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951.
Activity of auxin polar transport in inflorescence axes of Arabidopsis thaliana grown under simulated microgravity conditions was studied in relation to the growth and development. Seeds were germinated and allowed to grow on an agar medium in test tubes on a horizontal clinostat. Horizontal clinostat rotation substantially reduced the growth of inflorescence axes and the productivity of seeds of Arabidopsis thaliana (ecotypes Landsberg erecta and Columbia), although it little affected seed germination, development of rosette leaves and flowering. The activity of auxin polar transport in inflorescence axes decreased when Arabidopsis plants were grown on a horizontal clinostat from germination stage, being ca. 60% of 1 g control. On the other hand, the auxin polar transport in inflorescence axes of Arabidopsis grown in 1 g conditions was not affected when the segments were exposed to various gravistimuli, including 3-dimensional clinorotation, during transport experiments. Pin-formed mutant of Arabidopsis, having a unique structure of the inflorescence axis with no flower and extremely low levels of the activity of auxin polar transport in inflorescence axes and endogenous auxin, did not continue its vegetative growth under clinostat rotation. These facts suggest that the development of the system of auxin polar transport in Arabidopsis is affected by microgravity, resulting in the inhibition of growth and development, especially during reproductive growth.  相似文献   
952.
The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.  相似文献   
953.
The aim of the proposed Beagle 2 small lander for ESA's 2003 Mars Express mission is to search for organic material on and below the surface of Mars and to study the inorganic chemistry and mineralogy of the landing site. The lander will have a total mass of 60kg including entry, descent, and landing system. Experiments will be deployed on the surface using a robotic arm. It will use a mechanical mole and grinder to obtain samples from below the surface, under rocks, and inside rocks. Sample analysis by a mass spectrometer will include isotopic analysis. An optical microscope, an X-ray spectrometer and a Mossbauer spectrometer will conduct in-situ rock studies.  相似文献   
954.
A novel sparse array geometry embedding two sizes of spatial invariances is presented for use with a new ESPRIT-based (estimation of signal parameters via rotational invariance techniques) algorithm for aperture extension. The half-wavelength invariance yields unambiguous but high-variance direction cosine estimates to disambiguate low-variance but cyclically ambiguous estimates from the larger invariance. With larger invariance at 60 half-wavelengths, resolution threshold for two closely spaced emitters is reduced by 50 dB and estimation error by 100-fold. Array design formulas are also presented  相似文献   
955.
Results of a numerical (CFD) study of the influence of the forebody shape on local flow parameters at a bottom-mounted inlet entrance are presented. The free-stream Mach number is assumed to be 3.5–7.0. Some recommendations on forebody shape optimization are provided. Main characteristics of the air inlet are evaluated.  相似文献   
956.
Residual chromatin breaks as biodosimetry for cell killing by carbon ions   总被引:5,自引:0,他引:5  
We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET= 13 keV/μm, 76 keV/μm) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour postirradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/μm beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.  相似文献   
957.
用CBN刀具高速切削灰铸铁的试验研究   总被引:2,自引:0,他引:2  
研究了CBN刀具高速切削灰铸铁时的刀具前、后刀面磨损、耐用度以及富氧气氛对切削性能的影响,所用的灰铸铁具有不同的铁素体、珠光体比例,最高切削速度达1'100 m/min.研究表明Amborite刀具的刀尖磨损略大于BZN6000刀具,在较低速度下加工以铁素体为主的灰铸铁时更容易发生月牙洼磨损;BZN6000刀具以700 m/min的切削速度加工铁素体含量较高的灰铸铁时耐用度较高,Amborite刀具的耐用度随切削速度而升高;在富氧气氛中切削可以减轻刀具磨损,这一效果对BZN6000刀具更为显著.文章中对这些现象的原因进行了讨论.  相似文献   
958.
In the 18.5-day flight of the Soviet biosatellite Cosmos-936 (3-22, August 1977) com-parative investigations of the physiological effects of prolonged weightlessness (20 rats) and artificial gravity of 1 g (10 rats) were carried out. Throughout the flight artificial gravity was generated by means of animal rotation in two centrifuges with a radius of 320mm. Postflight examination of animals and treatment of the flight data were performed by Soviet scientists in collaboration with the specialists from Bulgaria, Czechoslovakia, the German Democratic Republic, Hungary, Poland, Rumania, France and the U.S.A. During the flight the total motor activity of the weightless rats was higher and their body temperature was lower than those of the centrifuged animals. Postflight examination of the weightless rats showed a greater percentage of errors during maze an increase in water intake and a decrease in diuresis; a fall of the resistance of peripheral red cells; an increase in the conditionally pathogenic microflora in the mouth; a decrease of oxygen consumption, carbon dioxide production and energy expenditures; a drop in the static physical endurance; a decline in the capacity to keep balance on the rail; an increase in the latent period of the lifting reflex, etc. The centrifugal animals displayed lesser or no change of the above type. These findings together with the biochemical and morphological data give evidence that during and after flight adaptive processes in the centrifuged rats developed better.  相似文献   
959.
A method of technologic audit for technical re-equipment of enterprises is considered. The method developed is intended to verify technological solutions of projects of technical re-equipment of enterprises according to indicators of productivity.  相似文献   
960.
We developed two types of hybrid terminals that can provide both satellite communication and position determination services in one system. One terminal uses the single channel per carrier (SCPC) technique and the other uses the spread spectrum (SS) technique. To evaluate the performance of the two systems, we carried out experiments in Japan and in the Pacific Ocean using two geostationary satellites, ETS-V (150°E) and Inmarsat (180°E). The ranging accuracy between the mobile terminals and the base station via the satellites was found to be about 200 m using the SCPC system and about 10 m using the SS system. The measured positioning accuracy was about 1 km in the SCPC system and about 600 m in the SS system when experiments were carried out near Japan. The experimental results show that the positioning errors were mainly caused by the orbital determination errors of the two satellites. Presented here are the configurations and features of the SCPC and SS terminals, the experimental system, and the experimental results  相似文献   
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