Cellular responses to gravity: extracellular, intracellular and in-between.

Our understanding of gravitational effects (inertial effects in the vicinity of 1 x g) on cells has matured to a stage at which it is possible to define, on the basis of experimental evidence, extracellular effects on small cells and intracellular effects on eukaryotic gravisensing cells. Yet undetermined is the nature of response, if any, of those classes of cells that are not governed solely by extracellular physical events (as are prokaryotes) and are devoid of obvious mechanical devices for sensing inertial forces (such as those possessed by certain plant cells and sensory cells of animals). This "in-between" class of cells needs to be understood on the basis of the combination of intracellular and extracellular gravity-dependent processes that govern experimentally-measurable variables that are relevant to the cell's responses to modified inertial forces. The forces that certain cell types generate or respond to are therefore compared to those imposed by approximately 1 x g in the context of cytoskeletal action and symmetry-breaking pathways.     

The Effect of Interfering Signals on the Performance of Angle of Arrival Estimates

The performance of angle of arrival estimates using an array in the presence of interfering signals is evaluated using the Cramer-Rao bound. To do this, a model for interference is developed which presents the interference as narrowband, temporally white, but spatially correlated, noise. The bound is evaluated and it is shown to depend upon the ambiguity function of the array and spatial correlation matrix of the noise plus interference. Motivation of the model in the context of air traffic control and sonar surveillance is presented.     

Estimation of Correlation Functions by Stochastic Approximation

Two techniques for estimating the correlation function of a stationary random process using the method of stochastic approximation are developed. Both provide updated estimates as successive T-second observations are processed. Both assume a functional form for the correlation function that depends on a number of parameters that are estimated. One technique is based on the mean-square error; the other maximizes a likelihood function. Examples are included.     

Biologically weighted measurement of UV radiation in space and on Earth with the biofilm technique.

Biological dosimetry has provided experimental proof of the high sensitivity of the biologically effective UVB doses to changes in atmospheric ozone and has thereby confirmed the predictions from model calculations. The biological UV dosimeter 'biofilm' whose sensitivity is based on dried spores of B. subtilis as UV target weights the incident UV radiation according to its DNA damaging potential. Biofilm dosimetry was applicated in space experiments as well as in use in remote areas on Earth. Examples are long-term UV measurements in Antarctica, measurements of diurnal UV profiles parallel in time at different locations in Europe, continuous UV measurements in the frame of the German UV measurement network and personal UV dosimetry. In space biofilms were used to determine the biological efficiency of the extraterrestrial solar UV, to simulate the effects of decreasing ozone concentrations and to determine the interaction of UVB and vitamin D production of cosmonauts in the MIR station.     

A Pulsewidth-Modulated Dc-to-Dc Converter That Utilizes a Small Number of Components

In general, circuit reliability can be enhanced by either incorporating redundancy or by minimizing a circuits component count. Conventional nonregulating converters generally require two active devices, whereas in regulating converters or switching regulators as many as 10 transistors, either integrated or discrete, is not uncommon. A regulating dc-to-dc converter which provides useful regulation and which can be constructed with either two or three transistors is described.     

Determination of parameters of rotational motion of the <Emphasis Type="Italic">Monitor-E</Emphasis> spacecraft from the telemetry data on solar batteries’ current

The Monitor-E spacecraft executed uncontrolled flight due to emergency situation, no telemetry information on parameters of the spacecraft’s attitude motion being available. So, the problem arose to determine the spacecraft’s rotational motion from the accessible indirect information—the electric current provided by solar batteries. In this paper the integrated statistical technique is described, that allows one to solve this problem. The values of current, obtained over the time interval some tens of minutes long, have been processed simultaneously by the least squares method using the integration of the equations of spacecraft’s rotational motion. As a result of processing, the initial conditions of motion were estimated, and the spacecraft’s moments of inertia were updated, as well as the angles, specifying solar batteries position in the spacecraft-fixed coordinate system. The results of processing of 12 data sets are presented, which allowed us to reconstruct the actual rotational motion of the spacecraft.     

Harnessing functional food strategies for the health challenges of space travel—Fermented soy for astronaut nutrition

Astronauts face numerous health challenges during long-duration space missions, including diminished immunity, bone loss and increased risk of radiation-induced carcinogenesis. Changes in the intestinal flora of astronauts may contribute to these problems. Soy-based fermented food products could provide a nutritional strategy to help alleviate these challenges by incorporating beneficial lactic acid bacteria, while reaping the benefits of soy isoflavones. We carried out strain selection for the development of soy ferments, selecting strains of lactic acid bacteria showing the most effective growth and fermentation ability in soy milk (Streptococcus thermophilus ST5, Bifidobacterium longum R0175 and Lactobacillus helveticus R0052). Immunomodulatory bioactivity of selected ferments was assessed using an in vitro challenge system with human intestinal epithelial and macrophage cell lines, and selected ferments show the ability to down-regulate production of the pro-inflammatory cytokine interleukin-8 following challenge with tumour necrosis factor-alpha. The impact of fermentation on vitamin B1 and B6 levels and on isoflavone biotransformation to agluconic forms was also assessed, with strain variation-dependent biotransformation ability detected. Overall this suggests that probiotic bacteria can be successfully utilized to develop soy-based fermented products targeted against health problems associated with long-term space travel.     

Identification of Aircraft Stability and Control Parameters Using Hierarchical State Estimation

Previous attempts to identify aircraft stability and control derivatives from flight test data, using three-degrees-of-freedom (3-DOF) longitudinal or lateral-directional perturbation equation-of-motion models, suffer from the disadvantage that the coupling between the longitudinal and lateral-directional dynamics has been ignored. In this paper, the identification of aircraft stability parameters is accomplished using a more accurate 6-DOF model which includes this coupling. Hierarchical system identification theory is used to reduce the computational effort involved. The 6-DOF system of equations is first decomposed into two 3-DOF subsystems, one for the longitudinal dynamics and the other for the lateral-directional dynamics. The two subsystem parameter identification processes are then coordinated in such a way that the overall system parameter identification problem is solved. Next, a six-subsystem decomposition is considered. Computational considerations and comparison with the unhierarchically structured problem are presented.