The influence of microgravity on invasive growth in Saccharomyces cerevisiae

This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies grown on semi-solid agar medium was reduced under microgravity in the Σ1278b laboratory strain but not in the CMBSESA1 industrial strain. This was supported by the Σ1278b proteome map under microgravity conditions, which revealed upregulation of proteins linked to anaerobic conditions. The Σ1278b strain showed a reduced invasive growth in the center of the yeast colony. Bud scar distribution was slightly affected, with a switch toward more random budding. Together, microgravity conditions disturb spatially programmed budding patterns and generate strain-dependent growth differences in yeast colonies on semi-solid medium.     

Crop yield and light/energy efficiency in a closed ecological system: Laboratory Biosphere experiments with wheat and sweet potato.

Two crop growth experiments in the soil-based closed ecological facility, Laboratory Biosphere, were conducted from 2003 to 2004 with candidate space life support crops. Apogee wheat (Utah State University variety) was grown, planted at two densities, 400 and 800 seeds m-2. The lighting regime for the wheat crop was 16 h of light-8 h dark at a total light intensity of around 840 micromoles m-2 s-1 and 48.4 mol m-2 d-1 over 84 days. Average biomass was 1395 g m-2, 16.0 g m-2 d-1 and average seed production was 689 g m-2 and 7.9 g m-2 d-1. The less densely planted side was more productive than the denser planting, with 1634 g m-2 and 18.8 g m-2 d-1 of biomass vs. 1156 g m-2 and 13.3 g m-2 d-1; and a seed harvest of 812.3 g m-2 and 9.3 g m-2 d-1 vs. 566.5 g m-2 and 6.5 g m-2 d-1. Harvest index was 0.49 for the wheat crop. The experiment with sweet potato used TU-82-155 a compact variety developed at Tuskegee University. Light during the sweet potato experiment, on a 18 h on/6 h dark cycle, totaled 5568 total moles of light per square meter in 126 days for the sweet potatoes, or an average of 44.2 mol m-2 d-1. Temperature regime was 28 +/- 3 degrees C day/22 +/- 4 degrees C night. Sweet potato tuber yield was 39.7 kg wet weight, or an average of 7.4 kg m-2, and 7.7 kg dry weight of tubers since dry weight was about 18.6% wet weight. Average per day production was 58.7 g m-2 d-1 wet weight and 11.3 g m-2 d-1. For the wheat, average light efficiency was 0.34 g biomass per mole, and 0.17 g seed per mole. The best area of wheat had an efficiency of light utilization of 0.51 g biomass per mole and 0.22 g seed per mole. For the sweet potato crop, light efficiency per tuber wet weight was 1.33 g mol-1 and 0.34 g dry weight of tuber per mole of light. The best area of tuber production had 1.77 g mol-1 wet weight and 0.34 g mol-1 of light dry weight. The Laboratory Biosphere experiment's light efficiency was somewhat higher than the USU field results but somewhat below greenhouse trials at comparable light levels, and the best portion of the crop at 0.22 g mol-1 was in-between those values. Sweet potato production was overall close to 50% higher than trials using hydroponic methods with TU-82-155 at NASA JSC. Compared to projected yields for the Mars on Earth life support system, these wheat yields were about 15% higher, and the sweet potato yields averaged over 80% higher.     

Massive close binaries: Observational characteristics

Theoretically predicted evolutionary phases of massive close binaries are compared with the observations. For the evolution up to the High-Mass X-ray Binary (HMXB) phase there is fair agreement between theory and observation. Beyond the HMXB phase there is much uncertainty. Notably it is puzzling why we observe so few systems consisting of a helium star and a neutron star (Cygnus X-3 is the only one found so far), and why the incidence of double neutron stars is so low. A better understanding of Common Envelope evolution is required in order to answer these questions. The role of velocity kicks imparted to neutron stars during supernova collapse is discussed. Such kicks might cause many runaway OB stars to be single.     

A European view on UNISPACE III follow-up

Preparations for the third UN Conference on the Exploration and Peaceful Uses of Outer Space (UNISPACE III) were intense. The conference itself was a success. But what forms will the follow-up take? Just reading the 150-page report is an effort in itself. Having played a central part in the preparations and organization, Europe fully appreciates the need to build on the spirit of cooperation which emerged from UNISPACE III. In November 1999, the European States gathered to analyze the results of the conference and to set a course for their future participation in the United Nations Programme on Space Applications (UNPSA), which is mainly done through ESA, and for their participation in the United Nations Committee on the Peaceful Uses of Outer Space (UNCOPUOS), which is done through coordination among ESA Member States. This article presents the authors’ personal accounts of the results of the European efforts around UNISPACE III and shows how ‘European foreign policy’ can work in international space policy. It also seeks to illustrate Europe's commitment to putting space technology to work for the benefit of development throughout the world.     

Solar maximum mission experiment: Early results of the hard X-ray imaging experiment

We have selected four widely different flares from the early period of operations of the Hard X-Ray Imaging Spectrometer (HXIS) on SMM to illustrate the characteristic imaging properties of this experiment. For the small flare of April 4, 1980, we demonstrate the instrument's capability for locating a compact source. In the weak, but extensive, flare of April 6 we show how well the instrument can display spatial structure, and also the low level of the instrument background. In the 1B flare of April 7 we are able to locate positions of the X-ray emission in the soft and hard channels, and estimate the positional variations of the emission patches. Finally, in the IN flare of April 10, which produced the strongest hard X-ray burst we have seen so far, we repeat some of the studies made for the April 7 event, and also demonstrate the capability of the HXIS instrument to study the development, with high time resolution, of individual 8″ × 8″ elements of the flare.     

LONG-TERM RIGOROUS NUMERICAL INTEGRATION OF NAVIER-STOKES EQUATION BY NEWTON-GMRES ITERATION

The recent result of an orbit continuation algorithm has provided a rigorous method for long-term numer- ical integration of an orbit on the unstable manifold of a periodic solution. This algorithm is matrix-free and em- ploys a combination of the Newton-Raphson method and the Krylov subspace method. Moreover, the algorithm adopts a multiple shooting method to address the problem of orbital instability due to long-term numerical integra- tion. The algorithm is described through computing the extension of unstable manifold of a recomputed Nagata~s lower-branch steady solution of plane Couette flow, which is an example of an exact coherent state that has recently been studied in subcritical transition to turbulence.     

Orbit determination for the GOCE satellite

Precise Orbit Determination (POD) for the Gravity field and steady-state Ocean Circulation Explorer (GOCE), the first core explorer mission by the European Space Agency (ESA), forms an integrated part of the so-called High-Level Processing Facility (HPF). Two POD chains have been set up referred to as quick-look Rapid and Precise Science Orbit determination or RSO and PSO, respectively. These chains make use of different software systems and have latencies of 1 day and 2 weeks, respectively, after tracking data availability. The RSO and PSO solutions have to meet a 3-dimensional (3D) position precision requirement of 50 cm and a few cm, respectively. The tracking data will be collected by the new Lagrange GPS receiver and the predicted characteristics of this receiver have been taken into account during the implementation phase of the two chains.     

Qualification and in-flight demonstration of a European tether deployment system on YES2

This paper highlights the design, qualification and mission performance of the tether deployer system on the second Young Engineers’ Satellite (YES2), that featured a tethered momentum transfer. The deployer is designed with a broad range of near-term tether applications in mind. The system contains the tether, including features to enhance safety and wound up in controlled manner onto a spool core, optical deployment sensors, a “barberpole” friction brake controlled by a stepper motor and a triple tether cutter system. To initiate the deployment a spring-based ejection system was developed, and to apply accurate momentum transfer a timer and release system is present on the subsatellite side. A small, 6 kg re-entry capsule was developed as subsatellite. On September 25th, 2007, YES2 deployed a 32 km tether in orbit and gathered a wealth of data. Confidence is gained from the mission results for use of the deployer in future missions.