Clinorotation reduces number, but not size, of cartilaginous nodules formed in micromass cultures of mouse limbbud cells.

In previous studies we used a ground based model to investigate the cellular responses to microgravity by exposing micromass cultures of embryonic limb cells to simulated weightlessness on a clinostat. Cultures set up in T-flasks and rotated at 30 rpm showed that clinostatted cultures had less chondrocyte differentiation than stationary or rotation controls, as assessed by number of nodules/culture stained with cartilage specific Alcian blue. In the current study, nodule size and shape of these nodules was assessed by interactive measurement of area, perimeter, circularity, and equivalent diameters, using the Optimas imaging software. Results show no significant difference in any of the measurements, indicating that clinorotation has no effect on expansion of the nodules either by differentiation of cells within the nodule, or by recruitment of cells into the nodule. The reduction in number of nodules without an alteration in size and shape indicates that the effect of simulated microgravity is to reduce the cell interactions required for the initial condensation of cells into a nodule, probably by interference with cell adhesion molecules.     

Design of the HEAO Main Bus Shunt Regulator

The High Energy Astronomy Observatory (HEAO) main bus regulator contains a redundant error amplifier (REA) and a 12-section sequenced shunt transconductance amplifier (STA) in which not more than three and not less than one shunt section are in an active control mode regardless of a failure in any one of the twelve sections. The part of the work concerned with the shaping of the frequency response of the REA and STA minor feedback loops and of the teolator major feedback loop is described. Emphasis is on the application of a general design technique embodying a "design-analyze-measure iteration loop," in which a first-cut design and analysis is corrected ard improved after experimental observation of discrepancies and deficiencies. In particular, an incipient high-frequency oscillation was thereby reliably eliminated. It is demonstrated that analysis techniques involving a minimum of algebra can provide full quantitative information on an efficient and reliable system design.     

Residual stresses and endurance strength under tension-compression in the conditions of stress concentration

Residual stresses and endurance strength of specimens with stress concentrators are considered. The results obtained make it possible to predict the fatigue limit of strengthened parts with concentrators in the case of tension-compression.