Paramecium--a model system for studying cellular graviperception.

Experiments under varied gravitational accelerations as well as in density-adjusted media showed that sensation of gravity in protists may be linked to the known principles of mechanosensation. Paramecium, a ciliate with clear graviresponses (gravitaxis and gravikinesis) is an ideal model system to prove this hypothesis since the ciliary activity and thus the swimming behaviour is controlled by the membrane potential. It has also been assumed that the cytoplasmic mass causes a distinct stimulation of the bipolarly distributed mechano-sensitive K+ and Ca2+ ion channels in the plasma membrane in dependence of the spatial orientation of the cell. In order to prove this hypothesis, different channel blockers are currently under investigation. Gadolinium did not inhibit gravitaxis in Paramecium, showing that it does not specifically block gravireceptors. Further studies concentrated on the question of whether second messengers are involved in the gravity signal transduction chain. Exposure to 5 g for up to 10 min led to a significant increase in cAMP.     

A bioreactor system for the nitrogen loop in a Controlled Ecological Life Support System.

As space missions become longer in duration, the need to recycle waste into useful compounds rises dramatically. This problem can be addressed by the development of Controlled Ecological Life Support Systems (CELSS) (i.e., Engineered Closed/Controlled Eco-Systems (ECCES)), consisting of human and plant modules. One of the waste streams leaving the human module is urine. In addition to the reclamation of water from urine, recovery of the nitrogen is important because it is an essential nutrient for the plant module. A 3-step biological process for the recycling of nitrogenous waste (urea) is proposed. A packed-bed bioreactor system for this purpose was modeled, and the issues of reaction step segregation, reactor type and volume, support particle size, and pressure drop were addressed. Based on minimization of volume, a bioreactor system consisting of a plug flow immobilized urease reactor, a completely mixed flow immobilized cell reactor to convert ammonia to nitrite, and a plug flow immobilized cell reactor to produce nitrate from nitrite is recommended. It is apparent that this 3-step bioprocess meets the requirements for space applications.     

GPS based onboard and onground orbit operations for small satellites

In extension to common applications such as groundtrack displays and antenna steering, the SGP4 orbit model is proposed for operational orbit determination in small satellite missions. SGP4 is an analytical orbit model for Low-Earth orbiting satellites that is widely used for the propagation of NORAD twoline elements. Twoline elements may hence be generated completely independent of NORAD. Their use as exclusive source of orbital information simplifies the operations concept and reduces mission costs through the extensive use of existing low-cost mission support software. Due to small computer resource requirements of 8–10kByte, the SGP4 model may also be applied for onboard orbit computations making use of e.g. a 80186 processor, thus ensuring full compatibility of ground-based and onboard operations. The proposed approach is particularly suited in combination with a space-borne GPS receiver, were the C/A-code navigation solutions are treated as measurements that are adjusted in a least-squares sense using the SGP4 model. As consequence, inherent drawbacks of the pure navigation solutions such as data gaps and scatter as well as limited velocity accuracy are avoided, while the operational navigation activities are kept at a minimum. The feasibility of the concept is illustrated based on real GPS navigation data from the TOPEX/Poseidon and the MIR space station with an inherent data quality of 50–100 m. It is shown that 3 hours of data within a 4 day period are sufficient to keep the position error within 4 km, that is considered sufficient for most applications.     

Century scale solar variability imprinted in the 44Ti activity in meteorites.

The measurements of gamma-activity of the 44Ti (T1/2 = 66.6 years) produced by spallation reaction of galactic cosmic rays (GCR) in Alfianello, Olivenza, Rio Negro, Dhajala and Torino meteorites, which fell in the time interval 1883 AD (Alfianello)-1988 AD (Torino), show a century scale modulation, connected to long-term solar-wind flux variations in the interplanetary space. The variation of the 44Ti activity with the time of fall of meteorites is qualitatively consistent with the Gleissberg solar cycle, but the amplitude is three-four times higher than expected (about 5%) for GCR flux modulated by solar activity, as determined solely by the sunspot number. The cosmogenic 44Ti is a suitable radioisotope for this investigation, but its activity in meteorites is very low. We performed these measurements in the underground low level counting station of Monte dei Cappuccini in Torino, by means of a big hyperpure Ge crystal (approximately 2 kg) in selective coincidence with a heavy NaI (T1) scintillation detector (approximately 28 kg). This system allows a reliable and non destructive measurement of 44Ti (44Sc) in meteorites of 200-1200 g in weight. The background is about 1 count per day in the gamma-peak at 1157 keV of 44Sc in equilibrium with its parent 44Ti. The high stability of the performance allows long-lasting runs (approximately 10(7) s) in order to attain results with a standard deviation up to about 10%.     

New instrumentation for optical measuring of oxygen in gas or dissolved in liquids.

The optical oxygen sensor is a novel device for the determination of oxygen in gases or dissolved in liquids. It is based on the measurement principle of fluorescence quenching, which is completely different from that of polarographic oxygen sensors (today the most widespread devices of oxygen detection). The new instrument offers features and advantages, which render it not only a realistic alternative, but, for specific applications, make it superior to existing electrochemical methods. The system is based on low-cost semiconductor devices (light-emitting diodes, photodiodes, low-cost analogue and digital components) and new LED-compatible oxygen-sensitive membranes. The flow cell of the instrument may be thermostatted and the sensor can be calibrated by a simple two-point calibration procedure. The optical oxygen sensor is particularly suitable for measuring dissolved oxygen in respirometry, since no oxygen is consumed by the device and the signal is independent of sample flowrate or stirring speed. Typical fields of application are monitoring of oxygen in ground and drinking water, in process control in bioreactors and in breath gas and blood gas analysis.     

Aiming at a 1-cm Orbit for Low Earth Orbiters: Reduced-Dynamic and Kinematic Precise Orbit Determination

The computation of high-accuracy orbits is a prerequisite for the success of Low Earth Orbiter (LEO) missions such as CHAMP, GRACE and GOCE. The mission objectives of these satellites cannot be reached without computing orbits with an accuracy at the few cm level. Such a level of accuracy might be achieved with the techniques of reduced-dynamic and kinematic precise orbit determination (POD) assuming continuous Satellite-to-Satellite Tracking (SST) by the Global Positioning System (GPS). Both techniques have reached a high level of maturity and have been successfully applied to missions in the past, for example to TOPEX/POSEIDON (T/P), leading to (sub-)decimeter orbit accuracy. New LEO gravity missions are (to be) equipped with advanced GPS receivers promising to provide very high quality SST observations thereby opening the possibility for computing cm-level accuracy orbits. The computation of orbits at this accuracy level does not only require high-quality GPS receivers, but also advanced and demanding observation preprocessing and correction algorithms. Moreover, sophisticated parameter estimation schemes need to be adapted and extended to allow the computation of such orbits. Finally, reliable methods need to be employed for assessing the orbit quality and providing feedback to the different processing steps in the orbit computation process. This revised version was published online in August 2006 with corrections to the Cover Date.     

Robust Kalman filter design for predictive wind shear detection

Severe low-altitude wind shear is a threat to aviation safety. Newly developed airborne sensors measure the radial component of wind along a line directly in front of an aircraft. The authors use optimal estimation theory to define a detection algorithm to warn of hazardous wind shear from these sensors. To achieve robustness, a wind shear detection algorithm must distinguish threatening wind shear from less hazardous gustiness, despite variations in wind shear structure. Statistical analysis methods to refine wind shear detection algorithm robustness are presented. Computational methods predict the ability to warn of severe wind shear and avoid false warning. Comparative capability of the detection algorithm as a function of its design parameters is determined, identifying designs that provide robust detection of severe wind shear     

Role of BIT in support system maintenance and availability

The role of built in test (BIT) in electronic systems has grown in prominence with the advances in system complexity and concern over maintenance lifecycle costs of large systems. In an environment where standards drive system designs (and provide an avenue for focused advancement in technology), standards for BIT are very much in an evolutionary state. The reasons for advancing the effectiveness of BIT include reduced support overhead, greater, confidence in operation, and increased system availability. The cost of supporting military electronic systems (avionics, communications, and weapons systems) has driven much of the development in BIT technology. But what about the systems that support these end items that contain test and measurement instrumentation - such as automatic test equipment (ATE), simulators and avionics development suites? There has also been a beneficial effect on the maintenance and availability of these systems due to the infusion of BIT into their component assemblies. But the effect has been much more sporadic and fragmented. This paper looks at the state of BIT in test and measurement instruments, explain its affect on system readiness, and present ideas on how to improve BIT technologies and standards. This will not provide definitive answers to BIT development questions, since the factors that affect it are specific to the instrument itself. The topics covered in this paper are: definitions of built-in test, instrument BIT history, importance of BIT fault coverage and isolation in support systems, overview of BIT development process issues that limit the effectiveness of BIT Standards related to instrument BIT, making BIT more effective in support system maintenance and availability and conclusions.     

Chromosome instability of HPRT-mutant subclones induced by ionising radiation of various LET.

The induction of HPRT-mutations and survival of Chinese hamster cells (line B11ii-FAF28, clone 431) were studied after irradiation by 4He and 12C-ions of various LET (20-360 keV/micrometers), produced by the U-200 heavy ion accelerator. The RBE increases with LET up to the maximum at 100-200 keV/micrometers and then decreases. Cytogenetic analysis was performed on the HPRT-mutant subclones selected from unirradiated Chinese hamster V-79 cells and from HPRT-mutant subclones that arose after exposure to gamma-rays, 1 GeV protons and 14N-ions (LET-77 keV/micrometers), produced by the synchrophasotron and the U-400M heavy ion accelerator. Slow growing mutant subclones were observed. The cytogenetic properties of individual clones were highly heterogeneous and chromosome instability was observed in both spontaneous and radiation-induced mutants. Chromosome instability was highest among spontaneous mutants and decreased with increasing LET.