Gene Transfer and the Reconstruction of Life’s Early History from Genomic Data

The metaphor of the unique and strictly bifurcating tree of life, suggested by Charles Darwin, needs to be replaced (or at least amended) to reflect and include processes that lead to the merging of and communication between independent lines of descent. Gene histories include and reflect processes such as gene transfer, symbioses and lineage fusion. No single molecule can serve as a proxy for the tree of life. Individual gene histories can be reconstructed from the growing molecular databases containing sequence and structural information. With some simplifications these gene histories can be represented by furcating trees; however, merging these gene histories into web-like organismal histories, including the transfer of metabolic pathways and cell biological innovations from now-extinct lineages, has yet to be accomplished. Because of these difficulties in interpreting the record retained in molecular sequences, correlations with biochemical fossils and with the geological record need to be interpreted with caution. Advances to detect and pinpoint transfer events promise to untangle at least a few of the intertwined histories of individual genes within organisms and trace them to the organismal ancestors. Furthermore, analysis of the shape of molecular phylogenetic trees may point towards organismal radiations that might reflect early mass extinction events that occurred on a planetary scale.     

A Nonlinear Tracker Using Attitude Measurements

The subject of this paper involves tracking the present position of a maneuvering aircraft as well as predicting its future position. A tracking filter is developed that uses aircraft attitude angles (yaw, pitch, roll) in addition to the usual radar measurements. Computer simulation of tracker performance when tracking violently maneuvering aircraft indicates that a dramatic improvement is obtained by using attitude information. The approach taken is to develop a 12-or 15-state extended Kalman filter that models both translational and rotational degrees of freedom. By measuring and estimating attitude it is possible to approximately determine the magnitude and direction of the force system acting on the vehicle and therefore determine vehicle linear acceleration. Knowledge of acceleration is then used to improve the estimate of present and future position of the vehicle being tracked. Simulation of a T-38 aircraft performing a 5 g turn indicates that the new tracker produces maximum trajectory prediction errors that are 36 percent of the errors experienced by more conventional trackers.     

Cave biosignature suites: microbes,minerals, and Mars

Earth's subsurface offers one of the best possible sites to search for microbial life and the characteristic lithologies that life leaves behind. The subterrain may be equally valuable for astrobiology. Where surface conditions are particularly hostile, like on Mars, the subsurface may offer the only habitat for extant lifeforms and access to recognizable biosignatures. We have identified numerous unequivocally biogenic macroscopic, microscopic, and chemical/geochemical cave biosignatures. However, to be especially useful for astrobiology, we are looking for suites of characteristics. Ideally, "biosignature suites" should be both macroscopically and microscopically detectable, independently verifiable by nonmorphological means, and as independent as possible of specific details of life chemistries--demanding (and sometimes conflicting) criteria. Working in fragile, legally protected environments, we developed noninvasive and minimal impact techniques for life and biosignature detection/characterization analogous to Planetary Protection Protocols. Our difficult field conditions have shared limitations common to extraterrestrial robotic and human missions. Thus, the cave/subsurface astrobiology model addresses the most important goals from both scientific and operational points of view. We present details of cave biosignature suites involving manganese and iron oxides, calcite, and sulfur minerals. Suites include morphological fossils, mineral-coated filaments, living microbial mats and preserved biofabrics, 13C and 34S values consistent with microbial metabolism, genetic data, unusual elemental abundances and ratios, and crystallographic mineral forms.     

Interplanetary shocks and sudden impulses during solar maximum (2000) and solar minimum (1995–1996)

In this work a study is performed on the correlation between fast forward interplanetary shock parameters at 1 Astronomical Unit and sudden impulse (SI) amplitudes in the H-component of the geomagnetic field, for periods of solar activity maximum (year 2000) and minimum (year 1995–1996). Solar wind temperature, density and speed, and total magnetic field, were taken to calculate the static pressures (thermal and magnetic) both in the upstream and downstream sides of the shocks. The variations of the solar wind parameters and pressures were then correlated with SI amplitudes. The solar wind speed variations presented good correlations with sudden impulses, with correlation coefficients larger than 0.70 both in solar maximum and solar minimum, whereas the solar wind density presented very low correlation. The parameter better correlated with SI was the square root dynamic pressure variation, showing a larger correlation during solar maximum (r = 0.82) than during solar minimum (r = 0.77). The correlations of SI with square root thermal and magnetic pressure were smaller than with the dynamic pressure, but they also present a good correlation, with r > 0.70 during both solar maximum and minimum. Multiple linear correlation analysis of SI in terms of the three pressure terms have shown that 78% and 85% of the variance in SI during solar maximum and minimum, respectively, are explained by the three pressure variations. Average sudden impulse amplitude was 25 nT during solar maximum and 21 nT during solar minimum, while average square root dynamic pressure variation is 1.20 and 0.86 nPa^1/2 during solar maximum and minimum, respectively. Thus on average, fast forward interplanetary shocks are 33% stronger during solar maximum than during solar minimum, and the magnetospheric SI response has amplitude 20% higher during solar maximum than during solar minimum. A comparison with theoretical predictions (Tsyganenko’s model corrected by Earth’s induced currents) of the coefficient of sudden impulse change with solar wind dynamic pressure variation showed excellent agreement, with values around 17 nT/nPa^1/2.     

Yohkoh observations of the solar corona

The Yohkoh soft X-ray telescope obtains several images every 90 minutes. Data from the declining phase of the solar cycle have been used to compare the X-ray signal with other indicators of activity and to study coronal heating. X-ray emission from a north polar coronal hole is found broadly consistent with results of previous EUV observations. In diffuse emission regions, temperature rises to around 2.2 MK and levels off in the height range 1.5 – 1.9 R_O. Such emission underlies streamers and may be the source of the low-speed solar wind. X-ray signatures for Coronal Mass Ejection (CME) events which involve the detection of reduced X-ray intensities in the corona, have been developed with Yohkoh data. CME observations are described