Microorganisms and biomolecules in space environment experiment ES 029 on Spacelab-1.

Bacterial spores are proper test organisms for studying problems of space biology and exobiology. During the Spacelab 1 mission, studies on the limiting factors for survival of Bacillus subtilis spores in free space have been performed. An exposure tray on the pallet of Spacelab 1 accomodated 316 samples of dry spores for treatment with space vacuum and/or the following selected wavelengths of solar UV: > 170 nm, 220 nm, 240nm, 260nm and 280 nm. After recovery, inactivation, mutation induction, reparability, and photochemical damages in DNA and protein have been studied. The results contribute to the understanding of the mechanisms of increased UV sensitivity of bacterial spores in vacuo and to a better assessment of the chance of survival of resistant forms in space and of interplanetary transfer of life.     

The organic aerosols of Titan

A dark reddish organic solid, called tholin, is synthesized from simulated Titanian atmospheres by irradiation with high energy electrons in a plasma discharge. The visible reflection spectrum of this tholin is found to be similar to that of high altitude aerosols responsible for the albedo and reddish color of Titan. The real (n) and imaginary (k) parts of the complex refractive index of thin films of Titan tholin prepared by continuous D.C. discharge through a 0.9 N₂/0.1 CH₄ gas mixture at 0.2 mb is determined from x-ray to microwave frequencies. Values of n (1.65) and k (0.004 to 0.08) in the visible are consistent with deductions made by ground-based and spaceborne observations of Titan. Many infrared absorption features are present in k(λ), including the 4.6 μm nitrile band. Molecular analysis of the volatile component of this tholin was performed by sequential and non-sequential pyrolytic gas chromatography/mass spectrometry. More than one hundred organic compounds are released; tentative identifications include saturated and unsaturated aliphatic hydrocarbons, substituted polycyclic aromatics, nitriles, amines, pyrroles, pyrazines, pyridines, pyrimidines, and the purine, adenine. In addition, acid hydrolysis produces a racemic mixture of biological and non-biological amino acids. Many of these molecules are implicated in the origin of life on Earth, suggesting Titan as a contemporary laboratory environment for prebiological organic chemistry on a planetary scale.     

Flare build up: 21 May 1980

The decaying solar active region that crossed the central meridian on May 20, 1980 at latitude S13° produced a major flare (2B/X1) at 2054 on May 21. This region was a target of the international Flare Buildup Study and was well observed. The buildup was characterized by little flare activity during two days prior to the major flare but a great deal of activity in the filament that separated the opposite magnetic polarities of the active region. Large proper motions of sunspots and magnetic fields suggest that the magnetic field was stressed prior to the flare. The immediate trigger of the flare appears to have been an eruption of new magnetic flux in the center of the active region. The new flux erupted in a configuration that decreased the net flux of the active region and contributed to the decay of the region.     

Localization performance of arrays subject to phase errors

The passive localisation of radiating sources using an array subject to random perturbations in sensor phases is presented. All source signals as well as additive noises observed at the sensors are assumed to be independent identically distributed zero-mean Gaussian random processes. Cramer-Rao bounds are derived for source bearings and ranges for the phase errors at each sensor. It is shown that accurate phase calibration can be achieved when the number of sources exceeds a certain minimum. The locations of the calibrating sources need not be known a priori and need only satisfy mild regularity conditions. A calibration procedure is proposed which uses maximum-likelihood techniques     

Forces acting on a foreign particle near a solidification front

The interaction of inert foreign particles and a solid-liquid interface during solidification is considered. The viscous drag on the particle which produces a force on the interface is calculated under general assumptions as well as the disjoining force for small particles and the buoyancy force for large ones.     

Computational fluid dynamics and material processing (buoyancy and thermocapillary convections in melts during directional crystallization)

Modelisation and solution of heat and mass transfer problems relevant for material processing are generally hard to be handled, as they often involve 3D unsteady flows, viscous mixtures, phase changes, moving liquid-solid fronts, deforming liquid-gas interfaces, etc.… For space applications, material processing benefits of reduced buoyancy convection but can be faced to a strongly increased complexity due to variable g, mainly in manned flight.

Computational techniques used to analyse fluid motions in material processing, accounting for free surface, crystallization front and bulk convection in melt, are reviewed with emphasis to directional crystallization. Hydrodynamics stability and bifurcation analysis are shown to be useful complementary tools for correlating data, and for a better understanding of the physical laws. This last point will be illustrated in the case of the onset of oscillations in metallic melts.     

Identification of specific gravity sensitive signal transduction pathways in human A431 carcinoma cells.

Epidermal growth factor (EGF) activates a well characterized signal transduction cascade in human A431 epidermoid carcinoma cells. The influence of gravity on EGF-induced EGF-receptor clustering and early gene expression as well as on actin polymerization and actin organization have been investigated. Different signalling pathways induced by the agents TPA, forskolin and A23187 that activate gene expression were tested for sensitivity to gravity. EGF-induced c-fos and c-jun expression were decreased in microgravity. However, constitutive beta-2 microglobulin expression remained unaltered. Under simulated weightlessness conditions EGF- and TPA-induced c-fos expression was decreased, while forskolin- and A23187-induced c-fos expression was independent of the gravity conditions. These results suggest that gravity affects specific signalling pathways. Preliminary results indicate the EGF-induced EGF-receptor clustering remained unaltered irrespective of the gravity conditions. Furthermore, the relative filamentous actin content of steady state A431 cells was enhanced under microgravity conditions and actin filament organization was altered. Under simulated weightlessness actin filament organization in steady state cells as well as in EGF-treated cells was altered as compared to the 1 G reference experiment. Interestingly the microtubule and keratin organization in untreated cells showed no difference with the normal gravity samples. This indicates that gravity may affect specific components of the signal transduction circuitry.     

Three Mile Island? Lessons for America

The great fear of a nuclear castastrophe is the prime lesson learned from the Three Mile Island accident. This fear is real and creates a major risk to our society. Although the probability of a major release of radioactivity occurring at a given plant in a given year is low, the rapidly increasin number of plants around the world increases the probability that such a catastrophe will occur someplace in the next few years. When a major release does occur, people will demand an end to the use of energy from nuclear power. We are at grave risk in not having an alternative, in not providing a choice in time. The alternatives are clearly defined. But we need to see that the resources are made available to develop them.