Plant molecular biology in the space station era: utilization of KSC fixation tubes with RNAlater

Spaceflight experiments involving biological specimens face unique challenges with regard to the on orbit harvest and preservation of material for later ground-based analyses. Preserving plant material for gene expression analyses requires that the tissue be prepared and stored in a manner that maintains the integrity of RNA. The liquid preservative RNAlater (Ambion) provides an effective alternative to conventional freezing strategies, which are limited or unavailable in current spaceflight experiment scenarios. The spaceflight use of RNAlater is enabled by the Kennedy space center fixation tube (KFT), hardware designed to provide the necessary containment of fixatives during the harvest and stowage of biological samples in space. Pairing RNAlater with the KFT system provides a safe and effective strategy for preserving plant material for subsequent molecular analyses, a strategy that has proven effective in several spaceflight experiments. Possible spaceflight scenarios for the use of RNAlater and KFTs are explored and discussed.     

Initial characterization of the microgravity environment of the international space station: increments 2 through 4

The primary objective of the International Space Station (ISS) is to provide a long-term quiescent environment for the conduct of scientific research for a variety of microgravity science disciplines. This paper reports to the microgravity scientific community the results of an initial characterization of the microgravity environment on the International Space Station for increments 2 through 4. During that period almost 70,000 hours of station operations and scientific experiments were conducted. 720 hours of crew research time were logged aboard the orbiting laboratory and over half a terabyte of acceleration data were recorded and much of that was analyzed. The results discussed in this paper cover both the quasi-steady and vibratory acceleration environment of the station during its first year of scientific operation. For the quasi-steady environment, results are presented and discussed for the following: the space station attitudes Torque Equilibrium Attitude and the X-Axis Perpendicular to the Orbital Plane; station docking attitude maneuvers; Space Shuttle joint operation with the station; cabin de-pressurizations and the station water dumps. For the vibratory environment, results are presented for the following: crew exercise, docking events, and the activation/de-activation of both station life support system hardware and experiment hardware. Finally, a grand summary of all the data collected aboard the station during the 1-year period is presented showing where the overall quasi-steady and vibratory acceleration magnitude levels fall over that period of time using a 95th percentile benchmark.     

The Swift Ultra-Violet/Optical Telescope

The Ultra-Violet/Optical Telescope (UVOT) is one of three instruments flying aboard the Swift Gamma-ray Observatory. It is designed to capture the early (∼1 min) UV and optical photons from the afterglow of gamma-ray bursts in the 170–600 nm band as well as long term observations of these afterglows. This is accomplished through the use of UV and optical broadband filters and grisms. The UVOT has a modified Ritchey–Chrétien design with micro-channel plate intensified charged-coupled device detectors that record the arrival time of individual photons and provide sub-arcsecond positioning of sources. We discuss some of the science to be pursued by the UVOT and the overall design of the instrument.     

Electromagnetic Effects of Aircraft Wake-Active Feuillet Interaction

A mechanism by which an aircraft wake can interact strongly with the electromagnetic radiation present in an active elevated anomalous refractivity region (active feuillet) is analyzed. The aircraft wake structure, assumed to consist of twin contrarotating vortices plus entrained irrotational gas, trailing behind the wings of typical large aircraft is shown to be capable of descending a distance of approximately 3 wing span distances and attaining a length of the order of 10 km, prior to instability-induced disruption. The parcel of air such a descending coherent wake structure can convey into an active feuillet is demonstrated to alter significantly the local refractive index of the duct and induce substantial radiation spillage. The general characteristics of the electromagnetic radiation produced by this interaction process (i.e., scattering by diaphanous objects) is described.     

Synchronization effects on probability of pulse train interception

Expressions for the probability of coincidence between pairs of pulse trains are examined. The application to systems of interest in the field of electronic warfare is emphasized. Theoretical results for the limit of uncorrelated pulse trains are reviewed and new theory is developed to cover the case of coincidence between uniform pulse trains where the coincidence phenomena may be highly correlated. Monte Carlo experiments show that increasing the jitter level on one of the pulse trains causes a transition between the correlated and uncorrelated regimes     

Formation of replicating saponite from a gel in the presence of oxalate: implications for the formation of clay minerals in carbonaceous chondrites and the origin of life

The potential role of clay minerals in the abiotic origin of life has been the subject of ongoing debate for the past several decades. At issue are the clay minerals found in a class of meteorites known as carbonaceous chondrites. These clay minerals are the product of aqueous alteration of anhydrous mineral phases, such as olivine and orthopyroxene, that are often present in the chondrules. Moreover, there is a strong correlation in the occurrence of clay minerals and the presence of polar organic molecules. It has been shown in laboratory experiments at low temperature and ambient pressure that polar organic molecules, such as the oxalate found in meteorites, can catalyze the crystallization of clay minerals. In this study, we show that oxalate is a robust catalyst in the crystallization of saponite, an Al- and Mg-rich, trioctahedral 2:1 layer silicate, from a silicate gel at 60°C and ambient pressure. High-resolution transmission electron microscopy analysis of the saponite treated with octadecylammonium (n(C)=18) cations revealed the presence of 2:1 layer structures that have variable interlayer charge. The crystallization of these differently charged 2:1 layer silicates most likely occurred independently. The fact that 2:1 layer silicates with variable charge formed in the same gel has implications for our understanding of the origin of life, as these 2:1 clay minerals most likely replicate by a mechanism of template-catalyzed polymerization and transmit the charge distribution from layer to layer. If polar organic molecules like oxalate can catalyze the formation of clay-mineral crystals, which in turn promote clay microenvironments and provide abundant adsorption sites for other organic molecules present in solution, the interaction among these adsorbed molecules could lead to the polymerization of more complex organic molecules like RNA from nucleotides on early Earth.     

Performance of an adaptive detection algorithm; rejection ofunwanted signals

The analysis of an adaptive detection algorithm described previously by the author (1985, 1986) is extended. Previously, the performance was evaluated for the case of a signal corresponding exactly to the steering vector used in the derivation of the algorithm. Here the performance for signals arriving from other directions is evaluated. It is shown that these signals are rejected much more strongly than would be suggested by the sidelobe levels of the adapted patterns themselves     

Leisure time activities in space: a survey of astronauts and cosmonauts

Questionnaires were returned from 54 astronauts and cosmonauts which addressed preferences for media and media-generated subjects that could be used to occupy leisure time in space. Ninety-three percent of the respondents had access to records or audio cassettes, and cosmonauts had greater access than astronauts to multiple media. Cosmonauts and long-duration space travelers reported that they missed various media more than their astronaut and short-duration counterparts. Media subjects that related to international events, national events and historical topics were rated as most preferable by all respondents and by several of the respondent groups. The findings are discussed in terms of their relevance for occupying free time during future long-duration manned space missions.     

A noncoherent adaptive detection technique

An adaptive detection technique suitable for both stationary and nonstationary noise environments based upon a generalized likelihood ratio test (GLRT) formulation is presented. The detector, which is statistically equivalent to a special form of the Wilks's lambda test, noncoherently combines the information contained in a pulse train of arbitrary length for decision-making purposes. The probability density function of the test under the noise only hypothesis is shown to be central χ². Under the signal plus noise hypothesis, an exact statistical characterization of the test cannot be obtained, and, therefore, a Chernoff bound is derived. Results in terms of the probability of detection versus signal-to-noise ratio (SNR) obtained from Monte Carlo simulation, the Chernoff bound, and the optimal matched filter case are examined. The performance of the noncoherent detector is shown to be a function of the covariance matrix estimate and the number of data samples     

The Emirates Mars Mission (EMM) Emirates Mars InfraRed Spectrometer (EMIRS) Instrument

Space Science Reviews - Modern observatories have revealed the ubiquitous presence of magnetohydrodynamic waves in the solar corona. The propagating waves (in contrast to the standing waves) are...