Effects of gravity perturbation on developing animal systems

Developing systems provide unique opportunities for analyzing the effects of microgravity on animals. Several unusual types of cells as well as various extraordinary cellular behavior patterns characterize the embryos of most animals. Those features have been exploited as test systems for space flight. The data from previous experiments are reviewed, and considerations for the design of future experiments are presented.     

Distribution of calmodulin in corn seedlings: Immunocytochemical localization in coleoptiles and root apices

Immunofluorescence techniques have been used to study the distribution of calmodulin in several tissues in etiolated corn ( , var. Bear Hybrid) seedlings. Uniform staining was seen in the background cytoplasm of most cell types. Cell walls and vacuoles were not stained. In coleoptile mesophyll cells the nucleoplasm of most nuclei was stained as was the stroma of most amyloplasts. The lumen border of mature tracheary elements in coleoptiles also stained. In the rootcap the most intensely stained regions were the cytoplasms of columella cells and of the outermost cells enmeshed in the layer of secreted slime. Nuclei in the rootcap cells did not stain distinctly, but those in all cell types of the root meristem did. Also in the root meristem, the cytoplasm of metaxylem elements stained brightly. These results are compared and contrasted with previous data on the localization of calmodulin in pea root apices and epicotyls and discussed in relation to current hypotheses on mechanisms of gravitropism.     

Genetic response of bacterial spores to very heavy ions.

Using spores of two Bacillus subtilis strains differing in repair capacity, we have studied repair and mutation induction in the spores after irradiation with very heavy ions up to uranium with specific particle energies up to 18.6 MeV/u. The results indicate that repair and mutation induction after heavy ion irradiation are closely related to each other and that both phenomena strongly depend on the atomic number and specific energy of the ions. The effects are discussed in comparison with results obtained after X-irradiation.     

The Relativistic Proton Spectrometer (RPS) for the Radiation Belt Storm Probes Mission

The Relativistic Proton Spectrometer (RPS) on the Radiation Belt Storm Probes spacecraft is a particle spectrometer designed to measure the flux, angular distribution, and energy spectrum of protons from ～60 MeV to ～2000 MeV. RPS will investigate decades-old questions about the inner Van Allen belt proton environment: a nearby region of space that is relatively unexplored because of the hazards of spacecraft operation there and the difficulties in obtaining accurate proton measurements in an intense penetrating background. RPS is designed to provide the accuracy needed to answer questions about the sources and losses of the inner belt protons and to obtain the measurements required for the next-generation models of trapped protons in the magnetosphere. In addition to detailed information for individual protons, RPS features count rates at a 1-second timescale, internal radiation dosimetry, and information about electrostatic discharge events on the RBSP spacecraft that together will provide new information about space environmental hazards in the Earth’s magnetosphere.     

Overview of QPOs in neutron-star low-mass X-ray binaries

Some aspects of the rapid X-ray variability of low magnetic-field neutron stars in low-mass X-ray binaries are briefly summarized.     

Energetic Hydrogen and Oxygen Atoms Observed on the Nightside of Mars

We present measurements of energetic hydrogen and oxygen atoms (ENAs) on the nightside of Mars detected by the neutral particle detector (NPD) of ASPERA-3 on Mars Express. We focus on the observations for which the field-of-view of NPD was directed at the nightside of Mars or at the region around the limb, thus monitoring the flow of ENAs towards the nightside of the planet. We derive energy spectra and total fluxes, and have compiled maps of hydrogen ENA outflow. The hydrogen ENA intensities reach 10⁵ cm⁻² sr⁻¹ s⁻¹, but no oxygen ENA signals above the detection threshold of 10⁴ cm⁻² sr⁻¹ s⁻¹ are observed. These intensities are considerably lower than most theoretical predictions. We explain the discrepancy as due to an overestimation of the charge-exchange processes in the models for which too high an exospheric density was assumed. Recent UV limb emission measurements (Galli et al., this issue) point to a hydrogen exobase density of 10¹⁰ m⁻³ and a very hot hydrogen component, whereas the models were based on a hydrogen exobase density of 10¹² m⁻³ and a temperature of 200 K predicted by Krasnopolsky and Gladstone (1996). Finally, we estimate the global atmospheric loss rate of hydrogen and oxygen due to the production of ENAs.     

Reconfigurable multiport amplifiers for in-orbit use

A highly reliable and economical system design is presented for a multiport amplifier (MPA) system, which has attracted considerable attention for its potential use in multibeam mobile satellite communications. An MPA is composed of multiple input/output ports and an array of multiple high-power amplifier (HPAs). Since the HPAs are shared among multiple beams, this design solves the problems of traffic imbalance among beams, traffic changes due to terminal movement, and changes in propagation conditions, and can efficiently utilize the total transmitting power with maximum flexibility and minimum hardware. The problem is that HPA failures degrade the MPA beam isolation, causing multipath interference in the surrounding area due to leakage through adjacent beams. To address this problem, we investigated the MPA performance and survival probability when HPAs fail, by taking the specific properties of the MPA configuration into account. Based on our analysis, we found that there is an optimum operational HPA on/off mode in the event of HPA failure, and we were able to obtain optimal performance and reliability by reconfiguring the HPA on/off states in orbit upon the occurrence of HPA failures. Our proposed self-redundant, reconfigurable MPA can achieve high reliability without any need for additional redundant units or switches.     

Near-Earth radiation model deficiencies as seen on CRRES.

The Space Radiation (SPACERAD) experiments on the Combined Release and Radiation Effects Satellite (CRRES) gathered 14 months of radiation particle data in an 18 degrees inclination orbit between 350 km and 36000 km from July 1990 to October 1991. When compared to the NASA radiation belt models AP8 and AE8, the data show the proton model (AP8) does not take into account a second belt formed after major solar flare/shock injection events, and the electron model (AE8) is misleading, at best, in calculating dose in near-Earth orbits. The second proton belt, although softer in energy than the main proton belt, can produce upsets in proton sensitive chips and would produce significant dose in satellites orbiting in it. The MeV electrons observed on CRRES show a significant particle population above 5 MeV (not in the AE8 model) which must be included in any meaningful dose predictions for satellites operating between L-shells of 1.7 and 3.0 RE.     

Solar-interplanetary circumstances associated with the major events in March and June 1991 and comparison with similar events of previous solar cycles.

The solar activity and geomagnetic storm events of March and June 1991 were associated with the appearance of an enhanced particle flux in the trapped radiation belts as discovered by the CRRES satellite observations and later measured by shuttle radiation detectors. The solar-interplanetary conditions associated with these events appear to be a major sequence of activity near the sun's central meridian generating powerful fast interplanetary shocks resulting in major perturbations to the magnetosphere. The solar-interplanetary events in 1991 are discussed and compared to similar activity in the past such as the events in February 1986, August 1972, July 1961, November 1960, and July 1959.