Radial and meridional trends in solar wind thermal electron temperature and anisotropy: Ulysses

Ulysses plasma measurement from 1.15 to 5.31 AU and from S6.4° to S48.3° solar latitude are used to assess the trends in the solar wind thermal electron temperature and anisotropy. Improved spacecraft potential corrections and data products have been incorporated. The radial temperature gradient is steeper than in previous determinations, but flatter than adiabatic. When normalized to 1 AU, temperature decrease with increasing latitude. Little change in the average thermal anisotropy has been seen during the mission.     

Solar wind corotating stream interaction regions out of the ecliptic plane: Ulysses

Ulysses plasma observations reveal that the forward shocks that commonly bound the leading edges of corotating interaction regions (CIRs) beyond 2 AU from the Sun at low heliographic latitudes nearly disappeared at a latitude of S26°. On the other hand, the reverse shocks that commonly bound the trailing edges of the CIRs were observed regularly up to S41.5°, but became weaker with increasing latitude. Only three CIR shocks have been observed poleward of S41.5°; all of these were weak reverse shocks. The above effects are a result of the forward waves propagating to lower heliographic latitudes and the reverse waves to higher latitudes with increasing heliocentric distance. These observational results are in excellent agreement with the predictions of a global model of solar wind flows that originate in a simple tilted-dipole geometry back at the Sun.     

A three-dimensional plasma and energetic particle investigation for the wind spacecraft

This instrument is designed to make measurements of the full three-dimensional distribution of suprathermal electrons and ions from solar wind plasma to low energy cosmic rays, with high sensitivity, wide dynamic range, good energy and angular resolution, and high time resolution. The primary scientific goals are to explore the suprathermal particle population between the solar wind and low energy cosmic rays, to study particle accleration and transport and wave-particle interactions, and to monitor particle input to and output from the Earth's magnetosphere.Three arrays, each consisting of a pair of double-ended semi-conductor telescopes each with two or three closely sandwiched passivated ion implanted silicon detectors, measure electrons and ions above 20 keV. One side of each telescope is covered with a thin foil which absorbs ions below 400 keV, while on the other side the incoming <400 keV electrons are swept away by a magnet so electrons and ions are cleanly separated. Higher energy electrons (up to 1 MeV) and ions (up to 11 MeV) are identified by the two double-ended telescopes which have a third detector. The telescopes provide energy resolution of E/E0.3 and angular resolution of 22.5°×36°, and full 4 steradian coverage in one spin (3 s).Top-hat symmetrical spherical section electrostatic analyzers with microchannel plate detectors are used to measure ions and electrons from 3 eV to 30 keV. All these analyzers have either 180° or 360° fields of view in a plane, E/E0.2, and angular resolution varying from 5.6° (near the ecliptic) to 22.5°. Full 4 steradian coverage can be obtained in one-half or one spin. A large and a small geometric factor analyzer measure ions over the wide flux range from quiet-time suprathermal levels to intense solar wind fluxes. Similarly two analyzers are used to cover the wide range of electron fluxes. Moments of the electron and ion distributions are computed on board.In addition, a Fast Particle Correlator combines electron data from the high sensitivity electron analyzer with plasma wave data from the WAVE experiment (Bougeretet al., in this volume) to study wave-particle interactions on fast time scales. The large geometric factor electron analyzer has electrostatic deflectors to steer the field of view and follow the magnetic field to enhance the correlation measurements.     

The multifuid solar wind termination shock and its influence on the threedimensional plasma structure upstream and downstream

The solar wind termination shock is described as a multi-fluid phenomenon taking into account the magnetohydrodynamic self-interaction of a multispecies plasma consisting of solar wind ions, pick-up ions and shock-generated anomalous cosmic ray particles. The spatial diffusion of these high energy particles relative to the resulting, pressure-modified solar wind flow structure is described by a coupled system of differential equations describing mass-, momentum-, and energy-flow continuities for all plasma components. The energy loss due to escape of energetic particles (MeV) from the precursor into the inner heliosphere is taken into account. We determine the integrated properties of the anomalous cosmic ray gas and the low-energy solar wind. Also the variation of the compression ratio of the shock structure is quantitatively determined and is related to the pick-up ion energization efficiency and to the mean energy of the downstream anomalous cosmic ray particles. The variation of the resulting shock structure and of the solar wind sheath plasma extent beyond the shock is discussed with respect to its consequences for the LISM neutral gas filtration and the threedimensional shape of the heliosphere.     

The Plasma Environment of Comet 67P/Churyumov-Gerasimenko Throughout the Rosetta Main Mission

The plasma environment of comet 67P/Churyumov-Gerasimenko, the Rosetta mission target comet, is explored over a range of heliocentric distances throughout the mission: 3.25 AU (Rosetta instruments on), 2.7 AU (Lander down), 2.0 AU, and 1.3 AU (perihelion). Because of the large range of gas production rates, we have used both a fluid-based magnetohydrodynamic (MHD) model as well as a semi-kinetic hybrid particle model to study the plasma distribution. We describe the variation in plasma environs over the mission as well as the differences between the two modeling approaches under different conditions. In addition, we present results from a field aligned, two-stream transport electron model of the suprathermal electron flux when the comet is near perihelion.     

Design of a Correlator for Real-Time Video Comparisons

The problem of locating a reference image within a larger image using a correlation technique is discussed. Although the particular application discussed is that of locating a reference image obtained from one video sensor or a photograph, within the larger field of view obtained from a second video sensor in real time (i.e., at the TV field rate), the results are general and useful for a number of applications. The tradeoffs necessary to obtain real time correlat are discussed and their effect on correlation accuracy is given.     

Early development of fern gametophytes in microgravity.

Dormant spores of the fern Ceratopteris richardii were flown on Shuttle mission STS-93 to evaluate the effects of micro-g on their development and on their pattern of gene expression. Prior to flight the spores were sterilized and sown into one of two environments: (1) Microscope slides in a video-microscopy module; and (2) Petri dishes. All spores were then stored in darkness until use. Spore germination was initiated on orbit after exposure to light. For the spores on microscope slides, cell level changes were recorded through the clear spore coat of the spores by video microscopy. After their exposure to light, spores in petri dishes were frozen in orbit at four different time points during which on earth gravity fixes the polarity of their development. Spores were then stored frozen in Biological Research in Canister units until recovery on earth. The RNAs from these cells and from 1-g control cells were extracted and analyzed on earth after flight to assay changes in gene expression. Video microscopy results revealed that the germinated spores developed normally in microgravity, although the polarity of their development, which is guided by gravity on earth, was random in space. Differential Display-PCR analyses of RNA extracted from space-flown cells showed that there was about a 5% change in the pattern of gene expression between cells developing in micro-g compared to those developing on earth.     

Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation.

The lens epithelium is the initiation site for the development of radiation induced cataracts. Radiation in the cortex and nucleus interacts with proteins, while in the epithelium, experimental results reveal mutagenic and cytotoxic effects. It is suggested that incorrectly repaired DNA damage may be lethal in terms of cellular reproduction and also may initiate the development of mutations or transformations in surviving cells. The occurrence of such genetically modified cells may lead to lens opacification. For a quantitative risk estimation for astronauts and space travelers it is necessary to know the relative biological effectiveness (RBE), because the spacial and temporal distribution of initial physical damage induced by cosmic radiation differ significantly from that of X-rays. RBEs for the induction of DNA strand breaks and the efficiency of repair of these breaks were measured in cultured diploid bovine lens epithelial cells exposed to different LET irradiation to either 300 kV X-rays or to heavy ions at the UNILAC accelerator at GSI. Accelerated ions from Z=8 (O) to Z=92 (U) were used. Strand breaks were measured by hydroxyapatite chromatography of alkaline unwound DNA (overall strand breaks). Results showed that DNA damage occurs as a function of dose, of kinetic energy and of LET. For particles having the same LET the severity of the DNA damage increases with dose. For a given particle dose, as the LET rises, the numbers of DNA strand breaks increase to a maximum and then reach a plateau or decrease. Repair kinetics depend on the fluence (irradiation dose). At any LET value, repair is much slower after heavy ion exposure than after X-irradiation. For ions with an LET of less than 10,000 keV micrometers-1 more than 90 percent of the strand breaks induced are repaired within 24 hours. At higher particle fluences, especially for low energetic particles with a very high local density of energy deposition within the particle track, a higher proportion of non-rejoined breaks is found, even after prolonged periods of incubation. At the highest LET value (16,300 keV micrometers-1) no significant repair is observed. These LET-dependencies are consistent with the current mechanistic model for radiation induced cataractogenesis which postulates that genomic damage to the surviving fraction of epithelial cells is responsible for lens opacification.     

The detection of energetic cometary and solar particles by the EPONA instrument on the Giotto mission

EPONA is an energetic particle detector system incorporating totally depleted silicon surface barrier layer detectors. Active and passive background shielding will be employed and, by applying various techniques, particles of different species, including electrons, protons, alpha particles and pick-up ions of cometary origin may be detected over a wide spectrum of energies extending from the tens of KeV into the MeV range.

The instrument can operate in two modes namely (a) in a cruise phase or storage mode and (b) in a real time mode. During the real time mode, observations at high spatial (octosectoring) and temporal (0.5s) resolution in the cometary environment permit studies to be made of accelerated particles at the bow shock and/or in the tail of the comet. In conjunction with magnetic field measurements on board Giotto, observations of energetic electrons and their anisotropies can determine whether the magnetic field lines in the cometary tail are open or closed. Further, the absorption of low energy solar particles in the cometary atmosphere can be measured and such data would provide an integral value of the pertaining gas and dust distribution. Solar particle background measurements during encounter may also be used to correct the measurements of other spacecraft borne instruments potentially vulnerable to such radiation.

Solar particle flux measurements, obtained during the cruise phase will, when combined with simultaneous observations made by other spacecraft at different heliographic longitudes, provide information concerning solar particle propagation in the corona and in interplanetary space.     

Charged-particle mutagenesis II. Mutagenic effects of high energy charged particles in normal human fibroblasts.

The biological effects of high LET charged particles are a subject of great concern with regard to the prediction of radiation risk in space. In this report, mutagenic effects of high LET charged particles are quantitatively measured using primary cultures of human skin fibroblasts, and the spectrum of induced mutations are analyzed. The LET of the charged particles ranged from 25 KeV/micrometer to 975 KeV/micrometer with particle energy (on the cells) between 94-603 MeV/u. The X-chromosome linked hypoxanthine guanine phosphoribosyl transferase (hprt) locus was used as the target gene. Exposure to these high LET charged particles resulted in exponential survival curves; whereas, mutation induction was fitted by a linear model. The Relative Biological Effect (RBE) for cell-killing ranged from 3.73 to 1.25, while that for mutant induction ranged from 5.74 to 0.48. Maximum RBE values were obtained at the LET of 150 keV/micrometer. The inactivation cross-section (alpha i) and the action cross-section for mutant induction (alpha m) ranged from 2.2 to 92.0 micrometer2 and 0.09 to 5.56 x 10(-3) micrometer2, respectively. The maximum values were obtained by 56Fe with an LET of 200 keV/micrometer. The mutagenicity (alpha m/alpha i) ranged from 2.05 to 7.99 x 10(-5) with the maximum value at 150 keV/micrometer. Furthermore, molecular analysis of mutants induced by charged particles indicates that higher LET beams are more likely to cause larger deletions in the hprt locus.