Biodosimetry of heavyions by interphase chromosome painting

We report measurements of chromosomal aberrations in peripheral blood lymphocytes from cancer patients undergoingradiotherapy treatment. Patients with cervix or esophageal cancer were treated with 10 MV X-rays produced at a LINAC accelerator, or high-energy carbon ions produced at the HIMAC accelerator at the National Institute for Radiological Sciences (NIRS) in Chiba. Blood samples were obtained before, during, and after the radiation treatment. Chromosomes were prematurely condensed by incubation in calyculin A. Aberrations in chromosomes 2 and 4 were scored after fluorescence in situ hybridization with whole-chromosome probes. Pre-treatment samples were exposed in vitro to X-rays, individual dose-response curves for the induction of chromosomal aberrations were determined, and used as calibration curves to calculate the effective whole-body dose absorbed during the treatment. This calculated dose, based on the calibration curve relative to the induction of reciprocal exchanges, has a sharp increase after the first few fractions of the treatment, then saturates at high doses. Although carbon ions are 2–3 times more effective than X-rays in tumor sterilization, the effective dose was similar to that of X-ray treatment. However, the frequency of complex-type chromosomal exchanges was much higher for patients treated with carbon ions than X-ray.     

Solar flare forecasting model supported with artificial neural network techniques

Nowadays operational models for solar activity forecasting are still based on the statistical relationship between solar activity and solar magnetic field evolution. In order to set up this relationship, many parameters have been proposed to be the measures. Conventional measures are based on the sunspot group classification which provides limited information from sunspots. For this reason, new measures based on solar magnetic field observations are proposed and a solar flare forecasting model supported with an artificial neural network is introduced. This model is equivalent to a person with a long period of solar flare forecasting experience.     

Observations in the South Atlantic geomagnetic anomaly with intercosmos-Bulgaria-1300 during a geomagnetic storm

The region of South Atlantic Geomagnetic Anomaly (SAGA) was investigated by the Intercosmos-Bulgaria-1300 satellite, launched on August 7, 1981. On the basis of data obtained from 15 orbits during increased geomagnetic activity in August 1981, a map of the Anomaly was elaborated. Two centres of activity were identified. By means of the EMO-5 electrophotometer on board the Intercosmos-Bulgaria-1300 satellite, the atmosphere glow in lines λ 5577 Å, λ 6300 Å and λ 4278 Å was studied.     

The biological clock of Neurospora in a microgravity environment.

The circadian rhythm of conidiation in Neurospora crassa is thought to be an endogenously derived circadian oscillation; however, several investigators have suggested that circadian rhythms may, instead, be driven by some geophysical time cue(s). An experiment was conducted on space shuttle flight STS-9 in order to test this hypothesis; during the first 7-8 cycles in space, there were several minor alterations observed in the conidiation rhythm, including an increase in the period of the oscillation, an increase in the variability of the growth rate and a diminished rhythm amplitude, which eventually damped out in 25% of the flight tubes. On day seven of flight, the tubes were exposed to light while their growth fronts were marked. Some aspect of the marking process reinstated a robust rhythm in all the tubes which continued throughout the remainder of the flight. These results from the last 86 hours of flight demonstrated that the rhythm can persist in space. Since the aberrant rhythmicity occurred prior to the marking procedure, but not after, it was hypothesized that the damping on STS-9 may have resulted from the hypergravity pulse of launch. To test this hypothesis, we conducted investigations into the effects of altered gravitational forces on conidiation. Exposure to hypergravity (via centrifugation), simulated microgravity (via the use of a clinostat) and altered orientations (via alterations in the vector of a 1 g force) were used to examine the effects of gravity upon the circadian rhythm of conidiation.     

Biological nitrogen fixation under primordial Martian partial pressures of dinitrogen.

Early Earth and early Mars were similar enough such that past geochemical and climatic conditions on Mars may have also been favorable for the origin of life. However, one of the most striking differences between the two planets was the low partial pressure of dinitrogen (pN2) on early Mars (18 mb). On Earth, nitrogen is a key biological element and in many ecosystems the low availability of fixed nitrogen compounds is the main factor limiting growth. Biological fixation of dinitrogen on Earth is a crucial source of fixed nitrogen. Could the low availability of dinitrogen in the primordial Martian atmosphere have prevented the existence, or evolution of Martian microbiota? Azotobacter vinelandii and Azomonas agilis were grown in nitrogen free synthetic medium under various partial pressures of dinitrogen ranging from 780-0 mb (total atmosphere=1 bar). Below 400 mb the biomass, cell number, and growth rate decreased with decreasing pN2. Both microorganisms were capable of growth at a pN2 as low as 5 mb, but no growth was observed at a pN2 < or = 1 mb. The data appear to indicate that biological nitrogen fixation could have occurred on primordial Mars (pN2=18 mb) making it possible for a biotic system to have played a role in the Martian nitrogen cycle. It is possible that nitrogen may have played a key role in the early evolution of life on Mars, and that later a lack of available nitrogen on that planet (currently, pN2=0.2 mb) may have been involved in its subsequent extinction.     

Temperature comparison between CHAMP radio occultation and TIMED/SABER measurements in the lower stratosphere

Global Positioning System (GPS) receiver on the CHAllenging Mini-satellite Payload (CHAMP) and the Sounding of the Atmosphere using Broadband Emission Radiometry (SABER) instrument, one of four on board the TIMED satellite, provide middle atmosphere temperature profiles by Radio Occultation (RO) and limb viewing infrared emission measurements, respectively. These temperature profiles retrieved by two different techniques in the stratosphere are compared with each other using more than 1300 correlative profiles in March, September and December 2005. The over-all mean differences averaged over 15 and 35 km are approximately −2 K and standard deviation is less than 3 K. Below 20 km of altitude, relatively small mean temperature differences ∼1 K are observed in wide latitudinal range except for June (during the SABER nighttime observation). In the middle to low latitudes, between 30°S and 30°N, the temperature difference increases with height from ∼0–1 K at 15 km, to ∼−4 K at 35 km of altitude. Large temperature differences about −4 to −6 K are observed between 60°S and 30°N and 31–35 km of altitude for all months and between 0° and 30°N below 16 km during June (nighttime).     

Variations of the solar acoustic high-degree mode frequencies over solar cycle 23

Using full-disk observations obtained with the Michelson Doppler Imager (MDI) on board the Solar and Heliospheric Observatory (SOHO) spacecraft, we present variations of the solar acoustic mode frequencies caused by the solar activity cycle. High-degree (100 < ? < 900) solar acoustic modes were analyzed using global helioseismology analysis techniques over most of solar cycle 23. We followed the methodology described in details in [Korzennik, S.G., Rabello-Soares, M.C., Schou, J. On the determination of Michelson Doppler Imager high-degree mode frequencies. ApJ 602, 481–515, 2004] to infer unbiased estimates of high-degree mode parameters ([see also Rabello-Soares, M.C., Korzennik, S.G., Schou, J. High-degree mode frequencies: changes with solar cycle. ESA SP-624, 2006]). We have removed most of the known instrumental and observational effects that affect specifically high-degree modes. We show that the high-degree changes are in good agreement with the medium-degree results, except for years when the instrument was highly defocused. We analyzed and discuss the effect of defocusing on high-degree estimation. Our results for high-degree modes confirm that the frequency shift scaled by the relative mode inertia is a function of frequency and it is independent of degree.     

Pitch angle diffusion as seen by CRRES

Lifetimes and pitch angle distributions of radiation belt electrons injected into the slot region are determined from CRRES/MEA particle flux measurements. The dominant loss mechanism is presumed to be pitch angle scattering due to whistler waves, for which a theoretical formulation is available. The empirical lifetimes are compared to those from recent calculations, which rely on model whistler wave parameters. CRRES measurements of cyclotron-frequency electric fields are also presented and compared to the wave model.     

Tidal associated temperature disturbances observed at the middle atmosphere (30–65 km) by a Rayleigh lidar at 23°S

A lidar has been operated in São José dos Campos, Brazil (23.2°S, 45.8°W) since 1972, mainly dedicated to the study of mesospheric sodium at the 589 nm resonant line. The molecular Rayleigh scattering can also be used provided we limit the height to ∼75 km where the sodium scattering begins. Nevertheless, the weak signal obtained only permits the determination of density and temperature profiles by accumulating a large number of shots giving only nocturnal average profiles. Temporal variations in density and temperature on the scale of hours can however, be obtained by performing a superposed epoch analysis for a given time interval and covering a period of several days. In this way we obtained hourly mean profiles grouped by months, seasons and overall, with data acquired from 1993 to 2004. The difference between the hourly temperatures and the nocturnal means shows for some months, with enough data coverage, downward propagating structures that apparently have tidal origin. The seasonal averages show a recurrent feature with high temperatures before and low temperatures after midnight above 50 km. Some similarity is found with the GSWM model, but the observed temperature amplitudes are twice of that for the model.