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Abundance and diversity of microbial inhabitants in European spacecraft-associated clean rooms 总被引:1,自引:0,他引:1
M Stieglmeier P Rettberg S Barczyk M Bohmeier R Pukall R Wirth C Moissl-Eichinger 《Astrobiology》2012,12(6):572-585
The determination of the microbial load of a spacecraft en route to interesting extraterrestrial environments is mandatory and currently based on the culturable, heat-shock-surviving portion of microbial contaminants. Our study compared these classical bioburden measurements as required by NASA's and ESA's guidelines for the microbial examination of flight hardware, with molecular analysis methods (16S rRNA gene cloning and quantitative PCR) to further develop our understanding of the diversity and abundance of the microbial communities of spacecraft-associated clean rooms. Three samplings of the Herschel Space Observatory and its surrounding clean rooms were performed in two different European facilities. Molecular analyses detected a broad diversity of microbes typically found in the human microbiome with three bacterial genera (Staphylococcus, Propionibacterium, and Brevundimonas) common to all three locations. Bioburden measurements revealed a low, but heterogeneous, abundance of spore-forming and other heat-resistant microorganisms. Total cell numbers estimated by quantitative real-time PCR were typically 3 orders of magnitude greater than those determined by viable counts, which indicates a tendency for traditional methods to underestimate the extent of clean room bioburden. Furthermore, the molecular methods allowed the detection of a much broader diversity than traditional culture-based methods. 相似文献
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为了对发动机燃烧不稳定性进行被动控制,利用亥姆赫兹声学共振器原理,设计一种声学阀门,当一个管道侧面安装的亥姆赫兹共振器的空腔壁面是柔软的时候,声学阀门的性能与频率就没有很强的关系,从而实现由于热声学不稳定带来的压力和热释放导致的不稳定燃烧进行被动控制。阀门的功能是让声音通过,但它必须阻止时间平均意义上的流动。本文对带有这种装置的热声学特征根问题给出数值解。结果显示声学阀门对燃烧室内的驻波结构造成很大改变,进而可以消除不稳定的特征根模态。只要阀门具备足够的尺寸,这种效果可以在任意的线性火焰声学特性中广泛实现。 相似文献
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P Tsou DE Brownlee CP McKay AD Anbar H Yano K Altwegg LW Beegle R Dissly NJ Strange I Kanik 《Astrobiology》2012,12(8):730-742
Abstract Life Investigation For Enceladus (LIFE) presents a low-cost sample return mission to Enceladus, a body with high astrobiological potential. There is ample evidence that liquid water exists under ice coverage in the form of active geysers in the "tiger stripes" area of the southern Enceladus hemisphere. This active plume consists of gas and ice particles and enables the sampling of fresh materials from the interior that may originate from a liquid water source. The particles consist mostly of water ice and are 1-10?μ in diameter. The plume composition shows H(2)O, CO(2), CH(4), NH(3), Ar, and evidence that more complex organic species might be present. Since life on Earth exists whenever liquid water, organics, and energy coexist, understanding the chemical components of the emanating ice particles could indicate whether life is potentially present on Enceladus. The icy worlds of the outer planets are testing grounds for some of the theories for the origin of life on Earth. The LIFE mission concept is envisioned in two parts: first, to orbit Saturn (in order to achieve lower sampling speeds, approaching 2 km/s, and thus enable a softer sample collection impact than Stardust, and to make possible multiple flybys of Enceladus); second, to sample Enceladus' plume, the E ring of Saturn, and the Titan upper atmosphere. With new findings from these samples, NASA could provide detailed chemical and isotopic and, potentially, biological compositional context of the plume. Since the duration of the Enceladus plume is unpredictable, it is imperative that these samples are captured at the earliest flight opportunity. If LIFE is launched before 2019, it could take advantage of a Jupiter gravity assist, which would thus reduce mission lifetimes and launch vehicle costs. The LIFE concept offers science returns comparable to those of a Flagship mission but at the measurably lower sample return costs of a Discovery-class mission. Key Words: Astrobiology-Habitability-Enceladus-Biosignatures. Astrobiology 12, 730-742. 相似文献
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本文分析了多微(MUMI)处理机系统的可性。在多微处理机中,分配给一些模块固定不变的任务,有的起计算处理机的作用,有的起 l/I 处理机的作用。每台处理机都有局部存贮器,能够对任何期的任务保存指令和块。此外,它们共享一个公用存贮器。所有模块都由受处理机控制的冗余多路总线互连;另一个处理机作整个系统的主处理机之用。 相似文献
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对两种自适应检测方案,Kelly的广义似然比检验(GLRT)和平均电平自适应检测(MLAD),进行了比较,两种方案检测性能PD的预测量在输入噪声为时间独立但空间相关的零均值复高斯随机变量以及有两个信号幅度呈端利分布的假设条件下进行的。PD是作为虚警概率,输放通道数,每个通道的独立采样数以及匹配滤波器输出信号噪声功率比等参量的函数来计算的。本文的分析表明:GLRT比MLAD有更好的检测性能。当使用较 相似文献
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