Fluorescence microscopy as a tool for in situ life detection

The identification of extant and, in some cases, extinct bacterial life is most convincingly and efficiently performed with modern high-resolution microscopy. Epifluorescence microscopy of microbial autofluorescence or in conjunction with fluorescent dyes is among the most useful of these techniques. We explored fluorescent labeling and imaging of bacteria in rock and soil in the context of in situ life detection for planetary exploration. The goals were two-fold: to target non-Earth-centric biosignatures with the greatest possible sensitivity and to develop labeling procedures amenable to robotic implementation with technologies that are currently space qualified. A wide panel of commercially available dyes that target specific biosignature molecules was screened, and those with desirable properties (i.e., minimal binding to minerals, strong autofluorescence contrast, no need for wash steps) were identified. We also explored the potential of semiconductor quantum dots (QDs) as bacterial and space probes. A specific instrument for space implementation is suggested and discussed.     

Some ecological mechanisms to generate habitability in planetary subsurface areas by chemolithotrophic communities: the Río Tinto subsurface ecosystem as a model system

Chemolithotrophic communities that colonize subsurface habitats have great relevance for the astrobiological exploration of our Solar System. We hypothesize that the chemical and thermal stabilization of an environment through microbial activity could make a given planetary region habitable. The MARTE project ground-truth drilling campaigns that sampled cryptic subsurface microbial communities in the basement of the Río Tinto headwaters have shown that acidic surficial habitats are the result of the microbial oxidation of pyritic ores. The oxidation process is exothermic and releases heat under both aerobic and anaerobic conditions. These microbial communities can maintain the subsurface habitat temperature through storage heat if the subsurface temperature does not exceed their maximum growth temperature. In the acidic solutions of the Río Tinto, ferric iron acts as an effective buffer for controlling water pH. Under anaerobic conditions, ferric iron is the oxidant used by microbes to decompose pyrite through the production of sulfate, ferrous iron, and protons. The integration between the physical and chemical processes mediated by microorganisms with those driven by the local geology and hydrology have led us to hypothesize that thermal and chemical regulation mechanisms exist in this environment and that these homeostatic mechanisms could play an essential role in creating habitable areas for other types of microorganisms. Therefore, searching for the physicochemical expression of extinct and extant homeostatic mechanisms through physical and chemical anomalies in the Mars crust (i.e., local thermal gradient or high concentration of unusual products such as ferric sulfates precipitated out from acidic solutions produced by hypothetical microbial communities) could be a first step in the search for biological traces of a putative extant or extinct Mars biosphere.     

The Dynamic Albedo of Neutrons (DAN) experiment for NASA's 2009 Mars Science Laboratory

We present a summary of the physical principles and design of the Dynamic Albedo of Neutrons (DAN) instrument onboard NASA's 2009 Mars Science Laboratory (MSL) mission. The DAN instrument will use the method of neutron-neutron activation analysis in a space application to study the abundance and depth distribution of water in the martian subsurface along the path of the MSL rover.     

The Physical Foundation of Forecasting Disturbances in the Geospace From Solar Disk Characteristics

The five main types of antisunward propagating energetic fluxes (particles and emission) may be thought of as well established to date, the effects of which lead to a particilar character of disturbance in the near-terrestrial environment (the Earth's magnetosphere, ionosphere and atmosphere). The strongest global restructuring of the magnetosphere and ionosphere is caused by fluxes of relatively dense n of 1-70 cm-3 at the Earth's orbit) Solar Wind (SW) quasi-neutral, low-energy (E ＜ 10 keV) plasma which cause magnetospheric and ionospheric storms lasting 24 hours or longer. For that reason, main attention is given to their study at the initial stage of research. The physical essence of the method of predicting disturbances in the near-terrestrial space environment, the amplitude of which can be expressed in, for example, the Kp index units, involves:(1) identifying all the most geo-effective SW streams of type, (2) determing their sources on the solar disk,and (3) quantifying the correlations between the characteristics of their solar sources with a maximum value of the Kp-index that is caused by the concerned type of SW stream. Semi-phenomenological relations have been obtained, which relate parameters of type SW stream sources to characteristics of geomagnetic storms:storm commencement, the time at which the storm intensity reaches its maximum values, the storm duration,as well as to the storm amplitude expressed in terms of geomagnetic indeces.      

Transport modeling of energetic electrons in the inner magnetosphere with synchrotron energy losses

The resulting L-distributions and energy spectra of energetic magnetospheric electrons obtained from numerical solution of the radiation belt transport equation with and without accounting for electron synchrotron energy losses are compared. It is demonstrated that synchrotron losses play an important role in formation of the space and energetic distributions of electrons in the inner magnetosphere.     

Substorms in the inner plasma sheet

Thin Current Sheets (TCS) are regularly formed prior to substorm breakup, even in the near-Earth plasma sheet, as close as the geostationary orbit. A self-consistent kinetic theory describing the response of the plasma sheet to an electromagnetic perturbation is given. This perturbation corresponds to an external forcing, for instance caused by the solar wind (not an internal instability). The equilibrium of the configuration of this TCS in the presence of a time varying perturbation is shown to produce a strong parallel thermal anisotropy (T T) of energetic electrons and ions (E>50keV) as well as an enhanced diamagnetic current carried by low energy ions (E<50keV). Both currents tend to enhance the confinement of this current sheet near the magnetic equator. These results are compared with data gathered by GEOS-2 at the geostationary orbit, where the magnetic signatures of TCS, and parallel anisotropics are regularly observed prior to breakup. By ensuring quasi-neutrality everywhere we find, when low frequency electromagnetic perturbations are applied, that although the magnetic field line remains an equipotential to the lowest order in T_e/T_i, a field-aligned potential drop exists to the next order in (T_e/T_i). Thus the development of a TCS implies the formation of a field-aligned potential drop ( few hundred volts) to ensure the quasi-neutrality everywhere. For an earthward directed pressure gradient, a field-aligned electric field, directed towards the ionosphere, is obtained, on the western edge of the perturbation (i.e. western edge of the current sheet). Thus field aligned beams of electrons are expected to flow towards the equatorial region on the western edge of the current sheet. We study the stability of these electron beams and show that they are unstable to “High Frequency” (HF) waves. These “HF” waves are regularly observed at frequencies of the order of the proton gyrofrequency (f_H+) just before, or at breakup. The amplitude of these HF waves is so large that they can produce a strong pitch-angle diffusion of energetic ions and a spatial diffusion that leads to a reduction of the diamagnetic current. The signature of a fast ion diffusion is indeed regularly observed during the early breakup; it coincides with the sudden development of large amplitude transient fluctuations, ballooning modes, observed at much lower frequencies (fH+). These results suggest that the HF waves, generated by field-aligned electron beams, provide the dissipation which is necessary to destabilize low frequency (ballooning) modes.     

Radio plasma imager simulations and measurements

The Radio Plasma Imager (RPI) will be the first-of-its kind instrument designed to use radio wave sounding techniques to perform repetitive remote sensing measurements of electron number density (N _e) structures and the dynamics of the magnetosphere and plasmasphere. RPI will fly on the Imager for Magnetopause-to-Aurora Global Exploration (IMAGE) mission to be launched early in the year 2000. The design of the RPI is based on recent advances in radio transmitter and receiver design and modern digital processing techniques perfected for ground-based ionospheric sounding over the last two decades. Free-space electromagnetic waves transmitted by the RPI located in the low-density magnetospheric cavity will be reflected at distant plasma cutoffs. The location and characteristics of the plasma at those remote reflection points can then be derived from measurements of the echo amplitude, phase, delay time, frequency, polarization, Doppler shift, and echo direction. The 500 m tip-to-tip X and Y (spin plane) antennas and 20 m Z axis antenna on RPI will be used to measures echoes coming from distances of several R _E. RPI will operate at frequencies between 3 kHz to 3 MHz and will provide quantitative N _e values from 10^–1 to 10⁵ cm^–3. Ray tracing calculations, combined with specific radio imager instrument characteristics, enables simulations of RPI measurements. These simulations have been performed throughout an IMAGE orbit and under different model magnetospheric conditions. They dramatically show that radio sounding can be used quite successfully to measure a wealth of magnetospheric phenomena such as magnetopause boundary motions and plasmapause dynamics. The radio imaging technique will provide a truly exciting opportunity to study global magnetospheric dynamics in a way that was never before possible.     

The Radio Plasma Imager investigation on the IMAGE spacecraft

Radio plasma imaging uses total reflection of electromagnetic waves from plasmas whose plasma frequencies equal the radio sounding frequency and whose electron density gradients are parallel to the wave normals. The Radio Plasma Imager (RPI) has two orthogonal 500-m long dipole antennas in the spin plane for near omni-directional transmission. The third antenna is a 20-m dipole along the spin axis. Echoes from the magnetopause, plasmasphere and cusp will be received with the three orthogonal antennas, allowing the determination of their angle-of-arrival. Thus it will be possible to create image fragments of the reflecting density structures. The instrument can execute a large variety of programmable measuring options at frequencies between 3 kHz and 3 MHz. Tuning of the transmit antennas provides optimum power transfer from the 10 W transmitter to the antennas. The instrument can operate in three active sounding modes: (1) remote sounding to probe magnetospheric boundaries, (2) local (relaxation) sounding to probe the local plasma frequency and scalar magnetic field, and (3) whistler stimulation sounding. In addition, there is a passive mode to record natural emissions, and to determine the local electron density, the scalar magnetic field, and temperature by using a thermal noise spectroscopy technique.     

Effect of non-uniform basic temperature gradient on the onset of Marangoni convection in a fluid with suspended particles

The effect of a non-uniform basic temperature gradient on the onset of convection driven by surface tension in a horizontal layer of a Boussinesq fluid with suspended particles confined between an upper free, constant heat flux boundary and a lower rigid isothermal boundary is considered. The microrotation is assumed to vanish at the boundaries. A linear stability analysis is performed. The Rayleigh–Ritz technique is used to obtain the eigenvalues. The influence of various parameters on the onset of convection has been analyzed. Six different non-uniform basic state temperature profiles are considered and their comparative influence on onset is discussed. It is observed that the fluid layer with suspended particles heated from below is more stable compared to the classical fluid layer without suspended particles. The problem has possible applications in microgravity situations.