Influence of microgravity on mitogen binding and cytoskeleton in Jurkat cells.

The effects of microgravity on Jurkat cells--a T-lymphoid cell line--was studied on a sounding rocket flight. An automated pre-programmed instrument permitted the injection of fluorescent labelled concanavalin A (Con A), culture medium and/or fixative at given times. An in-flight 1 g centrifuge allowed the comparison of the data obtained in microgravity with a 1 g control having the same history related to launch and re-entry. After flight, the cells fixed either at the onset of microgravity or after a or 12 minute incubation time with fluorescent concanavalin A were labelled for vimentin and actin and analysed by fluorescence microscopy. Binding of Con A to Jurkat cells is not influenced by microgravity, whereas patching of the Con A receptors is significantly lower. A significant higher number of cells show changes in the structure of vimentin in microgravity. Most evident is the appearance of large bundles, significantly increased in the microgravity samples. No changes are found in the structure of actin and in the colocalisation of actin on the inner side of the cell membrane with the Con A receptors after binding of the mitogen.     

Gravitropism and phototropism of oat coleoptiles: post-tropic autostraightening and tissue shrinkage during tropism.

We measured changes in length on the two opposite sides of the red-light-grown oat (Avena sativa L.) coleoptiles subjected to either gravitropic or phototropic stimulation and subsequently rotated on a horizontal clinostat. The length measurement was conducted using three 5 mm-long zones delimited by ink markers from the tip. Curvature of each zone was analyzed from the length difference between the two sides. Gravitropism was induced by displacing the seedling from the vertical by 30 degrees or 90 degrees for 25 min. Phototropism was induced by exposing the coleoptile to unilateral blue light for 30 s, which provided a fluence (1.0 micromoles m-2) optimal for the pulse-induced positive phototropism or a lower, suboptimal fluence (0.03 micromoles m-2). After negatively gravitropic bending, the upper two zones straightened rapidly at either displacement angle. After positively phototropic bending, straightening occurred, but only in the top zone and at the lower fluence. The upper two zones straightened rapidly, however, when bilateral blue light (30 s; 15 micromoles m-2 from either direction) was applied 25 min after unilateral stimulation at the higher fluence. Bilateral blue light alone induced no curvature. These results confirm that the straightening of gravitropically bent coleoptiles is autonomic, and suggest that a similar autonomic response participates in the straightening of phototropically bent coleoptiles. Suppression of elongation on the concave side of the coleoptile mainly accounted for gravitropic and phototropic curvatures. The concave side of the top zone shrank during both tropisms. This shrinkage progressed at a high rate from the beginning of curvature response, suggesting that a drop in turgor pressure is the main and direct cause of the shrinkage.     

Signal transduction in T lymphocytes--a comparison of the data from space, the free fall machine and the random positioning machine.

In this paper we discuss the effect of microgravity on T cells and we present the data of studies with two new machines for 0 g simulations. Several experiments in space show that mitogenic T cell activation is lost at 0 g. Immunocytochemistry indicates that such effect is associated with changes of the cytoskeleton. Biochemical studies suggest that the lack of expression of the interleukin-2 receptor is one of the major causes of the loss of activity. In fact, interleukin-2 is the third signal required for full activation. In order to deepen our investigations we are now working with the free-fall machine, FFM, invented by D. Mesland, and with the random positioning machine, RPM, or three-dimensional clinostat, developed by T. Hoson. The FFM produces periods of free-fall lasting approximately 800 ms followed by bounces of 15-30 g lasting 45-60 ms. The RPM eliminates the effect of gravity by rotating biological specimen randomly around two orthogonal axes. While the FFM failed to reproduce the results obtained with T lymphocytes in space, the data from the RPM are in good agreement with those in real microgravity. In fact, the inhibition of the mitotic index in the RPM is 89% compared to static controls. The RPM (as the FFM) can carry markedly larger specimen than the fast rotating clinostat and thus allows to conduct comprehensive studies to select suitable biological objects for further investigations in space.     

Synergies between plant research conducted for terrestrial and for space purposes.

During the past 10 years, the main part of CELSS studies has concerned the exploration of limits of plant productivity. Very high yields were obtained in continuous and high lighting, without reaching any limit. Concepts of mineral nutrition were renewed. CELSS activities now induce a development in the techniques of image processing applied to plants in order to follow the growth, to detect stresses or diseases or to pilot harvesting robots. Notable efforts concern the development of sensors, the study of trace contaminants and the micro-organisms monitoring. In parallel, several instruments for plant culture in closed Systems were developed. The advantages of closure are emphasised in comparison with open flow systems. The concept of Artificial Ecosystems developed for space research is more and more taken into account by the scientific community. It is considered as a new tool to study basic and applied problems related to ecology and not especially concerned with space research.     

Effect of clinostating on photosynthetic apparatus of pea plants.

The photosynthetic membrane composition and low temperature fluorescence spectra were analyzed for pea chloroplasts from control and clinostated plants. Clinorotation induces a decrease in the amount of the oligomeric form of the light-harvesting chlorophyll a/b complex (LHCII) and an increase of its monomeric form. Some changes in organization of photosystem 1 (PS1) complex were revealed as well. These changes are in accordance with the variations of fluorescence characteristics and photochemical activity.     

Growth of wheat under one tenth of the atmospheric pressure.

Wheat plants were grown in twin closed growth chambers under normal and reduced atmospheric pressures. For the first 22 days from sowing, the reduced pressure was maintained at 200 hPa, and at 100 hPa for the remaining 27 days until harvest. These pressures were obtained by evacuation of the chamber and adding oxygen (170 and 79 hPa respectively) and carbon dioxide (0.65 and 1.0 hPa respectively; about 2 and 3 times above the control). Eighty-seven per cent of the final dry mass was produce under 100 hPa treatment. Growth and development of wheat are not negatively affected by low pressure treatment. Compared to the control, final dry mass increased by 76%, leaf number by 133%, and ear number by 35%, probably due to elevation of CO2. Shortening of shoot parts and increases in chlorophyll and proteins content are not in accordance with a predicted CO2 effect and could be attributed to the N2 removal and the subsequent alteration in gas diffusion rate.     

Approaches in the determination of plant nutrient uptake and distribution in space flight conditions.

The effective growth and development of vascular plants rely on the adequate availability of water and nutrients. Inefficiency in either the initial absorption, transportation, or distribution of these elements are factors which impinge on plant structure and metabolic integrity. The potential effect of space flight and microgravity conditions on the efficiency of these processes is unclear. Limitations in the available quantity of space-grown plant material and the sensitivity of routine analytical techniques have made an evaluation of these processes impractical. However, the recent introduction of new plant cultivating methodologies supporting the application of radionuclide elements and subsequent autoradiography techniques provides a highly sensitive investigative approach amenable to space flight studies. Experiments involving the use of gel based 'nutrient packs' and the radionuclides calcium-45 and iron-59 were conducted on the Shuttle mission STS-94. Uptake rates of the radionuclides between ground and flight plant material appeared comparable.     

Gravitaxis and graviperception in Euglena gracilis.

Gravitactic orientation in the flagellate Euglena gracilis is mediated by an active physiological receptor rather than a passive alignment of the cells. During a recent space flight on the American shuttle Columbia the cells were subjected to different accelerations between 0 and 1.5 x g and tracked by computerized real-time image analysis. The dependence of orientation on acceleration followed a sigmoidal curve with a threshold at < or = 0.16 x g and a saturation at about 0.32 x g. No adaptation of the cells to the conditions of weightlessness was observed over the duration of the space mission (12 days). Under terrestrial conditions graviorientation was eliminated when the cells were suspended in a medium the density of which (Ficoll) equaled that of the cell body (1.04 g/ml) and was reversed at higher densities indicating that the whole cytoplasm exerts a pressure on the respective lower membrane. There it probably activates stretch-sensitive calcium specific ion channels since gravitaxis can be affected by gadolinium which is a specific inhibitor of calcium transport in these structures. The sensory transduction chain could involve modulation of the membrane potential since ion channel blockers, ionophores and ATPase inhibitors impair graviperception.     

Mean winds of the mesosphere and lower thermosphere (60–110 km): A global distribution from radar systems (MF,Meteor, VHF)

During the last decade a large number of radars (～12) have been developed, which have produced substantial quantities of tidally-corrected mean winds data. The distribution of the radars is not global, but many areas are well covered: the Americas with Poker Flat (65°N), Saskatoon (52°N), Durham (43°N), Atlanta (34°N), Puerto Rico (18°N); Europe with Kiruna (68°), Garchy (47°N) and Monpazier (44°N); and Oceania with Christchurch (44°S), Adelaide (35°S), Townsville (20°S), and Kyoto (35°N). Zonal and meridional wind height-time cross-sections from $\text{60}\text{80}$ km (MF/Meteor Radar) to ～110 km have been prepared for the last 5–6 years. They are compared with cross-sections from CIRA-72 for zonal winds, and Groves (1969) for meridional winds.It is shown that while CIRA-72 is still a useful model for many purposes, significant differences exist between it and the new radar data. The latter demonstrate important seasonal, latitudinal, longitudinal and hemispheric variations. The new meridional cross-sections are of great value. The common features with Groves (1969) are the equatorward cells in summer near 85 km; however their strength (～10 ms^?1) and size are less. Systematic and somewhat different variations emerge at higher (?52°N) and middle (35–44°) latitudes.     

A proposed international tropical reference atmosphere up to 80 km

Motivated by the need in many aerospace applications for a meaningful reference atmosphere characteristic of the whole of the tropics in both the northern and southern hemispheres of the globe, a proposal is made here for such an atmosphere upto an altitude of 80 km. The proposal is based on balloonsonde, rocketsonde and grenade and falling sphere data, respectively, in the range of about 0–20, 20–50 and 50–80 km height. The final proposal consists of six linear segments in the temperature distribution, with temperature values in degrees Centigrade of 27, ?9, ?74, ?5, ?5, ?74 and ?74 at altitudes of 0, 9, 16, 46, 52, 75 and 80 km respectively. The sea level pressure is taken as 1010 mb, and abridged tables of quantities of interest in meteorological and aerospace applications are provided.