Genomic instability and tumorigenic induction in immortalized human bronchial epithelial cells by heavy ions

Carcinogenesis is postulated to be a progressive multistage process characterized by an increase in genomic instability and clonal selection with each mutational event endowing a selective growth advantage. Genomic instability as manifested by the amplification of specific gene fragments is common among tumor and transformed cells. In the present study, immortalized human bronchial (BEP2D) cells were irradiated with graded doses of either 1GeV/nucleon ⁵⁶Fe ions or 150 keV/μm alpha particles. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Tumorigenic cells showed neither ras mutations nor deletion in the p16 tumor suppressor gene. In contrast, they harbored mutations in the p53 gene and over-expressed cyclin D1. Genomic instability among transformed cells at various stage of the carcinogenic process was examined based on frequencies of PALA resistance. Incidence of genomic instability was highest among established tumor cell lines relative to transformed, non-tumorigenic and control cell lines. Treatment of BEP2D cells with a 4 mM dose of the aminothiol WR-1065 significantly reduced their neoplastic transforming response to ⁵⁶Fe particles. This model provides an opportunity to study the cellular and molecular mechanisms involved in malignant transformation of human epithelial cells by heavy ions.     

Innovations for competitiveness: European views on “better-faster-cheaper”

The paper elaborates on “ lessons learned” from two recent ESA workshops, one focussing on the role of Innovation in the competitiveness of the space sector and the second on technology and engineering aspects conducive to better, faster and cheaper space programmes. The paper focuses primarily on four major aspects, namely:

1. a) the adaptations of industrial and public organisations to the global market needs;

2. b) the understanding of the bottleneck factors limiting competitiveness;

3. c) the trends toward new system architectures and new engineering and production methods;

4. d) the understanding of the role of new technology in the future applications.

Under the pressure of market forces and the influence of many global and regional players, applications of space systems and technology are becoming more and more competitive. It is well recognised that without major effort for innovation in industrial practices, organisations, R&D, marketing and financial approaches the European space sector will stagnate and loose its competence as well as its competitiveness. It is also recognised that a programme run according to the “better, faster, cheaper” philosophy relies on much closer integration of system design, development and verification, and draws heavily on a robust and comprehensive programme of technology development, which must run in parallel and off-line with respect to flight programmes.

A company's innovation capabilities will determine its future competitive advantage (in time, cost, performance or value) and overall growth potential. Innovation must be a process that can be counted on to provide repetitive, sustainable, long-term performance improvements. As such, it needs not depend on great breakthroughs in technology and concepts (which are accidental and rare). Rather, it could be based on bold evolution through the establishment of know-how, application of best practices, process effectiveness and high standards, performance measurement, and attention to customers and professional marketing. Having a technological lead allows industry to gain a competitive advantage in performance, cost and opportunities. Instrumental to better competitiveness is an R&D effort based on the adaptation of high technology products, capable of capturing new users, increasing production, decreasing the cost and delivery time and integrating high level of intelligence, information and autonomy. New systems will have to take in to account from the start what types of technologies are being developed or are already available in other areas outside space, and design their system accordingly. The future challenge for “faster, better, cheaper” appears to concern primarily “cost-effective”, performant autonomous spacecraft, “cost-effective”, reliable launching means and intelligent data fusion technologies and robust software serving mass- market real time services, distributed via EHF bands and Internet.

In conclusion, it can be noticed that in the past few years new approaches have considerably enlarged the ways in which space missions can be implemented. They are supported by true innovations in mission concepts, system architecture, development and technologies, in particular for the development of initiatives based on multi-mission mini-satellites platforms for communication and Earth observation missions. There are also definite limits to cost cutting (such as lowering heads counts and increasing efficiency), and therefore the strategic perspective must be shifted from the present emphasis on cost-driven enhancement to revenue-driven improvements for growth. And since the product life-cycle is continuously shortening, competitiveness is linked very strongly with the capability to generate new technology products which enhance cost/benefit performance.     

Current status and future plans for the general antiparticle spectrometer (GAPS)

We discuss current progress and future plans for the general antiparticle spectrometer experiment (GAPS). GAPS detects antideuterons through the X-rays and pions emitted during the deexcitation of exotic atoms formed when the antideuterons are slowed down and stopped in targets. GAPS provides an exceptionally sensitive means to detect cosmic-ray antideuterons. Cosmic-ray antideuterons can provide indirect evidence for the existence of dark matter in such form as neutralinos or Kaluza–Klein particles. We describe results of accelerator testing of GAPS prototypes, tentative design concepts for a flight GAPS detector, and near-term plans for flying a GAPS prototype on a balloon.     

Studies of vegetation and soils from aerospace measurements

The problem of soil and vegetation recognition with the use of satellite-derived digitized images and sub-satellite spectral brightness measurements at test sites data is discussed. A technique for retrieval of soil humus content is suggested.     

The interest of information obtained by incoherent scatter technique

Incoherent scatter measurements are of great value for establishing and improving the IRI. This holds, in particular, for the topside electron density profile, for the valley occurring under certain conditions between regions E and F, and for the electron and ion temperature profiles. Extension in time of the observations could be very helpful for future work on IRI.     

Responses of ionospheric foF2 to geomagnetic activities in Hainan

Responses of low-latitude ionospheric critical frequency of F2 layer to geomagnetic activities in different seasons and under different levels of solar activity are investigated by analyzing the ionospheric foF2 data from DPS-4 Digisonde in Hainan Observatory during 2002–2005. The results are as follows: (1) the response of foF2 to geomagnetic activity in Hainan shows obvious diurnal variation except for the summer in low solar activity period. Generally, geomagnetic activity will cause foF2 to increase at daytime and decrease at nighttime. The intensity of response of foF2 is stronger at nighttime than that at daytime; (2) seasonal dependence of the response of foF2 to geomagnetic activity is very obvious. The negative ionospheric storm effect is the strongest in summer and the positive ionospheric storm effect is the strongest in winter; (3) the solar cycle has important effect on the response of foF2 to geomagnetic activity in Hainan. In high solar activity period, the diurnal variation of the response of foF2 is very pronounced in each season, and the strong ionospheric response can last several days. In low solar activity period, ionospheric response has very pronounced diurnal variation in winter only; (4) the local time of geomagnetic activities occurring also has important effect on the responses of foF2 in Hainan. Generally, geomagnetic activities occurred at nighttime can cause stronger and longer responses of foF2 in Hainan.     

Simulation experiments of the cosmic ray isotope telescope on ETS VI

A computer simulation was carried out to evaluate the basic characteristics of a Δ E×E cosmic ray telescope consisting of 23 solid state detectors including 3 position sensitive detectors with large effective area. Based on the simulation, the geometric factor of the telescope is deduced to be as large as 22.5 cm²sr, almost independent of charge and energy concerned. The energy ranges to be covered by the telescope are, for example, 18–98 MeV/n for Li and 56–339 MeV/n for Fe. By analyzing simulated data, the mass resolution for iron in the overall energy range covered by the telescope is estimated as about 0.22 amu in standard deviation. The expected counting rates and mass-histograms are simulated for Galactic cosmic rays and solar energetic particles.     

Phase separated membrane bioreactor: results from model system studies.

The operation and evaluation of a bioreactor designed for high intensity oxygen transfer in a microgravity environment is described. The reactor itself consists of a zero headspace liquid phase separated from the air supply by a long length of silicone rubber tubing through which the oxygen diffuses in and the carbon dioxide diffuses out. Mass transfer studies show that the oxygen is film diffusion controlled both externally and internally to the tubing and not by diffusion across the tube walls. Methods of upgrading the design to eliminate these resistances are proposed. Cell growth was obtained in the fermenter using Saccharomyces cerevisiae showing that this concept is capable of sustaining cell growth in the terrestrial [correction of terrestial] simulation.     

Rio de la Plata estuarine system: Relationship between river flow and frontal variability

This paper deals with the application of SeaWIFS images to characterize spatial and temporal variability of fronts in the Rio de la Plata estuarine system over the period 2000–2003. We aim to depict the relationship between river outflow and variability of fronts’ loci on monthly to ENSO-related timescales and the influence of the winds along Rio de la Plata (axial winds) on the abrupt changes in frontal dynamics over synoptic timescales. During the studied period both La Niña (July 1999–June 2000) and El Niño (April 2002–May 2003) events induced significant displacements of fronts. Three distinct fronts were analyzed between river, estuarine, coastal and marine waters of the Rio de la Plata: Main Turbidity Front, Main Marine Front, and Secondary Marine Front. Their number, location and separation seem to be mainly related to river outflow and second, to fresh (>8 m/s) axial winds. During low discharge periods (i.e. summer time and/or La Niña events) these winds induce abrupt changes in the location of fronts (100–200 km) and greater separation between them over synoptic timescale, whereas during high river discharge or ENSO years some of the variability of fronts location is explained by the river’s outflow fluctuations, especially by the high variability of the River Uruguay discharge.