The effects of microgravity on induced mutation in Escherichia coli and Saccharomyces cerevisiae.

We examined whether microgravity influences the induced-mutation frequencies through in vivo experiments during space flight aboard the space shuttle Discovery (STS-91). We prepared dried samples of repair-deficient strains and parental strains of Escherichia (E.) coli and Saccharomyces (S.) cerevisiae given DNA damage treatment. After culture in space, we measured the induced-mutation frequencies and SOS-responses under microgravity. The experimental findings indicate that almost the same induced-mutation frequencies and SOS-responses of space samples were observed in both strains compared with the ground control samples. It is suggested that microgravity might not influence induced-mutation frequencies and SOS-responses at the stages of DNA replication and/or DNA repair. In addition, we developed a new experimental apparatus for space experiments to culture and freeze stocks of E. coli and S. cerevisiae cells.     

A Far Infrared Photometer (FIRP) for the Infrared Telescope in Space (IRTS)

We describe the design and calibration of the Far-Infrared Photometer (FIRP), one of four focal plane instruments on the Infrared Telescope in Space (IRTS). The FIRP will provide absolute photometry in four bands centered at 150, 250, 400, and 700 μm with spectral resolution λ/Δλ ≈ 3 and spatial resolution ΔΘ = 0.5 degrees. High sensitivity is achieved by using bolometric detectors operated at 300 mK in an AC bridge circuit. The closed-cycle ³He refrigerator can be recycled in orbit. A 2 K shutter provides a zero reference for each field of view. More than 10% of the sky will be surveyed during the ≈3 week mission lifetime with a sensitivity of <10⁻¹³ W·cm⁻²·sr⁻¹ per 0.5 degree pixel.     

LET variation measurements behind different absorber thicknesses on COSMOS-1129 satellite

LET variation with the absorber thickness was measured by plastic detectors exposed on COSMOS-1129 satellite.     

Spectra and time variability of black-hole binaries in the low/hard state

We propose a jet model for the low/hard state of galactic black-hole X-ray sources which explains the energy spectra from radio to X-rays and a number of timing properties in the X-ray domain such as the time lag spectra, the hardening of the power density spectra and the narrowing of the autocorrelation function with increasing photon energy. The model assumes that (i) there is a magnetic field along the axis of the jet, (ii) the electron density in the jet drops inversely proportional to distance, (iii) the jet is “hotter” near its center than at its periphery, and (iv) the electrons in the jet follow a power-law distribution function. We have performed Monte Carlo simulations of Compton upscattering of soft photons from the accretion disk and have found power-law high-energy spectra with photon-number index in the range 1.5–2 and cutoff at a few hundred keV, power-law time lags versus Fourier frequency with index 0.8, and an increase of the rms amplitude of variability and a narrowing of the autocorrelation function with increasing photon energy as they have been observed in Cygnus X-1. The spectrum at long wavelengths (radio, infrared, optical) is modeled to come from synchrotron radiation of the energetic electrons in the jet. We find flat to inverted radio spectra that extend from the radio up to about the optical band. For magnetic field strengths of the order 10⁵–10⁶ G at the base of the jet, the calculated spectra agree well in slope and flux with the observations.     

Noise in wireless systems from solar radio bursts

Solar radio bursts were first discovered as result of their interference in early defensive radar systems during the Second World War (1942). Such bursts can still affect radar systems, as well as new wireless technologies. We have investigated a forty-year record of solar radio burst data (1960–1999) as well as several individual radio events in the 23rd solar cycle. This paper reviews the results of a portion of this research. Statistically, for frequencies f  1 GHz (near current wireless bands), there can be a burst with amplitudes >10³ solar flux units (SFU; 1 SFU = 10⁻²² W/m²) every few days during solar maximum conditions, and such burst levels can produce problems in contemporary wireless systems.     

Life sciences flight hardware development for the International Space Station.

During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners.     

Imaging the solar corona in the EUV

The SOHO (SOlar and Heliospheric Observatory) satellite was launched on December 2nd 1995. After arriving at the Earth-Sun (L1) Lagrangian point on February 14th 1996, it began to continuously observe the Sun. As one of the instruments onboard SOHO, the EIT (Extreme ultraviolet Imaging Telescope) images the Sun's corona in 4 EUV wavelengths. The He II filter at 304 Å images the chromosphere and the base of the transition region at a temperature of 5 − 8 × 10⁴ K; the Fe IX–X filter at 171 Å images the corona at a temperature of 1.3 × 10⁶ K; the Fe XII filter at 195 Å images the quiet corona outside coronal holes at a temperature of 1.6 × 10⁶ K; and the Fe XV filter at 284 Å images active regions with a temperature of 2.0 × 10⁶ K. About 5000 images have been obtained up to the present. In this paper, we describe also some aspects of the telescope and the detector performance for application in the observations. Images and movies of all the wavelengths allow a look at different phenomena present in the Sun's corona, and in particular, magnetic field reconnection.     

UV photobiochemistry under space conditions

The response of spores of Bacillus subtilis, cells of Deinococcus radiodurans and conidia of Aspergillus ochraceus to actual and simulated space conditions (UV in combination with long-term exposure to extremely dry conditions, including vacuum) has been studied: The following effects have been analyzed: decrease of viability, occurrence of DNA double strand breaks, formation of DNA-protein cross-links and DNA-DNA cross-links. All organisms show an increased sensitivity to UV light in extreme dryness (dry argon or vacuum) compared to an irradiation in aqueous suspension. The UV irradiation leads in all cases to a variety of DNA lesions. Very conspicuous is the occurrence of double strand breaks. Most of these double strand breaks are produced by incomplete repair of other lesions, especially base damages. The increase in DNA lesions can be correlated to the loss in viability. The specific response of the chromosomal DNA to UV irradiation in extreme dryness, however, varies from species to species and depends on the state of dehydration. The formation of DNA double strand breaks and DNA-protein cross-links prevails in the case of B. subtilis spores. In cells of Deinococcus radiodurans DNA-DNA cross-links often predominate, in conidia of Aspergillus ochraceus double strand breaks. The results obtained by direct exposure to space conditions (EURECA mission and D2 mission) largely agree with the laboratory data.     

Dynamic considerations for control of closed life support systems

Reliability of closed life support systems will depend on their ability to continue supplying the crew's needs in the face of perturbations and equipment failures. These dynamic considerations interact with the basic static (equilibrium) design through the sizing of storages, the specification of excess capacities in processors, and the choice of system initial state (total mass in the system). This paper uses a very simple system flow model to examine the possibilities for system failures even when there is sufficient storage to buffer the immediate effects of the perturbation. Two control schemes are shown which have different dynamic consequences in response to component failures.