented aperture allows appreciable extra receiving antenna gain to be realized. Each subarray of the receive antenna is equal in length to the transmit antenna; the system performance is increased approximately by a factor equal to the number of subarrays. To allow array combination of the subarray signal outputs requires a phase-shift factor (varying with azimuth) to be applied to each subarray signal. A digital implementation of this preprocessor is sketched out; it uses a push-down storage stack to store the range histories for a synthetic aperture from each subarray. Appropriate phase shifts are added to the stacks and a sum of stack values then provides the combined output range history sequence. Possibilities of using analog delay lines for preprocessing are also discussed. Pattern errors due to subarray size and receive array near field are examined and constraints are given.     

An improved Green's function for ion beam transport.

Ion beam transport theory allows testing of material transmission properties in the laboratory environment generated by particle accelerators. This is a necessary step in materials development and evaluation for space use. The approximations used in solving the Boltzmann transport equation for the space setting are often not sufficient for laboratory work and those issues are the main emphasis of the present work. In consequence, an analytic solution of the linear Boltzmann equation is pursued in the form of a Green's function allowing flexibility in application to a broad range of boundary value problems. It has been established that simple solutions can be found for high charge and energy (HZE) ions by ignoring nuclear energy downshifts and dispersion. Such solutions were found to be supported by experimental evidence with HZE ion beams when multiple scattering was added. Lacking from the prior solutions were range and energy straggling and energy downshift with dispersion associated with nuclear events. Recently, we have found global solutions including these effects providing a broader class of HZE ion solutions.     

Coronal radio-sounding detection of a CME during the 1997 Galileo solar conjunction

Frequency fluctuations of the Galileo S-band radio signal were recorded nearly continuously during the spacecraft’s solar conjunction from December 1996 to February 1997. A strong propagating disturbance, most probably associated with a coronal mass ejection (CME), was detected on 7 February when the radio ray path proximate point was on the west solar limb at about 54 solar radii from the Sun. The CME passage through the line of sight is characterized by a significant increase in the fluctuation intensity of the recorded frequency and by an increase in the plasma speed from about 234 km s⁻¹ up to about 755 km s⁻¹. These velocity estimates are obtained from a correlation analysis of frequency fluctuations recorded simultaneously at two widely-separated ground stations. The density turbulence power spectrum is found to steepen behind the CME front. The Galileo radio-sounding data are compared with SOHO/LASCO observations of the CME in the corona and with WIND spacecraft data near the Earth’s orbit.