Tolerance of anhydrobiotic eggs of the Tardigrade Ramazzottius varieornatus to extreme environments

Tardigrades are tiny (less than 1?mm in length) invertebrate animals that have the potential to survive travel to other planets because of their tolerance to extreme environmental conditions by means of a dry ametabolic state called anhydrobiosis. While the tolerance of adult tardigrades to extreme environments has been reported, there are few reports on the tolerance of their eggs. We examined the ability of hydrated and anhydrobiotic eggs of the tardigrade Ramazzottius varieornatus to hatch after exposure to ionizing irradiation (helium ions), extremely low and high temperatures, and high vacuum. We previously reported that there was a similar pattern of tolerance against ionizing radiation between hydrated and anhydrobiotic adults. In contrast, anhydrobiotic eggs (50% lethal dose; 1690 Gy) were substantially more radioresistant than hydrated ones (50% lethal dose; 509 Gy). Anhydrobiotic eggs also have a broader temperature resistance compared with hydrated ones. Over 70% of the anhydrobiotic eggs treated at either -196°C or +50°C hatched successfully, but all the hydrated eggs failed to hatch. After exposure to high-vacuum conditions (5.3×10(-4) Pa to 6.2×10(-5) Pa), the hatchability of the anhydrobiotic eggs was comparable to that of untreated control eggs.     

Improved algorithm for estimating pulse repetition intervals

This paper presents an improved algorithm for estimating pulse repetition intervals (PRIs) of an interleaved pulse train which consists of several independent radar signals with different PRIs. The original version of this algorithm is a complex-valued autocorrelation-like integral, which leads to a kind of PRI spectrum wherein the locations of the spectral peaks indicate the PRI values. The original algorithm, however, has a serious drawback in that it is vulnerable to timing jitter (PRI jitter). We analyze the cause of this vulnerability and propose an improved algorithm using overlapped PRI bins which have shifting time origins. The improved algorithm has proven to be quite effective in obtaining the PRI spectrum for jittered pulse trains, which enables detection of mean PRIs by thresholding     

Cosmic-ray electron spectrum above 100 GeV from PPB-BETS experiment in Antarctica

Cosmic-ray electrons have been observed in the energy region from 10 GeV to 1 TeV with the PPB-BETS by a long duration balloon flight using a Polar Patrol Balloon (PPB) in Antarctica. The observation was carried out for 13 days at an average altitude of 35 km in January 2004. The PPB-BETS detector is an imaging calorimeter composed of scintillating-fiber belts and plastic scintillators inserted between lead plates. In the study of cosmic-ray electrons, there have been some suggestions that high-energy electrons above 100 GeV are a powerful probe to identify nearby cosmic-ray sources and search for particle dark matter. In this paper, we present the energy spectrum of cosmic-ray electrons in the energy range from 100 GeV to 1 TeV at the top of atmosphere, and compare our spectrum with the results from other experiments.     

Depth dependency of neutron density produced by cosmic rays in the lunar subsurface

Depth dependency of neutrons produced by cosmic rays (CRs) in the lunar subsurface was estimated using the three-dimensional Monte Carlo particle and heavy ion transport simulation code, PHITS, incorporating the latest high energy nuclear data, JENDL/HE-2007. The PHITS simulations of equilibrium neutron density profiles in the lunar subsurface were compared with the measurement by Apollo 17 Lunar Neutron Probe Experiment (LNPE). Our calculations reproduced the LNPE data except for the 350–400 mg/cm² region under the improved condition using the CR spectra model based on the latest observations, well-tested nuclear interaction models with systematic cross section data, and JENDL/HE-2007.     

Effect of the space environment on the induction of DNA-repair related proteins and recovery from radiation damage.

Recovery of bacterial cells from radiation damage and the effects of microgravity were examined in an STS-79 Shuttle/Mir Mission-4 experiment using the extremely radioresistant bacterium Deinococcus radiodurans. The cells were irradiated with gamma rays before the space flight and incubated on board the Space-Shuttle. The survival of the wild type cells incubated in space increased compared with the ground controls, suggesting that the recovery of this bacterium from radiation damage was enhanced under microgravity. No difference was observed for the survival of radiosensitive mutant rec30 cells whether incubated in space or on the ground. The amount of DNA-repair related RecA protein induced under microgravity was similar to those of ground controls, however, induction of PprA protein, the product of a newly found gene related to the DNA repair mechanism of D. radiodurans, was enhanced under microgravity compared with ground controls.     

The structure of Kelvin–Helmholtz vortices with super-sonic flow

We have done two-dimensional simulations of the Kelvin–Helmholtz instability (KHI) with super-sonic flow using the CIP method. The linear analyses of a simple uniform density case show that the KHI cannot grow vigorously when the velocity jump is more than twice the sound speed (when the flow speed relative to the vortex is super-sonic). In this study, by situating a high density contrast across the shear layer, we set the flow in only one of the sides to be super-sonic and then show that the KHI does grow and rolls up a vortex. The formation of a shock is essential for the KHI vigorous growth and the structure of the vortex is strongly influenced by the shock geometry. The results should have substantial implications to velocity shear layer dynamics involving large density jump, such as planetary magnetospheric boundary layers.     

Photo-alteration of hydantoins against UV light and its relevance to prebiotic chemistry

Aqueous solutions of 5-substituted hydantoins were irradiated with ultraviolet (UV) light, to investigate their structural stability against UV radiation as well as the possible photolysis products. The photolysis products were identified and the degree of photolysis was measured using reversed-phase and ion-exchange high-performance liquid chromatography. Hydantoin (2,4-imidazolidinedione) was dominantly detected as a photolysis product of 5-substituted hydantoins. With hydrolysis of UV-irradiated 5-substituted hydantoins, glycine and alanine were dominantly detected. These experimental results are important for the prebiotic photochemistry of 5-substituted hydantoins in the formation of hydantoin since they have been detected in Solar System materials.     

Determining the Absolute Abundances of Natural Radioactive Elements on the Lunar Surface by the Kaguya Gamma-ray Spectrometer

The Kaguya gamma-ray spectrometer (KGRS) has great potential to precisely determine the absolute abundances of natural radioactive elements K, Th and U on the lunar surface because of its excellent spectroscopic performance. In order to achieve the best performance of the KGRS, it is important to know the spatial response function (SRF) that describes the directional sensitivity of the KGRS. The SRF is derived by a series of Monte Carlo simulations of gamma-ray transport in the sensor of the KGRS using the full-fledged simulation model of the KGRS, and is studied in detail. In this paper, the method for deriving absolute abundance of natural radioactive elements based on the SRF is described for the analysis of KGRS data, which is also applicable to any gamma-ray remote sensings. In the preliminary analysis of KGRS data, we determined the absolute abundances of K and Th on the lunar surface without using any previous knowledge of chemical information gained from Apollo samples, lunar meteorites and/or previous lunar remote sensings. The results are compared with the previous measurements and the difference and the correspondence are discussed. Future detailed analysis of KGRS data will provide new and more precise maps of K, Th and U on the lunar surface.     

Formation of bioorganic compounds in planetary atmospheres by cosmic radiation.

Simulated planetary atmospheres (mixtures of simple gases) were irradiated with high energy particles to simulate an action of cosmic rays. When a mixture of carbon monoxide, nitrogen and water was irradiated with 2.8-40 MeV protons, a wide variety of bioorganic compounds including amino acids, imidazole, and uracil were identified in the products. The amount of amino acids was proportional to the energy deposit to the system. Various kinds of simulated planetary atmospheres, such as "Titan type" and "Jovian type", were also irradiated with high energy protons, and gave amino acids in the hydrolyzed products. Since cosmic rays are a universal energy source in space, it was suggested that formation of bioorganic compounds in planetary atmospheres is inevitable in the course of cosmic evolution.     

The high precision gamma-ray spectrometer for lunar polar orbiter SELENE

The high precision gamma-ray spectrometer (GRS) is scheduled to be launched on the lunar polar orbiter of the SELENE mission in 2007. The GRS consists of a large Ge crystal as a main detector and massive bismuth germanate crystals as an anticoincidence detector. A Stirling cryocooler was adopted in cooling the Ge detector. The flight model of SELENE GRS has been completed and an energy resolution of 3.0 keV (FWHM) at 1.332 MeV has been achieved. The spectrometer aims to observe nuclear line gamma rays emitted from the lunar surface in a wide energy range from 100 keV to 12 MeV for one year and more to obtain chemical composition on the entire lunar surface. The gamma-ray data enable us to study lunar geoscience problems including crust and mantle composition, and volatile reservoirs at polar regions.