Genomic instability and tumorigenic induction in immortalized human bronchial epithelial cells by heavy ions

Carcinogenesis is postulated to be a progressive multistage process characterized by an increase in genomic instability and clonal selection with each mutational event endowing a selective growth advantage. Genomic instability as manifested by the amplification of specific gene fragments is common among tumor and transformed cells. In the present study, immortalized human bronchial (BEP2D) cells were irradiated with graded doses of either 1GeV/nucleon ⁵⁶Fe ions or 150 keV/μm alpha particles. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Tumorigenic cells showed neither ras mutations nor deletion in the p16 tumor suppressor gene. In contrast, they harbored mutations in the p53 gene and over-expressed cyclin D1. Genomic instability among transformed cells at various stage of the carcinogenic process was examined based on frequencies of PALA resistance. Incidence of genomic instability was highest among established tumor cell lines relative to transformed, non-tumorigenic and control cell lines. Treatment of BEP2D cells with a 4 mM dose of the aminothiol WR-1065 significantly reduced their neoplastic transforming response to ⁵⁶Fe particles. This model provides an opportunity to study the cellular and molecular mechanisms involved in malignant transformation of human epithelial cells by heavy ions.     

Influence of the 27-day solar flux variations on the ionosphere parameters measured at Irkutsk in 2003–2005

We present an investigation of the influence of the 27-day solar flux variations, caused by solar rotation, on the ionosphere parameters such as the F2 layer critical frequency (foF2) and the total electron content (TEC). Our observational data were obtained with the Irkutsk Digisonde (DPS-4) located at 52.3 North and 104.3 East during the period from 2003 to 2005. In addition, we use TEC data from the Global Ionosphere Maps (GIM) based on Global Positioning System (GPS) satellites. The solar radiation flux at a wavelength of 10.7 cm (F10.7 index) is used as an index characterizing the solar activity level. A good correlation between observed ionosphere parameters and solar activity variations is found especially in autumn-to-winter season. We estimate the impact of the 27-day solar flux variations on the day-to-day variability and determine the time delay of the ionosphere response.     

Time-dependent thermal X-ray afterglows from GRBS

Time-dependent thermal X-ray spectra are calculated from physically plausible conditions around GRB. It is shown that account for time-dependent ionization processes strongly affects the observed spectra of hot rarefied plasma. These calculations may provide an alternative explanation to the observed X-ray lines of early GRBs afterglows (such as GRB 011211). Our technique will allow one to obtain independent constraints on the GRB collimation angle and on the clumpiness of circumstellar matter.     

UV photobiochemistry under space conditions

The response of spores of Bacillus subtilis, cells of Deinococcus radiodurans and conidia of Aspergillus ochraceus to actual and simulated space conditions (UV in combination with long-term exposure to extremely dry conditions, including vacuum) has been studied: The following effects have been analyzed: decrease of viability, occurrence of DNA double strand breaks, formation of DNA-protein cross-links and DNA-DNA cross-links. All organisms show an increased sensitivity to UV light in extreme dryness (dry argon or vacuum) compared to an irradiation in aqueous suspension. The UV irradiation leads in all cases to a variety of DNA lesions. Very conspicuous is the occurrence of double strand breaks. Most of these double strand breaks are produced by incomplete repair of other lesions, especially base damages. The increase in DNA lesions can be correlated to the loss in viability. The specific response of the chromosomal DNA to UV irradiation in extreme dryness, however, varies from species to species and depends on the state of dehydration. The formation of DNA double strand breaks and DNA-protein cross-links prevails in the case of B. subtilis spores. In cells of Deinococcus radiodurans DNA-DNA cross-links often predominate, in conidia of Aspergillus ochraceus double strand breaks. The results obtained by direct exposure to space conditions (EURECA mission and D2 mission) largely agree with the laboratory data.     

A Unified Approach to Model-Reference Adaptive Systems Part I: Theory

A unified approach to model-reference adaptive control systems is presented. A linearized error equation for each adaptive system is derived about some operating point and converted into the standard form for plotting root loci. The contribution made here is to convert the selection of an adaptive technique and the choosing of adaptive coefficients for some desired system performance from a "mystical art" to standard control techniquess.     

Simple adaptive control for aircraft control surface failures

This work extends the so-called simple adaptive control approach to direct model reference adaptive control of multi-input multi-output systems to include loss of control effectiveness failures. It is proven that all signals are bounded for loss of control effectiveness failures during a bounded input disturbance. A state space approach is introduced for computing the feedforward compensator that is required by the stability result. The adaptive algorithm is applied to a three input model of the linearized lateral dynamics of the F/A-18 aircraft. Simulation results are obtained with single, double, and triple control effectiveness failures of 88% during the occurrence of a lateral gust. These results show that the adaptive controller exhibits improved model following as compared with a fixed gain eigenstructure assignment controller.     

Almost instant time inference for hybrid partially dynamic Bayesian networks

A Bayesian network (BN) is a compact representation for probabilistic models and inference. They have been used successfully for many military and civilian applications. It is well known that, in general, the inference algorithms to compute the exact a posterior probability of a target node given observed evidence are either computationally infeasible for dense networks or impossible for general hybrid networks. In those cases, one either computes the approximate results using stochastic simulation methods or approximates the model using discretization or a Gaussian mixture model before applying an exact inference algorithm. This paper combines the concept of simulation and model approximation to propose an efficient algorithm for those cases. The main contribution here is a unified treatment of arbitrary (nonlinear non-Gaussian) hybrid (discrete and continuous) BN inference having both computation and accuracy scalability. The key idea is to precompile the high-dimensional hybrid distribution using a hypercube representation and apply it for both static and dynamic BN inference. Since the inference process essentially becomes a combination of table look-up and some simple operations, the method is shown to be extremely efficient. It can also he scaled to achieve any desirable accuracy given sufficient preprocessing time and memory for the cases where exact inference is not possible     

On the dynamics of a novel manipulator with slewing and deployable links

Using a novel space platform-based manipulator with slewing and deployable links, the paper addresses two issues of considerable importance: (a) How important is it to model flexibility of the system? (b) How many modes are needed to adequately represent the elastic character? Results suggest that the fundamental mode is able to capture physics of the response quite accurately. Due to its massive character, the platform dynamics is virtually unaffected, even by severe maneuvers of the manipulator. Hence, treating the platform as rigid would save the computational cost without affecting the accuracy. Although the link flexibility does affect the manipulator's tip vibration, the joint and platform vibrations remain negligible. The revolute joint flexibility appears to be an important parameter affecting both the joint as well as tip responses. The information should prove useful in the design of this new class of manipulators.