Osmoadaptive strategies of the archaeon Halococcus hamelinensis isolated from a hypersaline stromatolite environment

Biogenic stromatolites are sources of significant information on the evolution of microbial life. Despite their evolutionary significance, little is known about the mechanisms of osmoadaptation by microorganisms that comprise living stromatolites thriving in hypersaline environments. Osmoadaptive strategies for Halococcus hamelinensis, a novel halophilic archaeon recently isolated from living stromatolites in the hypersaline reaches of Shark Bay, were thus a particular interest in this study. To investigate the possibility of "salt-in-cytoplasm"-associated osmoadaptation for this archaeon, flame photometry studies were performed. From the results, it was evident that this halophilic archaeon did not accumulate intracellular K(+) ions when cells were exposed to either osmotic shock or conditions with gradual increments in salinity. These results were further supported by polymerase chain reaction (PCR) analyses where there was no evidence for the existence of homologous genes to an ATP-driven, high-affinity potassium uptake system in Halococcus hamelinensis. To identify an alternative salt adaptation mechanism associated with accumulation of compatible solutes for this archaeon, (1)H nuclear magnetic resonance (NMR) spectroscopy experiments were carried out. Results indicate that glycine betaine, trehalose, and glutamate are solutes likely to be involved in osmoregulation in this archeaon. Subsequent (1)H NMR analysis of cell extracts from this microorganism grown under various NaCl concentrations revealed that intracellular levels of glycine betaine increased with increasing concentrations of NaCl. This behavior of increasing glycine betaine concentration with increasing external NaCl is consistent with its identity as an osmolyte. In contrast, intracellular levels of trehalose were decreased in high concentrations of NaCl. This provides evidence that compatible solute accumulation appears to be the preferential salt regulation mechanism for this haloarchaeon, in contrast to the salt-in-cytoplasm strategy employed by many other halophilic archaea.     

High-energy gamma radiation of solar flares as an indicator of acceleration of energetic protons

Using the SONG detector onboard the CORONAS-F satellite, gamma-ray emission of high energies (>100 MeV) was recorded during four solar flares. In the sequential spectra of gamma rays the peculiarity caused by generation and decay of neutral pions was isolated, which made it possible to determine with a high accuracy the moments of appearance in the solar atmosphere of protons accelerated up to energies above 300 MeV.     

Creating Habitable Zones, at all Scales, from Planets to Mud Micro-Habitats, on Earth and on Mars

The factors that create a habitable planet are considered at all scales, from planetary inventories to micro-habitats in soft sediments and intangibles such as habitat linkage. The possibility of habitability first comes about during accretion, as a product of the processes of impact and volatile inventory history. To create habitability water is essential, not only for life but to aid the continual tectonic reworking and erosion that supply key redox contrasts and biochemical substrates to sustain habitability. Mud or soft sediment may be a biochemical prerequisite, to provide accessible substrate and protection. Once life begins, the habitat is widened by the activity of life, both by its management of the greenhouse and by partitioning reductants (e.g. dead organic matter) and oxidants (including waste products). Potential Martian habitats are discussed: by comparison with Earth there are many potential environmental settings on Mars in which life may once have occurred, or may even continue to exist. The long-term evolution of habitability in the Solar System is considered.     

The Swift GRB MIDEX mission

Swift is a first-of-its-kind multiwavelength transient observatory for γ-ray burst astronomy. It has the optimum capabilities for the next breakthroughs in determining the origin of γ-ray bursts and their afterglows, as well as for using bursts to probe the early Universe. Swift will also monitor the soft gamma repeaters and perform the first sensitive hard X-ray survey of the sky. The mission is being developed by an international collaboration and consists of three instruments, the Burst Alert Telescope (BAT), the X-ray Telescope (XRT), and the Ultraviolet and Optical Telescope (UVOT). The BAT, a wide-field γ-ray detector, will detect >100 γ-ray bursts per year with a sensitivity 5× that of BATSE. The sensitive narrow-field XRT and UVOT will be autonomously slewed to the burst location within 20–70 s to determine 0.3–5.0″ positions and perform optical, UV, and X-ray spectrophotometry. Strong education/public outreach and follow-up programs will help to engage the public and the astronomical community. Swift launch is planned for late 2004.     

Reflections on the quest for international cooperation

Changed political objectives, straitened economic circumstances and an altered balance of capability and expertise in space endeavours have together produced more discussion of the need for international cooperation than ever before, but the meaning of the term has subtly shifted. Insisting on US ‘leadership’ is self-defeating; what is instead firmly desired by the USA's potential partners is a balanced relationship in which each nation makes an identifiable contribution and takes the lead in at least one of a package of projects. If this is going to happen, there must be an international forum or framework in which projects can be discussed and agreed. This in turn requires initiative at governmental level.     

Development of a high pressure-differential turbine blade cooling system

A convective-film system of high pressure-differential turbine blade cooling is presented. The results of calculating the thermal-hydraulic blade state using the KW3D software are given.     

Single Degenerate Models for Type Ia Supernovae: Progenitor’s Evolution and Nucleosynthesis Yields

We review how the single degenerate models for Type Ia supernovae (SNe Ia) works. In the binary star system of a white dwarf (WD) and its non-degenerate companion star, the WD accretes either hydrogen-rich matter or helium and undergoes hydrogen and helium shell-burning. We summarize how the stability and non-linear behavior of such shell-burning depend on the accretion rate and the WD mass and how the WD blows strong wind. We identify the following evolutionary routes for the accreting WD to trigger a thermonuclear explosion. Typically, the accretion rate is quite high in the early stage and gradually decreases as a result of mass transfer. With decreasing rate, the WD evolves as follows: (1) At a rapid accretion phase, the WD increase its mass by stable H burning and blows a strong wind to keep its moderate radius. The wind is strong enough to strip a part of the companion star’s envelope to control the accretion rate and forms circumstellar matter (CSM). If the WD explodes within CSM, it is observed as an “SN Ia-CSM”. (X-rays emitted by the WD are absorbed by CSM.) (2) If the WD continues to accrete at a lower rate, the wind stops and an SN Ia is triggered under steady-stable H shell-burning, which is observed as a super-soft X-ray source: “SN Ia-SSXS”. (3) If the accretion continues at a still lower rate, H shell-burning becomes unstable and many flashes recur. The WD undergoes recurrent nova (RN) whose mass ejection is smaller than the accreted matter. Then the WD evolves to an “SN Ia-RN”. (4) If the companion is a He star (or a He WD), the accretion of He can trigger He and C double detonations at the sub-Chandrasekhar mass or the WD grows to the Chandrasekhar mass while producing a He-wind: “SN Ia-He CSM”. (5) If the accreting WD rotates quite rapidly, the WD mass can exceed the Chandrasekhar mass of the spherical WD, which delays the trigger of an SN Ia. After angular momentum is lost from the WD, the (super-Chandra) WD contracts to become a delayed SN Ia. The companion star has become a He WD and CSM has disappeared: “SN Ia-He WD”. We update nucleosynthesis yields of the carbon deflagration model W7, delayed detonation model WDD2, and the sub-Chandrasekhar mass model to provide some constraints on the yields (such as Mn) from the comparison with the observations. We note the important metallicity effects on ⁵⁸Ni and ⁵⁵Mn.     

Crustal S-Wave Velocity from Apparent Incidence Angles: A Case Study in Preparation for InSight

Retrieval of crustal structure and thickness of Mars is among the main goals of InSight. Here we investigate which constraints on the crust at the landing site can be provided by apparent P-wave incidence angles derived from P-receiver functions. We consider receiver functions for six different Mars models, calculated from synthetic seismograms generated via Instaseis from the Green’s function databases of the Marsquake Service, in detail. To allow for a larger range of crustal thicknesses and structures, we additionally analyze data from five broad-band stations across Central Europe. We find that the likely usable epicentral distance range for P-wave receiver functions on Mars lies between \(35^{\circ}\) and the core shadow, and can be extended to more than \(150^{\circ}\) by also using the PP-phase. Comparison to models for the spatial distribution of Martian seismicity indicates that sufficient seismicity should occur within the P-wave distance range around InSight within the nominal mission duration to allow for the application of our method. Apparent P-wave incidence angles are derived from the amplitudes of vertical and radial receiver functions at the P-wave onset within a range of period bands, up to 120 s. The apparent incidence angles are directly related to apparent S-wave velocities, which are inverted for the subsurface S-wave velocity structure via a grid search. The veracity of the forward calculated receiver functions and apparent S-wave velocities is ensured by benchmarking various algorithms against the Instaseis synthetics. Results indicate that apparent S-wave velocity curves provide valuable constraints on crustal thickness and structure, even without any additional constraints, and considering the location uncertainty and limited data quantity of InSight. S-wave velocities in the upper half of the crust are constrained best, but if reliable measurements at long periods are available, the curves also provide constraints down to the uppermost mantle. Besides, it is demonstrated that the apparent velocity curves can differentiate between crustal velocity models that are indistinguishable by other methods.     

Cosmological Distance Indicators

We review three distance measurement techniques beyond the local universe: (1) gravitational lens time delays, (2) baryon acoustic oscillation (BAO), and (3) HI intensity mapping. We describe the principles and theory behind each method, the ingredients needed for measuring such distances, the current observational results, and future prospects. Time-delays from strongly lensed quasars currently provide constraints on \(H_{0}\) with \(<4\%\) uncertainty, and with \(1\%\) within reach from ongoing surveys and efforts. Recent exciting discoveries of strongly lensed supernovae hold great promise for time-delay cosmography. BAO features have been detected in redshift surveys up to \(z\lesssim0.8\) with galaxies and \(z\sim2\) with Ly-\(\alpha\) forest, providing precise distance measurements and \(H_{0}\) with \(<2\%\) uncertainty in flat \(\Lambda\)CDM. Future BAO surveys will probe the distance scale with percent-level precision. HI intensity mapping has great potential to map BAO distances at \(z\sim0.8\) and beyond with precisions of a few percent. The next years ahead will be exciting as various cosmological probes reach \(1\%\) uncertainty in determining \(H_{0}\), to assess the current tension in \(H_{0}\) measurements that could indicate new physics.