Extreme dryness and DNA-protein cross-links.

Exposure of fungal conidia (Aspergillus ochraceus) or spores of Bacillus subtilis to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered water activity results in an increasing number of cross-links between DNA and proteins. In fungal conidia these cross-links are detected after selective iodination (125 J) of the DNA-bound proteins followed by gel electrophoresis and subsequent autoradiography. Another approach is the labelling of DNA with 32P by means of nick translation and the detection of differences in the electrophoretic mobility of DNA before and after digestion with proteinase K of proteins bound to DNA.     

Infrared spectroscopy of organics of planetological interest at low temperatures.

In the context of prebiotic chemistry in space, some of the outer planetary objects display H, C, N and O rich chemistry similar to the one in the biosphere of Earth. Of particular interest are Saturn's moon, Titan; Neptune's moon, Triton; and Pluto where extreme cold conditions prevail. Identifications of chemical species on these objects (surfaces and atmospheres) is essential to a better understanding of the radiation induced chemical reactions occurring thereon. There have been several ground based observations of these planetary objects in the infrared windows from 1 to 2.5 micrometers. Voyager also provided spectra in the thermal infrared (6 to 50 micrometers) region. Interpretation of these data require laboratory infrared spectra of relevant species under the temperature conditions appropriate to these objects. The results of some of these studies carried out in our laboratory and elsewhere and their impact on the analyses of the observed data will be summarized.     

Reduced lymphocyte activation in space: role of cell-substratum interactions.

We investigated the effect of substratum adhesiveness on stimulated lymphocyte blastogenesis by reducing and blocking cell adhesion with poly (2-hydroxyethyl methacrylate) (poly-HEMA) in a simple on-ground system. Cells grown on medium-thick and thick poly-HEMA films were rounded in shape and displayed no signs of spreading. By contrast, on tissue culture plastic and very thin poly-HEMA films, they showed clear signs of spreading. The mitogenic response of lymphocytes grown on thick poly-HEMA films was reduced by up to 68% of the control (tissue culture plastic). Interferon-gamma production was near zero when the cells were grown on the least adhesive substratum. On uncoated plastic, activated lymphocytes subjected to high gravity (20g) exhibited an increased proliferation rate (40%) compared with 1g. By contrast, on poly-HEMA, high gravity did not improve lymphocyte responsiveness. These results show that activated lymphocytes need to anchor and spread prior to achieving an optimal proliferation response. We conclude that decreased lymphocyte adhesion could contribute to the depressed in vitro lymphocyte responsiveness found in the microgravity conditions of space flight.     

Several recent findings concerning the dynamics of the Earth's magnetotail

Several recent results concerning the nature of the Earth's magnetotail are briefly reviewed. These observational findings include: (1) the three-dimensional character of the plasma sheet via a comprehensive survey of proton bulk flows, (2) a region of earthward flowing plasmas at the interfaces of the plasma sheet and magnetotail lobes during magnetic substorm recovery, and (3) the signature of electrostatic acceleration for protons within the jetting plasmas from magnetotail fireballs.Proceedings of the Symposium on Solar Terrestrial Physics held in Innsbruck, May–June 1978.     

Why space physics needs to go beyond the MHD box

Magnetohydrodynamic (MHD) theory has been used in space physics for more than forty years, yet many important questions about space plasmas remain unanswered. We still do not understand how the solar wind is accelerated, how mass, momentum and energy are transported into the magnetosphere and what mechanisms initiate substorms. Questions have been raised from the beginning of the space era whether MHD theory can describe correctly space plasmas that are collisionless and rarely in thermal equilibrium. Ideal MHD fluids do not induce electromotive force, hence they lose the capability to interact electromagnetically. No currents and magnetic fields are generated, rendering ideal MHD theory not very useful for space plasmas. Observations from the plasma sheet are used as examples to show how collisionless plasmas behave. Interpreting these observations using MHD and ideal MHD concepts can lead to misleading conclusions. Notably, the bursty bulk flows (BBF) with large mean velocities left(〈 v 〉≥400 km s right) that have been interpreted previously as E×B flows are shown to involve much more complicated physics. The sources of these nonvanishing 〈 v 〉 events, while still not known, are intimately related to mechanisms that create large phase space gradients that include beams and acceleration of ions to MeV energies. The distributions of these nonvanishing 〈 v 〉 events are associated with large amplitude variations of the magnetic field at frequencies up to and exceeding the local Larmor frequency where MHD theory is not valid. Understanding collisionless plasma dynamics such as substorms in the plasma sheet requires the self-consistency that only kinetic theory can provide. Kinetic modeling is still undergoing continual development with many studies limited to one and two dimensions, but there is urgent need to improve these models as more and more data show kinetic physics is fundamentally important. Only then will we be able to make progress and obtain a correct picture of how collisionless plasmas work in space.     

Microbial utilisation of natural organic wastes

The waste management strategy for the future should meet the benefits of humanity safety, respect principals of planet ecology, and compatibility with other habitability systems. For these purpose the waste management technologies, relevant to application of the biodegradation properties of bacteria are of great value. The biological treatment method is based upon the biodegradation of organic substances by various microorganisms. The advantage of the biodegradation waste management in general: it allows to diminish the volume of organic wastes, the biological hazard of the wastes is controlled, and this system may be compatible with the other systems. The objectives of our study were: to evaluate effectiveness of microbial biodegradation of non-pretreated substrate, to construct phneumoautomatic digester for organic wastes biodegradation, and to study microbial characteristics of active sludge samples used as inoculi in biodegradation experiment. The technology of vegetable wastes treatment was elaborated in IBMP and BMSTU. For this purpose the special unit was created where the degradation process is activated by enforced reinvention of portions of elaborated biogas into digester. This technology allows to save energy normally used for electromechanical agitation and to create optimal environment for anaerobic bacteria growth. The investigations were performed on waste simulator, which imitates physical and chemical content of food wastes calculated basing on the data on food wastes of moderate Russian city. The volume of created experimental sample of digester is 40 l. The basic system elements of device are digesters, gas receiver, remover of drops and valve monitoring and thermal control system. In our testing we used natural food wastes to measure basic parameters and time of biodegradation process. The diminution rate of organic gained 76% from initial mass taking part within 9 days of fermentation. The biogas production achieved 46 l per 1 kg of substrate. The microbial studies of biodegradation process revealed following peculiarities: (i) gradual quantitative increasing of Lactobacillus sp. (from 10(3) to 10(5) colony forming units (CFU) per ml), (ii) activation of Clostridia sp. (from 10(2) to 10(4)CFU/ml), (iii) elimination of aerobic conventional pathogens (Enterobacteriaceae sp., Protea sp., staphylococci). The obtained results allow to evaluate effectiveness of proposed technology and to determine the leading role of lactobacilli and clostridia in process of natural wastes biodegradation. Our further investigations shall further be concentrated on creation of artificial inoculi for launching of food wastes biodegradation. These inoculi will include active and adapted strains of clostridia and lactobacilli.     

Daytime VHF amplitude scintillations recorded at an Indian low-latitude station,Waltair (17.7°N, 83.3°E) during 1997–2003

In this research, it is presented the daytime amplitude scintillations recorded at VHF frequency (244 MHz) at an Indian low-latitude station, Waltair (17.7°N, 83.3°E) during seven continuous years (1997–2003). Contrary to the nighttime scintillation seasonal trends, the occurrence of daytime scintillations maximizes during summer followed by winter and the equinox seasons. The fade depths, scintillation indices and the patch durations of daytime scintillations are meager when compared with their nighttime counterparts. A co-located digital high frequency (HF) ionosonde radar confirms the presence of sporadic (Es) layers when daytime scintillations are observed. The presence of daytime scintillations is evident when the critical frequency of the Es-layer (foEs) is ≥4 MHz and Es-layers are characterized by a highly diffuse range spread Es echoes as can be seen on ionograms. It is surmised that the gradient drift instability (GDI) seems to be the possible mechanism for the generation of these daytime scintillations. It is quite likely that the spread Es-F-layer coupling is done through polarization electric fields (Ep) that develop inside the destabilized patches of sporadic E layers, which are mapped up to the F region along the field lines as to initiate the daytime scintillations through the GDI mechanism. Further, the presence of additional stratification of ionosphere F-layer, popularly known as the F3-layer, is observed on ionograms once the Es-layers and daytime scintillations are ceased.     

Regolith X-Ray Imaging Spectrometer (REXIS) Aboard the OSIRIS-REx Asteroid Sample Return Mission

The Regolith X-ray Imaging Spectrometer (REXIS) is the student collaboration experiment proposed and built by an MIT-Harvard team, launched aboard NASA’s OSIRIS-REx asteroid sample return mission. REXIS complements the scientific investigations of other OSIRIS-REx instruments by determining the relative abundances of key elements present on the asteroid’s surface by measuring the X-ray fluorescence spectrum (stimulated by the natural solar X-ray flux) over the range of energies 0.5 to 7 keV. REXIS consists of two components: a main imaging spectrometer with a coded aperture mask and a separate solar X-ray monitor to account for the Sun’s variability. In addition to element abundance ratios (relative to Si) pinpointing the asteroid’s most likely meteorite association, REXIS also maps elemental abundance variability across the asteroid’s surface using the asteroid’s rotation as well as the spacecraft’s orbital motion. Image reconstruction at the highest resolution is facilitated by the coded aperture mask. Through this operation, REXIS will be the first application of X-ray coded aperture imaging to planetary surface mapping, making this student-built instrument a pathfinder toward future planetary exploration. To date, 60 students at the undergraduate and graduate levels have been involved with the REXIS project, with the hands-on experience translating to a dozen Master’s and Ph.D. theses and other student publications.     

Comparison of vertical E × B drift velocities and ground-based magnetometer observations of DELTA H in the low latitude under geomagnetically disturbed conditions

In the present work, we analyzed the daytime vertical E × B drift velocities obtained from Jicamarca Unattended Long-term Ionosphere Atmosphere (JULIA) radar and ΔH component of geomagnetic field measured as the difference between the magnitudes of the horizontal (H) components between two magnetometers deployed at two different locations Jicamarca, and Piura in Peru for 22 geomagnetically disturbed events in which either SC has occurred or Dst_max < ?50 nT during the period 2006–2011. The ΔH component of geomagnetic field is measured as the differences in the magnitudes of horizontal H component between magnetometer placed directly on the magnetic equator and one displaced 6–9° away. It will provide a direct measure of the daytime electrojet current, due to the eastward electric field. This will in turn gives the magnitude of vertical E × B drift velocity in the F region. A positive correlation exists between peak values of daytime vertical E × B drift velocity and peak value of ΔH for the three consecutive days of the events. It was observed that 45% of the events have daytime vertical E × B drift velocity peak in the magnitude range 10–20 m/s and 20–30 m/s and 20% have peak ΔH in the magnitude range 50–60 nT and 80–90 nT. It was observed that the time of occurrence of the peak value of both the vertical E × B drift velocity and the ΔH have a maximum (40%) probability in the same time range 11:00–13:00 LT. We also investigated the correlation between E × B drift velocity and D_st index and the correlation between delta H and D_st index. A strong positive correlation is found between E × B drift and D_st index as well as between delta H and D_st Index. Three different techniques of data analysis – linear, polynomial (order 2), and polynomial (order 3) regression analysis were considered. The regression parameters in all the three cases were calculated using the Least Square Method (LSM), using the daytime vertical E × B drift velocity and ΔH. A formula was developed which indicates the relationship between daytime vertical E × B drift velocity and ΔH, for the disturbed periods. The E × B drift velocity was then evaluated using the formulae thus found for the three regression analysis and validated for the ‘disturbed periods’ of 3 selected events. The E × B drift velocities estimated by the three regression analysis have a fairly good agreement with JULIA radar observed values under different seasons and solar activity conditions. Root Mean Square (RMS) errors calculated for each case suggest that polynomial (order 3) regression analysis provides a better agreement with the observations from among the three.