Extreme dryness and DNA-protein cross-links.

Exposure of fungal conidia (Aspergillus ochraceus) or spores of Bacillus subtilis to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered water activity results in an increasing number of cross-links between DNA and proteins. In fungal conidia these cross-links are detected after selective iodination (125 J) of the DNA-bound proteins followed by gel electrophoresis and subsequent autoradiography. Another approach is the labelling of DNA with 32P by means of nick translation and the detection of differences in the electrophoretic mobility of DNA before and after digestion with proteinase K of proteins bound to DNA.     

Reduced lymphocyte activation in space: role of cell-substratum interactions.

We investigated the effect of substratum adhesiveness on stimulated lymphocyte blastogenesis by reducing and blocking cell adhesion with poly (2-hydroxyethyl methacrylate) (poly-HEMA) in a simple on-ground system. Cells grown on medium-thick and thick poly-HEMA films were rounded in shape and displayed no signs of spreading. By contrast, on tissue culture plastic and very thin poly-HEMA films, they showed clear signs of spreading. The mitogenic response of lymphocytes grown on thick poly-HEMA films was reduced by up to 68% of the control (tissue culture plastic). Interferon-gamma production was near zero when the cells were grown on the least adhesive substratum. On uncoated plastic, activated lymphocytes subjected to high gravity (20g) exhibited an increased proliferation rate (40%) compared with 1g. By contrast, on poly-HEMA, high gravity did not improve lymphocyte responsiveness. These results show that activated lymphocytes need to anchor and spread prior to achieving an optimal proliferation response. We conclude that decreased lymphocyte adhesion could contribute to the depressed in vitro lymphocyte responsiveness found in the microgravity conditions of space flight.     

The statistical properties of coronal mass ejections during 1979–1981

The Solwind coronagraph on the P78-1 earth-orbiting satellite has been monitoring the Sun routinely at 10-minute intervals during the 5-year interval from April, 1979 to the present. In a statistical analysis of about 1000 mass ejections observed through the end of 1981, we find an average occurrence rate of 1.8 mass ejections per day. Histograms of speed, central latitude, angular span, brightness, and other parameters have been constructed, and properties such as shape classification have been tabulated. These characteristics are summarized for these years near sunspot maximum. The average speed and mass estimate are found to be similar to those found at the declining phase of the previous sunspot cycle. The angular span and central latitude distributions are quite different than seen during the declining phase, and are very dependent upon structural class. The fluctuations in the occurrence rate of CMEs does not seem to match the fluctuations in the sunspot number. There is a tendency for high speed CMEs to occur more frequently in 1981 than in 1980, and more frequently in 1980 than in 1979.     

Cellular parameters for track structure modeling of radiation hazard in space.

Based on irradiation with 45 MeV/u N and B ions and with Co-60 gamma rays, cellular parameters of Katz's track structure model have been fitted for the survival of V79-379A Chinese hamster lung fibroblasts. Cellular parameters representing neoplastic transformations in C3H10T/1/2 cells after their irradiation with heavy ion beams, taken from earlier work, were also used to model the radiation hazard in deep space, following the system for evaluating, summing and reporting occupational exposures proposed in 1967 by a subcommittee of NCRP. We have performed model calculations of the number of transformations in surviving cells, after a given fluence of heavy charged particles of initial energy 500 MeV/u, penetrating thick layers of cells. We take the product of cell transformation and survival probabilities, calculated along the path lengths of charged particles using cellular survival and transformation parameters, to represent a quantity proportional to the "radiation risk factor" discussed in the NCRP document. The "synergistic" effect of simultaneous charged particle transfers is accounted for by the "track overlap" mode inherent in the model of Katz.     

Recent NASA research accomplishments aboard the ISS

The activation of the US Laboratory Module "Destiny" on the International Space Station (ISS) in February 2001 launched a new era in microgravity research. Destiny provides the environment to conduct long-term microgravity research utilizing human intervention to assess, report, and modify experiments real time. As the only available pressurized space platform, ISS maximizes today's scientific resources and substantially increases the opportunity to obtain much longed-for answers on the effects of microgravity and long-term exposure to space. In addition, it evokes unexpected questions and results while experiments are still being conducted, affording time for changes and further investigation. While building and outfitting the ISS is the main priority during the current ISS assembly phase, seven different space station crews have already spent more than 2000 crew hours on approximately 80 scientific investigations, technology development activities, and educational demonstrations.     

TEC differences for the mid-latitude ionosphere in both sides of the longitudes with zero declination

Based on measurements of ground-based GPS station network, differences of the mid-latitude ionospheric TEC in the east and west sides of North America, South America and Oceania have been analyzed in this paper. Results show that for nearly all seasons from 2001 to 2010 and in both sides of the longitudes with zero declination, there exist systematic differences for the mid-latitude ionospheric TEC in the regions mentioned above and the features of these differences markedly depend upon the local time but less depend upon seasons and the level of solar activity. Theory analysis shows that the longitude variations of both declination and zonal thermospheric winds are one of important factors to cause differences of the mid-latitude ionospheric TEC in both sides of the longitudes with zero declination.     

Neural and fuzzy reconstructors for the virtual flight datarecorder

The results are presented of a comparative study evaluating the performance of neural network (NN) and fuzzy logic reconstructors (FLRs) for the development of a virtual flight data recorder (VFDK). Typical flight data recorders (FDRS) on commercial airliners do not record the aircraft control surface deflections. These dynamic parameters are critical in the investigation of an accident or an uncommanded maneuver. The results are shown relative to a VFDR based on a neural network simulator (NNS) along with a neural network reconstructor (NNR) or a FLR The NNS is trained off-line, using available flight data for the particular aircraft, for the purpose of simulating any desired dynamic output recorded in current FDRs. The NNS is then interfaced with the NNR or with the FLR. The output of the two reconstructors are the control surface deflections which minimize a performance index based on the differences between the available data from the FDR and the output from the NNS. The study tested with night data from a B737-300 shows that both schemes, the one with the NNR and the one with the FLR, provide accurate reconstructions of the control surface deflections time histories     

Structures for radar detection in compound Gaussian clutter

The problem of coherent radar target detection in a background of non-Gaussian clutter modeled by a compound Gaussian distribution is studied here. We show how the likelihood ratio may be recast into an estimator-correlator form that shows that an essential feature of the optimal detector is to compute an optimum estimate of the reciprocal of the unknown random local power level. We then proceed to show that the optimal detector may be recast into yet another form, namely a matched filter compared with a data-dependent threshold. With these reformulations of the optimal detector, the problem of obtaining suboptimal detectors may be systematically studied by either approximating the likelihood ratio directly, utilizing a suboptimal estimate in the estimator-correlator structure or utilizing a suboptimal function to model the data-dependent threshold in the matched filter interpretation. Each of these approaches is studied to obtain suboptimal detectors. The results indicate that for processing small numbers of pulses, a suboptimal detector that utilizes information about the nature of the non-Gaussian clutter can be implemented to obtain quasi-optimal performance. As the number of pulses to be processed increases, a suboptimal detector that does not require information about the specific nature of the non-Gaussian clutter may be implemented to obtain quasi-optimal performance     

Auxin polar transport in Arabidopsis under simulated microgravity conditions — Relevance to growth and development

Activity of auxin polar transport in inflorescence axes of Arabidopsis thaliana grown under simulated microgravity conditions was studied in relation to the growth and development. Seeds were germinated and allowed to grow on an agar medium in test tubes on a horizontal clinostat. Horizontal clinostat rotation substantially reduced the growth of inflorescence axes and the productivity of seeds of Arabidopsis thaliana (ecotypes Landsberg erecta and Columbia), although it little affected seed germination, development of rosette leaves and flowering. The activity of auxin polar transport in inflorescence axes decreased when Arabidopsis plants were grown on a horizontal clinostat from germination stage, being ca. 60% of 1 g control. On the other hand, the auxin polar transport in inflorescence axes of Arabidopsis grown in 1 g conditions was not affected when the segments were exposed to various gravistimuli, including 3-dimensional clinorotation, during transport experiments. Pin-formed mutant of Arabidopsis, having a unique structure of the inflorescence axis with no flower and extremely low levels of the activity of auxin polar transport in inflorescence axes and endogenous auxin, did not continue its vegetative growth under clinostat rotation. These facts suggest that the development of the system of auxin polar transport in Arabidopsis is affected by microgravity, resulting in the inhibition of growth and development, especially during reproductive growth.