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511.
Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells. 总被引:7,自引:0,他引:7
The cytoskeleton is a complex network of fibers that is sensitive to environmental factors including microgravity and altered gravitational forces. Cellular functions such as transport of cell organelles depend on cytoskeletal integrity; regulation of cytoskeletal activity plays a role in cell maintenance, cell division, and apoptosis. Here we report cytoskeletal and mitochondria alterations in cultured human lymphocyte (Jurkat) cells after exposure to spaceflight and in insect cells of Drosophila melanogaster (Schneider S-1) after exposure to conditions created by clinostat rotation. Jurkat cells were flown on the space shuttle in Biorack cassettes while Schneider S-1 cells were exposed to altered gravity forces as produced by clinostat rotation. The effects of both treatments were similar in the different cell types. Fifty percent of cells displayed effects on the microtubule network in both cell lines. Under these experimental conditions mitochondria clustering and morphological alterations of mitochondrial cristae was observed to various degrees after 4 and 48 hours of culture. Jurkat cells underwent cell divisions during exposure to spaceflight but a large number of apoptotic cells was also observed. Similar results were obtained in Schneider S-1 cells cultured under clinostat rotation. Both cell lines displayed mitochondria abnormalities and mitochondria clustering toward one side of the cells which is interpreted to be the result of microtubule disruption and failure of mitochondria transport along microtubules. The number of mitochondria was increased in cells exposed to altered gravity while cristae morphology was severely affected indicating altered mitochondria function. These results show that spaceflight as well as altered gravity produced by clinostat rotation affects microtubule and mitochondria organization and results in increases in apoptosis. Grant numbers: NAG 10-0224, NAG2-985. 相似文献
512.
Y. Lin S.F. Martin O. Engvold L.H.M. Rouppe van der Voort M. van Noort 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2008
High resolution Hα images and magnetograms (0.2 arc s) of an active region were obtained in alternating time series at 42 s cadences using the Swedish 1-m Solar Telescope on 2004 August 21. The Hα filtergrams reveal an active region filament and surges consisting of thread-like structures which have widths similar to the widths of chromospheric fibrils, both recorded down to the resolution limit in the best images. All observed structures in the active region appear highly dynamic. Fibrils show counterstreaming strongly resembling the counterstreaming threads in filaments. 相似文献
513.
The specifications are presented for an imperfect automatic test system (ATS) (test frequency distribution, reliability, false alarm rate, nondetection rate) in order to account for the availability, readiness, mean time between unscheduled repairs (MTBUR), reliability, and maintenance of the system subject to monitoring and test. A time-dependent Markov model is presented, and applied in three cases, with examples of numerical results provided for preventive maintenance decisions, design of an automatic test system, buffer testing in computers, and data communications. 相似文献
514.
J F Bottollier-Depois Q Chau P Bouisset G Kerlau L Plawinski L Lebaron-Jacobs 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,32(1):59-66
The assessment of exposure to cosmic radiation on board aircraft is one of the preoccupations of organizations responsible for radiation protection. The cosmic radiation particle flux increases with altitude and latitude and depends on the solar activity. The radiation exposure has been estimated on several airlines using transatlantic, Siberian and transequatorial routes on board subsonic and supersonic aircraft, to illustrate the effect of these parameters. Measurements have been obtained with a tissue equivalent proportional counter using the microdosimetric technique. Data have been collected at maximum solar activity in 1991-92 and at minimum in 1996-98. The lowest mean dose rate measured was 3 microSv/h during a Paris-Buenos Aires flight in 1991; the highest was 6.6 microSv/h during a Paris-Tokyo flight using a Siberian route and 9.7 microSv/h on Concorde in 1996-97. The mean quality factor is around 1.8. The corresponding annual effective dose, based on 700 hours of flight for subsonic aircraft and 300 hours for Concorde, can be estimated between 2 mSv for least-exposed routes and 5 mSv for more exposed routes. 相似文献
515.
B Biermann L J Feldman 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1994,14(8):331-340
This research aims to characterize regulation of the principal cytosolic protein kinases in maize, cultivar 'Merit' root tips, since much evidence indicates that stimuli which modulate the gravitropic response in this system act through regulation of activity of these enzymes. To this end, we have cloned a maize protein kinase belonging to a group of plant protein kinases with a catalytic domain similar in primary structure to the second messenger-regulated protein kinases known in animal and fungal systems. However, both the unique structural features conserved among plant protein kinases in this group, and lack of evidence for cyclic nucleotide signalling in plants point to operation of a novel protein kinase regulatory mechanism in plants. In order to test effects of possible regulators on protein kinase activity, we developed a sensitive method for detecting regulation of autophosphoryl labelling of protein kinases in unfractionated maize protein extracts. Regulation of protein kinase autophosphorylation in these extracts was different from that known in animals and fungi, further suggesting operation of unique protein kinase regulatory mechanisms in plants. Previous research has shown that light, or factors modulated by light, regulate plant protein kinase activity. We found that protein kinase activity was co-immunoprecipitated with the plant photoreceptor phytochrome, and was associated with phytochrome by high-affinity chemical interactions. Far-red reversibility of red-light regulation of phytochrome phosphorylation by the associated protein kinase indicates that it may modulate or transduce the light signals which lead to gravitropic sensitivity in 'Merit' maize. 相似文献
516.
J W Wilson L W Townsend J L Shinn F A Cucinotta R C Costen F F Badavi S L Lamkin 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1994,14(10):841-852
The development of the theory of high charge and energy (HZE) ion transport is reviewed. The basic solution behavior and approximation techniques will be described. An overview of the HZE transport codes currently available at the Langley Research Center will be given. The near term goal of the Langley program is to produce a complete set of one-dimensional transport codes. The ultimate goal is to produce a set of complete three-dimensional codes which have been validated in the laboratory and can be applied in the engineering design environment. Recent progress toward completing these goals is discussed. 相似文献
517.
T G Guzik S Albergo C X Chen S Costa H J Crawford J Engelage P Ferrando I Flores L Greiner F C Jones C N Knott S Ko P J Lindstrom J Mazotta J W Mitchell J Romanski R Potenza A Soutoul O Testard C E Tull C Tuve C J Waddington W R Webber J P Wefel X Zhang 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1994,14(10):825-830
The Transport Collaboration, consisting of researchers from institutions in France, Germany, Italy and the USA, has established a program to make new measurements of nuclear interaction cross sections for heavy projectiles (Z > or = 2) in targets of liquid H2, He and heavier materials. Such cross sections directly affect calculations of galactic and solar cosmic ray transport through matter and are needed for accurate radiation hazard assessment. To date, the collaboration has obtained data using the LBL Bevalac HISS facility with 20 projectiles from 4He to 58Ni in the energy range 393-910 MeV/nucleon. Preliminary results from the analysis of these data are presented here and compared to other measurements and to cross section prediction formulae. 相似文献
518.
Suboptimal joint probabilistic data association 总被引:5,自引:0,他引:5
A significant problem in multiple target tracking is the hit-to-track data association. A hit is a received signal from a target or background clutter which provides positional information. If an incorrect hit is associated with a track, that track could diverge and terminate. Prior methods for this data association problem include various optimal and suboptimal two-dimensional assignment algorithms which make hit-to-track associations. Another method is to assign a weight for the reasonable hits and use a weighted centroid of those hits to update the track. The method of weighting the hits is known as joint probabilistic data association (JPDA). The authors review the JPDA approach and a simple ad hoc approximation and then introduce a new suboptimal JPDA algorithm. Examples which compare an optimal two-dimensional assignment algorithm with the ad hoc and the new suboptimal JPDA formulation are given 相似文献
519.
The fault-tolerant air data/inertial reference unit (ADIRU) described is a key part of a fault-tolerant air data/inertial reference system (ADIRS) designed to be the inertial and air data reference for the ARINC 651 Integrated Modular Avionics (IMA) distributed architecture. The ADIRU has been designed to meet the commercial aviation market demands for low life cycle cost and high integrity fault detection, fault isolation, and redundancy management. The ADIRU's internal redundant resources provide quad channel redundancy that is one level higher than conventional triple redundant systems, allowing it to provide deferred maintenance capability. Robust partitioning, simple serial internal interfaces, and simple voting planes ensure that internal redundant components are properly utilized to provide high integrity system outputs. This relieves systems using it from having to perform their own redundancy management of the air data and inertial outputs from multiple sources required by conventional systems 相似文献
520.
P. Ubertini A. Bazzano L. Boccaccini N.A. Dipper L. Iafrate C. LaPadula M. Mastropietro R. Patriarca V. Polcaro M.L. Urciuoli 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1985,5(1):105-108
A new design of position sensitive spectroscopic proportional counter is described, for use in a balloon borne hard x-ray telescope. Initial position and spectral resolution data from a one-dimensional laboratory prototype are reported. With this device, the final telescope will have an angular resolution of better than 10 minutes of arc. 相似文献