Detection of microlesions induced by heavy ions using liposomes filled with fluorescent dye.

In cells irradiation by heavy ions has been hypothesized to produce microlesions, regions of local damage. In cell membranes this damage is thought to manifest itself in the form of holes. The primary evidence for microlesions comes from morphological studies of cell membranes, but this evidence is still controversial, especially since holes also have been observed in membranes of normal, nonirradiated, cells. However, it is possible that damage not associated with histologically discernable disruptions may still occur. In order to resolve this issue, we developed a system for detecting microlesions based on liposomes filled with fluorescent dye. We hypothesized that if microlesions form in these liposomes as the result of irradiation, then the entrapped dye will leak out into the surrounding medium in a measurable way. Polypropylene vials containing suspensions of vesicles composed of either dipalmitoyl phosphatidylcholine, or a combination of egg phosphatidylcholine and cholesterol were irradiated at the Brookhaven National Laboratory using 56Fe ions at 1 GeV/amu. In several cases we obtained a significant loss of the entrapped dye above the background level. Our results suggest that holes may form in liposomes as the result of heavy ion irradiation, and that these holes are large enough to allow leakage of cell internal contents that are at least as large as a 1 nm diameter calcein molecule.     

Analyses of exobiological and potential resource materials in the Martian soil.

Potential Martian soil components relevant to exobiology include water, organic matter, evaporites, clays, and oxides. These materials are also resources for human expeditions to Mars. When found in particular combinations, some of these materials constitute diagnostic paleobiomarker suites, allowing insight to be gained into the probability of life originating on Mars. Critically important to exobiology is the method of data analysis and data interpretation. To that end we are investigating methods of analysis of potential biomarker and paleobiomarker compounds and resource materials in soils and rocks pertinent to Martian geology. Differential thermal analysis coupled with gas chromatography is shown to be a highly useful analytical technique for detecting this wide and complex variety of materials.     

The Electron Beam Instrument (F6) on Freja

The Electron Beam Instrument (F6) onFreja is the first attempt to apply the electron drift technique in a region of large ambient magnetic fields. The paper describes the operational principles, the technical realization, and the difficulties encountered in the derivation of the electric fields.     

Stellar infrared astronomy with EDISON

In this review the IR emission from circumstellar material is discussed, both of ionized gas and dust grains, and the astrophysical information that can be extracted from such observations. Some emphasis is placed on the possibilities of stellar IR astronomy using a large space-borne telescope, especially with respect to the much better spatial and spectral resolution of such a telescope compared to the current generation of ground-based and space IR telescopes.     

MTI data clustering and formation recognition

Image exploitation technology approaches have generally focused on the detection and spatial analysis of stationary groups of objects on the ground using various sensors. While spatial arrangement is clearly necessary in analyzing military formations, it is usually not sufficient. Typically the arrangement must be examined within some context in order to interpret a pattern of deployment. For moving objects the spatial arrangement of the group relative to the direction of motion is key to recognizing the formation. By examining ground moving target indicator (MTI) radar data over time, motion can be inferred and used to establish a context for interpreting the spatial arrangement of the data. New techniques that exploit the multitemporal nature of MTI data are described. The first is a space-time clustering technique that locates compact groups of objects that persist in time. The technique Is an application of Marr and Hildreth's edge detection methodology to the dual problem of region segmentation, or more accurately, volumetric segmentation of space-time. The second technique is based on the use of the Hough transform for recognizing moving formations such as columns, wedges, and lines abreast by analyzing the shape of clustered MTI detections (specifically the orientation of linear arrangements within the group) with respect to their direction of motion. Preliminary results from simulated MTI data sets are presented     

EUV emission from normal galaxies

Using data from the Wide Field Camera EUV all-sky survey, we have established upper limits to the EUV flux from a sample of 30 bright, nearby, non-active spiral galaxies. These galaxies were chosen to be those most likely to be detected in the EUV on the basis of (i) low interstellar absorption within our own galaxy, (ii) brightness in other wavebands, (iii) high star formation activity, and (iv) proximity. The derived EUV upper limits are restrictive, and establish for the first time that the EUV flux escaping from galaxies does not constitute a major component of their bolometric luminosity, and in particular that it cannot be the sink for the energy injected into the interstellar medium by supernova explosions, as had been suggested following the failure to detect this power in the X-ray band.     

The INFRARED SPACE OBSERVATORY (ISO)

The INFRARED SPACE OBSERVATORY (ISO), a project of the European Space Agency (ESA), will make various astronomical observations in the wavelength range of 2 to 200 μm. Two-thirds of its observing time will be available to guest observers via the traditional route of proposal submission. ISO will be launched in 1995 by an Ariane 4 from Kourou (Guyana) and be brought into a very elongated orbit with a 24hr period of which 16 hr can be used for astronomical observations. The payload module is essentially a large cryostat with a tank that will be filled with over 2000 litres of superfluid helium to keep the instruments cold (2 to 4 K) during an expected lifetime of 18 months. Inside the dewar, there is a Ritchey-Chretien telescope with a primary mirror of diameter 60cm. Four instruments, each provided by a different PI consortium, share the ISO focal plane. These instruments are : a photometer, a camera and two spectrometers — one for the wavelength range 2–45 μm and the other for 45–180 μm.     

Senrad: an advanced wideband air-surveillance radar

The generic characteristics and performance of an experimental long-range air-surveillance radar, known at the Naval Research Laboratory as Senrad, is described. Its distinguishing feature is that it can operate with simultaneous transmissions over a very wide bandwidth-from 850 to 1400 MHz. The technology and type of experimental radar equipment employed are discussed and examples are given of its performance capabilities obtained by means of very wideband operation. The unusually wide bandwidth of this radar allows 1) improved detection and tracking performance because of the absence of the nulls that are common in the antenna elevation radiation-pattern of a single-frequency radar; 2) moving target indication (MTI) without loss of targets due to blind speeds and without the need for multiple PRFs (pulse repetition frequencies); 3) accurate height finding with a fan-beam radar by taking advantage of the multipath time difference as a function of target height; 4) a form of limited target recognition based on high range-resolution; and 5) a reduction of the effectiveness of electronic countermeasures that can seriously degrade more narrowband radars     

Reconfigurable NDI controller using inertial sensor failuredetection & isolation

A modified derivation of nonlinear dynamic inversion provides the theoretical underpinnings for a reconfigurable control law for aircraft that have suffered combinations of actuator failures, missing effector surfaces, and aerodynamic changes. The approach makes use of acceleration feedback to extract information pertaining to any aerodynamic change and thus does not require a complete aerodynamic model of the aircraft. The control law does require feedback of effector positions to accommodate actuator dynamics. Both accelerometer and rate gyro failure detection and isolation (FDI) systems are implemented, allowing up to three independent failures for each FDI system as long as they are in different axes. Nonlinear simulation results show that the FDI systems improve the robustness to accelerometer/rate gyro uncertainties. An advanced tailless aircraft model is used to demonstrate the concepts. The simulation includes accelerometer and rate gyro noise and bias, failures due to accelerometers, rate gyros, and actuators, and modeled missing surfaces that cause airplane aerodynamic changes     

Paramecium--a model system for studying cellular graviperception.

Experiments under varied gravitational accelerations as well as in density-adjusted media showed that sensation of gravity in protists may be linked to the known principles of mechanosensation. Paramecium, a ciliate with clear graviresponses (gravitaxis and gravikinesis) is an ideal model system to prove this hypothesis since the ciliary activity and thus the swimming behaviour is controlled by the membrane potential. It has also been assumed that the cytoplasmic mass causes a distinct stimulation of the bipolarly distributed mechano-sensitive K+ and Ca2+ ion channels in the plasma membrane in dependence of the spatial orientation of the cell. In order to prove this hypothesis, different channel blockers are currently under investigation. Gadolinium did not inhibit gravitaxis in Paramecium, showing that it does not specifically block gravireceptors. Further studies concentrated on the question of whether second messengers are involved in the gravity signal transduction chain. Exposure to 5 g for up to 10 min led to a significant increase in cAMP.