Influence of microgravity on mitogen binding and cytoskeleton in Jurkat cells.

The effects of microgravity on Jurkat cells--a T-lymphoid cell line--was studied on a sounding rocket flight. An automated pre-programmed instrument permitted the injection of fluorescent labelled concanavalin A (Con A), culture medium and/or fixative at given times. An in-flight 1 g centrifuge allowed the comparison of the data obtained in microgravity with a 1 g control having the same history related to launch and re-entry. After flight, the cells fixed either at the onset of microgravity or after a or 12 minute incubation time with fluorescent concanavalin A were labelled for vimentin and actin and analysed by fluorescence microscopy. Binding of Con A to Jurkat cells is not influenced by microgravity, whereas patching of the Con A receptors is significantly lower. A significant higher number of cells show changes in the structure of vimentin in microgravity. Most evident is the appearance of large bundles, significantly increased in the microgravity samples. No changes are found in the structure of actin and in the colocalisation of actin on the inner side of the cell membrane with the Con A receptors after binding of the mitogen.     

Rocket Exhaust Scattering Effects on Incident Microwave Propagation Characteristics

This paper considers the effect of the rocket exhaust turbulence and scattering within the surrounding medium upon the propagation characteristics of incident electromagnetic waves. The exhaust is represented by a cylindrical plasma beam, diffusing through the surrounding medium. The equations of propagation of EM waves are derived for both TE and TM modes. By using a quasi-linear perturbation technique the exhaust is further separated into an inner homogeneous cylindrical plasma beam, and an outer conical inhomogeneous turbulent region. The isotropic change in the temperature of the outer region and its effects on the fluctuations in the density of electrons, collision frequency, and plasma index of refraction are analyzed in detail. It is found that the exhaust turbulence and scattering effects produce linear fluctuations in the E and H fields computed from the exhaust inner region effect. The equations of this paper can be used in the prediction of the radar cross sections and the attenuation of microwaves by rocket exhaust plumes.     

Reducing Undesirable Effects in the Human Centrifuge

The human centrifuge is used to train pilots and astronauts for flights where they are subject to large accelerations. This paper deals with the problems of simulating such linear accelerations with a rotating device and presents suitable means of reducing undesirable simulation error. The advantages of using three gimbals rather than the usual two are considered. Numerical results illustrate the problems of simulation and the improvement achieved by the use of a third gimbal.     

Fast converging adaptive processor or a structured covariance matrix

The use of adaptive linear techniques to solve signal processing problems is needed particularly when the interference environment external to the signal processor (such as for a radar or communication system) is not known a priori. Due to this lack of knowledge of an external environment, adaptive techniques require a certain amount of data to cancel the external interference. The number of statistically independent samples per input sensor required so that the performance of the adaptive processor is close (nominally within 3 dB) to the optimum is called the convergence measure of effectiveness (MOE) of the processor. The minimization of the convergence MOE is important since in many environments the external interference changes rapidly with time. Although there are heuristic techniques in the literature that provide fast convergence for particular problems, there is currently not a general solution for arbitrary interference that is derived via classical theory. A maximum likelihood (ML) solution (under the assumption that the input interference is Gaussian) is derived here for a structured covariance matrix that has the form of the identity matrix plus an unknown positive semi-definite Hermitian (PSDH) matrix. This covariance matrix form is often valid in realistic interference scenarios for radar and communication systems. Using this ML estimate, simulation results are given that show that the convergence is much faster than the often-used sample matrix inversion method. In addition, the ML solution for a structured covariance matrix that has the aforementioned form where the scale factor on the identity matrix is arbitrarily lower-bounded, is derived. Finally, an efficient implementation is presented.     

Gravitropism and phototropism of oat coleoptiles: post-tropic autostraightening and tissue shrinkage during tropism.

We measured changes in length on the two opposite sides of the red-light-grown oat (Avena sativa L.) coleoptiles subjected to either gravitropic or phototropic stimulation and subsequently rotated on a horizontal clinostat. The length measurement was conducted using three 5 mm-long zones delimited by ink markers from the tip. Curvature of each zone was analyzed from the length difference between the two sides. Gravitropism was induced by displacing the seedling from the vertical by 30 degrees or 90 degrees for 25 min. Phototropism was induced by exposing the coleoptile to unilateral blue light for 30 s, which provided a fluence (1.0 micromoles m-2) optimal for the pulse-induced positive phototropism or a lower, suboptimal fluence (0.03 micromoles m-2). After negatively gravitropic bending, the upper two zones straightened rapidly at either displacement angle. After positively phototropic bending, straightening occurred, but only in the top zone and at the lower fluence. The upper two zones straightened rapidly, however, when bilateral blue light (30 s; 15 micromoles m-2 from either direction) was applied 25 min after unilateral stimulation at the higher fluence. Bilateral blue light alone induced no curvature. These results confirm that the straightening of gravitropically bent coleoptiles is autonomic, and suggest that a similar autonomic response participates in the straightening of phototropically bent coleoptiles. Suppression of elongation on the concave side of the coleoptile mainly accounted for gravitropic and phototropic curvatures. The concave side of the top zone shrank during both tropisms. This shrinkage progressed at a high rate from the beginning of curvature response, suggesting that a drop in turgor pressure is the main and direct cause of the shrinkage.     

Signal transduction in T lymphocytes--a comparison of the data from space, the free fall machine and the random positioning machine.

In this paper we discuss the effect of microgravity on T cells and we present the data of studies with two new machines for 0 g simulations. Several experiments in space show that mitogenic T cell activation is lost at 0 g. Immunocytochemistry indicates that such effect is associated with changes of the cytoskeleton. Biochemical studies suggest that the lack of expression of the interleukin-2 receptor is one of the major causes of the loss of activity. In fact, interleukin-2 is the third signal required for full activation. In order to deepen our investigations we are now working with the free-fall machine, FFM, invented by D. Mesland, and with the random positioning machine, RPM, or three-dimensional clinostat, developed by T. Hoson. The FFM produces periods of free-fall lasting approximately 800 ms followed by bounces of 15-30 g lasting 45-60 ms. The RPM eliminates the effect of gravity by rotating biological specimen randomly around two orthogonal axes. While the FFM failed to reproduce the results obtained with T lymphocytes in space, the data from the RPM are in good agreement with those in real microgravity. In fact, the inhibition of the mitotic index in the RPM is 89% compared to static controls. The RPM (as the FFM) can carry markedly larger specimen than the fast rotating clinostat and thus allows to conduct comprehensive studies to select suitable biological objects for further investigations in space.     

Sensor alignment with Earth-centered Earth-fixed (ECEF) coordinatesystem

In this work, we formulate the multiple sensor alignment problem in an Earth-centered Earth-fixed (ECEF) coordinate system. The alignment algorithm maps the sensor measurements to the ECEF coordinates using a geodetic transformation, and attributes the discrepancies in the Earth-referenced system reported by each sensor to the sensor biases. Sensor biases are then estimated using the least squares (LS) technique. Simulated and real-life radar data are used to evaluate the performance of the proposed algorithm. Comparisons are made to those algorithms based on the standard stereographic projection     

History of Time-Frequency Technology

Present-day collision avoidance systems (CAS) of the time-frequency variety employ modes of operation similar to those of airborne equipment which has been operating in military aircraft since 1959. A fleet operational evaluation of these systems began in 1961 in aircraft of U. S. Navy Helicopter Squadron HS-4, based on the aircraft carrier U.S.S. Yorktown. This equipment utilized a local clock in each aircraft, a separate time slot for each aircraft's interrogation signal, air-to-air coarse synchronization of all stations, and sufficient free-drift stability for time slot keeping. Operational use of one-way ranging with elimination of propagation delay offsets, higher clock stability for open-loop time keeping in the SNS-64 ... AN/APN-169 family of systems began in 1964 in U. S. Air Force C-130E turboprop aircraft. In 1965, use of the EROS I collision avoidance system began during flight testing of F-4 Phantom supersonic aircraft.     

One-to-one relationship between low latitude whistlers and conjugate source lightning discharges and their propagation characteristics

One-to-one relation with its causative lightning discharges and propagation features of night-time whistlers recorded at low-latitude station, Allahabad (geomag. lat. 16.05°N, L = 1.08), India, from continuous observations made during 1–7 April, 2011 have been studied. The whistler observations were made using the Automatic Whistler Detector (AWD) system and AWESOME VLF receiver. The causative lightning strikes of whistlers were checked in data provided by World-Wide Lightning Location Network (WWLLN). A total of 32 whistlers were observed out of which 23 were correlated with their causative lightnings in and around the conjugate location (geom. lat. 9.87°S) of Allahabad. A multi-flash whistler is also observed on 1 April with dispersions 15.3, 17.5 and 13.6 s^1/2. About 70% (23 out of 32) whistlers were correlated with the WWLLN detected causative lightnings in the conjugate region which supports the ducted mode of propagation at low latitude. The multi-flash and short whistlers also propagated most likely in the ducted mode to this station.