Extreme dryness and DNA-protein cross-links.

Exposure of fungal conidia (Aspergillus ochraceus) or spores of Bacillus subtilis to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered water activity results in an increasing number of cross-links between DNA and proteins. In fungal conidia these cross-links are detected after selective iodination (125 J) of the DNA-bound proteins followed by gel electrophoresis and subsequent autoradiography. Another approach is the labelling of DNA with 32P by means of nick translation and the detection of differences in the electrophoretic mobility of DNA before and after digestion with proteinase K of proteins bound to DNA.     

Reduced lymphocyte activation in space: role of cell-substratum interactions.

We investigated the effect of substratum adhesiveness on stimulated lymphocyte blastogenesis by reducing and blocking cell adhesion with poly (2-hydroxyethyl methacrylate) (poly-HEMA) in a simple on-ground system. Cells grown on medium-thick and thick poly-HEMA films were rounded in shape and displayed no signs of spreading. By contrast, on tissue culture plastic and very thin poly-HEMA films, they showed clear signs of spreading. The mitogenic response of lymphocytes grown on thick poly-HEMA films was reduced by up to 68% of the control (tissue culture plastic). Interferon-gamma production was near zero when the cells were grown on the least adhesive substratum. On uncoated plastic, activated lymphocytes subjected to high gravity (20g) exhibited an increased proliferation rate (40%) compared with 1g. By contrast, on poly-HEMA, high gravity did not improve lymphocyte responsiveness. These results show that activated lymphocytes need to anchor and spread prior to achieving an optimal proliferation response. We conclude that decreased lymphocyte adhesion could contribute to the depressed in vitro lymphocyte responsiveness found in the microgravity conditions of space flight.     

The statistical properties of coronal mass ejections during 1979–1981

The Solwind coronagraph on the P78-1 earth-orbiting satellite has been monitoring the Sun routinely at 10-minute intervals during the 5-year interval from April, 1979 to the present. In a statistical analysis of about 1000 mass ejections observed through the end of 1981, we find an average occurrence rate of 1.8 mass ejections per day. Histograms of speed, central latitude, angular span, brightness, and other parameters have been constructed, and properties such as shape classification have been tabulated. These characteristics are summarized for these years near sunspot maximum. The average speed and mass estimate are found to be similar to those found at the declining phase of the previous sunspot cycle. The angular span and central latitude distributions are quite different than seen during the declining phase, and are very dependent upon structural class. The fluctuations in the occurrence rate of CMEs does not seem to match the fluctuations in the sunspot number. There is a tendency for high speed CMEs to occur more frequently in 1981 than in 1980, and more frequently in 1980 than in 1979.     

Climate Observations - The Instrumental Record

A survey is given of the available instrumental data for monitoring and analysis of climatic variations. We focus on temperature measurements, both over land and ocean, at the surface and aloft.Over land, the older observations were subject to exposure changes which may not have been fully compensated. The effects of urbanization have been largely avoided in studies of climatic change over the last 150 years. There are few records for pre-1850 outside Europe and eastern North America, and the global network shows a recent decline. Over the ocean, sea surface temperature (SST) has been measured using buckets, engine intakes, hull sensors, buoys, and satellites. Many of these data have been effectively homogenized, but new challenges arise as observing systems evolve. Available SST and marine air temperature datasets begin in the 1850s. The data are concentrated in shipping lanes especially before 1900, and very sparse during the world wars, but additional historical data are being digitized.The radiosonde record is short (40 years) and has major gaps over the oceans, tropics and Southern Hemisphere. Instrumental heterogeneities are beginning to be assessed and removed using physical and statistical techniques. The MSU record is complete but only began in 1979, and is not highly resolved in the vertical: major biases, mainly affecting the lower-tropospheric retrieval, have been reduced as a result of recent analyses.Advanced interpolation or data-assimilation techniques are being applied to these data, but the results must be interpreted with care.     

Relationship Between Substorm Auroras and Processes in the Near-Earth Magnetotail

Although the auroral substorm has been long regarded as a manifestation of the magnetospheric substorm, a direct relation of active auroras to certain magnetospheric processes is still debatable. To investigate the relationship, we combine the data of the UV imager onboard the Polar satellite with plasma and magnetic field measurements by the Geotail spacecraft. The poleward edge of the auroral bulge, as determined from the images obtained at the LHBL passband, is found to be conjugated with the region where the oppositely directed fast plasma flows observed in the near-Earth plasma sheet during substorms are generated. We conclude that the auroras forming the bulge are due to the near-Earth reconnection process. This implies that the magnetic flux through the auroral bulge is equal to the flux dissipated in the magnetotail during the substorm. Comparison of the magnetic flux through the auroral bulge with the magnetic flux accumulated in the tail lobe during the growth phase shows that these parameters have the comparable values. This is a clear evidence of the loading–unloading scheme of substorm development. It is shown that the area of the auroral bulge developing during substorm is proportional to the total (magnetic plus plasma) pressure decrease in the magnetotail. These findings stress the importance of auroral bulge observations for monitoring of substorm intensity in terms of the magnetic flux and energy dissipation.     

Analyse du fonctionnement d'un propulseur a éjection modulée

ONERA developed, for studying the response of a propellant to a pressure or velocity fluctuation, an experimental rocket engine whose nozzle throat area can be modulated by a toothed disk.The paper presents a linearized theory of the functioning of this engine in the low frequency domain, i.e. when there is no wave propagation within the combuster.To describe the functioning of this motor, the Ryazantsev-Novozhilov method, which assumes that the gas response is instantaneous, is used. This analysis takes into account the erosion and radiation effects, the combustion efficiency and the thermal losses through the walls.Two particular cases are described, for two values of the Damköhler parameter $\text{D}{}_1\text{=}\text{t}{}_{\mathrm{c}}\text{t}{}_{\mathrm{th}}$, where t_c is the residence time in the combuster and t_th the characteristic thermal time of the heat penetration into the solid propellant. These two cases correspond, one to a classical propellant D₁ > 1, the other to a particular propellant of low burning rate ($\text{J}{}_{\mathrm{b}}\text{? 0.2}\text{to}\text{0.4}\text{mm s}{}^{\mathrm{?1}}$) D₁ < 1. The stability conditions are analysed as well as the pressure amplitute and phase as a function of the nozzle throat modulation frequency.Still in linearized theory, the complete solutions of the problem are presented, using a method of numerical resolution.