Cave biosignature suites: microbes,minerals, and Mars

Earth's subsurface offers one of the best possible sites to search for microbial life and the characteristic lithologies that life leaves behind. The subterrain may be equally valuable for astrobiology. Where surface conditions are particularly hostile, like on Mars, the subsurface may offer the only habitat for extant lifeforms and access to recognizable biosignatures. We have identified numerous unequivocally biogenic macroscopic, microscopic, and chemical/geochemical cave biosignatures. However, to be especially useful for astrobiology, we are looking for suites of characteristics. Ideally, "biosignature suites" should be both macroscopically and microscopically detectable, independently verifiable by nonmorphological means, and as independent as possible of specific details of life chemistries--demanding (and sometimes conflicting) criteria. Working in fragile, legally protected environments, we developed noninvasive and minimal impact techniques for life and biosignature detection/characterization analogous to Planetary Protection Protocols. Our difficult field conditions have shared limitations common to extraterrestrial robotic and human missions. Thus, the cave/subsurface astrobiology model addresses the most important goals from both scientific and operational points of view. We present details of cave biosignature suites involving manganese and iron oxides, calcite, and sulfur minerals. Suites include morphological fossils, mineral-coated filaments, living microbial mats and preserved biofabrics, 13C and 34S values consistent with microbial metabolism, genetic data, unusual elemental abundances and ratios, and crystallographic mineral forms.     

Efficacy of Doppler ultrasound [correction of utrasound] for screening symptoms of decompression sickness during simulated extravehicular activities

Doppler ultrasound is frequently used for monitoring circulating microbubbles during decompression to assess the symptoms of Decompression Sickness (DCS). This analysis was carried out to evaluate its effectiveness for screening symptoms of DCS during simulated extravehicular activities (EVA). The information from various hypobaric chamber studies carried out at the NASA Johnson Space Center, Houston, TX was used in this analysis (n = 516). The circulating microbubbles were detected in the precordial area in 42% (218/516), and symptoms were reported in 16% (81/516) of these exposures. The accuracy of Doppler-detectable bubbles (Spencer grades) on all symptoms of DCS was examined by calculating measures of sensitivity and specificity. The efficacy of Doppler as a screening device was examined by calculating their positive predictive value (PPV) and negative predictive value (NPV). The results of these analyses indicated that the sensitivity of Doppler decreased, and the PPV increased with higher Spencer grades. However, the likelihood of detecting true negative cases (NPV) was consistently higher with all bubble grades. Due to the high false-positive rate and low prior probabilities of the risk of DCS, Doppler was found to be more useful to identify those who did not develop DCS, than to detect positive cases of DCS in the simulated EVA exposures.     

Main medical results of extended flights on space station Mir in 1986-1990

During 1986-1990 seven prime spacecrews (16 cosmonauts) have flown on-board the Mir orbital complex. The longest space mission duration was 366 days The principal objectives of the medical tasks were the maintenance of good health and performance of the spacecrews and conducting medical research programs which included study of the cardiovascular, motor, endocrine, blood, immune, and metabolic systems. Results obtained point to the ability of humans to readily adapt to a year-long stay in space and maintain good health and performance. Readaptation had a similar course as after other previous long-term space flights of up to 8 months in duration. Primary body system changes were not qualitatively different from findings after flights aboard the Salyut 6 and 7 space stations. In this case, during and after an 11-12 month flight, body system alterations were even less severe which was a result of adequate countermeasure use, their systematic and creative employment and maintenance of required environments to support life and work in space.     

Electrostatic Noise Spectrum at the Electron Cyclotron Frequency and Electromagnetic Emission in the Inhomogeneous Plasma of the Topside Ionosphere

Measurements of the wave emission of the topside ionosphere made onboard the APEX satellite using the electric component of the wave field in the 0.1–10 MHz frequency band are presented. At middle latitudes a wave intensity decrease was observed in the broad-band spectrum of the electrostatic noise at the electron cyclotron frequency. It is shown that a break in the spectrum of electrostatic modes at the electron cyclotron frequency (the absence of the plasma eigen-frequencies) may be a cause of the observed effect. The increase of the intensity at the electron cyclotron frequency in the ionospheric trough and at latitudes above the trough region as compared to middle latitudes may be explained by the capture by plasma irregularities of the electromagnetic emission of the auroral electron fluxes.__________Translated from Kosmicheskie Issledovaniya, Vol. 43, No. 3, 2005, pp. 201–208.Original Russian Text Copyright © 2005 by Izhovkina, Prutensky, Pulinets, Kiraga, Klos, Rothkael.     

A new method of determining the spectral index for protons in the energy range about 10<Superscript>13</Superscript> eV

We consider a relationship between the difference in spectral indices of the spectra of single hadrons and all hadrons (_sngl – _h) and the difference in the indices of the spectra of galactic cosmic ray (GCR) protons and nuclei. It is demonstrated that at the mountain level the ratio (_p – _Z)/(_sngl – _h) is always larger than unity, if (_sngl – _h) > 0.1. From the experimental value _sngl – _h = 0.4 ± 0.05 we derive that, in the vicinity of E = 10 TeV, _p – _Z 0.49 ± 0.06 , i.e., _p 3.09 ± 0.06.__________Translated from Kosmicheskie Issledovaniya, Vol. 43, No. 2, 2005, pp. 83–87.Original Russian Text Copyright © 2005 by Grigorov, Tolstaya.     

Advantages and disadvantages of prizes in a portfolio of financial incentives for space activities

The National Aeronautics and Space Administration (NASA) has proposed to use financial prizes to encourage innovation in space technology. Public debate about the use of prizes questions their effectiveness, the role of government compared with the private sector in administering prizes—for example, the Ansari X-Prize for human suborbital flight was privately funded and administered—and other issues that are likely to influence the success of this approach.     

Changes in chromatin and nucleic acids in rat tissues after two-week spaceflight

The quantitative changes in nucleic acids and chromatin breakdown were followed in blood, thymus and spleen in rats after 14 day flights on board the biosatellites Cosmos-1887 and Cosmos-2044. Quantitative nucleic acid changes within 8-11 h after landing were only mild, most statistically non-significant. An analysis at 48 h after landing showed a marked decrease in a total content of DNA and RNA in spleen and thymus. Within 8-11 h after landing, the symptoms of chromatin breakdown were found as is seen in an increased concentration of its fragments-polydeoxyribonucleotides. The obtained results show that a partial adaptation to microgravity occurs up to flight day 14 in lymphoid organs. Adaptation is accompanied with a reappearing of the sensitive cells. Their chromatin breaks down, then, in a final phase of flight due to hypergravity stress manifesting itself by a temporary increase in polydeoxyribonucleotide concentration several hours after landing. The results are discussed in relation to the changes in chosen parameters after shorter or more prolonged flights.     

In-situ resource utilization technologies for Mars life support systems.

The atmosphere of Mars has many of the ingredients that can be used to support human exploration missions. It can be "mined" and processed to produce oxygen, buffer gas, and water, resulting in significant savings on mission costs. The use of local materials, called ISRU (for in-situ resource utilization), is clearly an essential strategy for a long-term human presence on Mars from the standpoints of self-sufficiency, safety, and cost. Currently a substantial effort is underway by NASA to develop technologies and designs of chemical plants to make propellants from the Martian atmosphere. Consumables for life support, such as oxygen and water, will probably benefit greatly from this ISRU technology development for propellant production. However, the buffer gas needed to dilute oxygen for breathing is not a product of a propellant production plant. The buffer gas needs on each human Mars mission will probably be in the order of metric tons, primarily due to losses during airlock activity. Buffer gas can be separated, compressed, and purified from the Mars atmosphere. This paper discusses the buffer gas needs for a human mission to Mars and consider architectures for the generation of buffer gas including an option that integrates it to the propellant production plant.     

Prompt effects of solar wind variations on the inner magnetosphere and midlatitude ionosphere

It is well known that the solar wind can significantly affect high-latitude ionospheric dynamics. However, the effects of the solar wind on the middle- and low-latitude ionosphere are much less studied. In this paper, we report observations that large perturbations in the middle- and low-latitude ionosphere are well correlated with solar wind variations. In one event, a significant (20–30%) decrease of the midlatitude ionospheric electron density over a large latitudinal range was related to a sudden drop in the solar wind pressure and a northward turning of the interplanetary magnetic field, and the density decrease became larger at lower latitudes. In another event, periodic perturbations in the dayside equatorial ionospheric E × B drift and electrojet were closely associated with variations in the interplanetary electric field. Since the solar wind is always changing with time, it can be a very important and common source of ionospheric perturbations at middle- and low-latitudes. The relationship between solar wind variations and significant ionospheric perturbations has important applications in space weather.     

The atmospheric radiation response to solar-particle-events.

High-energy solar particles, produced in association with solar flares and coronal mass ejections, occasionally bombard the earth's atmosphere. resulting in radiation intensities additional to the background cosmic radiation. Access of these particles to the earth's vicinity during times of geomagnetic disturbances are not adequately described by using static geomagnetic field models. These solar fluxes are also often distributed non uniformly in space, so that fluxes measured by satellites obtained at great distances from the earth and which sample large volumes of space around the earth cannot be used to predict fluxes locally at the earth's surface. We present here a method which uses the ground-level neutron monitor counting rates as adjoint sources of the flux in the atmosphere immediately above them to obtain solar-particle effective dose rates as a function of position over the earth's surface. We have applied this approach to the large September 29-30, 1989 ground-level event (designated GLE 42) to obtain the magnitude and distribution of the solar-particle effective dose rate from an atypically large event. The results of these calculations clearly show the effect of the softer particle spectra associated with solar particle events, as compared with galactic cosmic rays, results in a greater sensitivity to the geomagnetic field, and, unlike cosmic rays, the near-absence of a "knee" near 60 degrees geomagnetic latitude.