Pleurodeles waltl, amphibian, Urodele, is a suitable biological model for embryological and physiological space experiments on a vertebrate.

Pleurodeles waltl (amphibian, Urodele) is an appropriate biological model for space experiments on a vertebrate. One reason for interest in this animal concerns the study of the effects of absence of gravity on embryonic development. First, after mating (on Earth) the females retain live, functional sperm in their cloacum for up to 5 months, allowing normal in vivo fertilisation after hormonal stimulation. Second, their development is slow, which allows analyses of all the key stages of ontogenesis from the oocyte to swimming tailbud embryos or larvae. We have performed detailed studies and analyses of the effects of weightlessness on amphibian Pleurodeles embryos, fertilised and allowed to develop until the swimming larvae stage. These experiments were performed in space during three missions on the MIR-station: FERTILE I, FERTILE II and NEUROGENESIS respectively in 1996, 1998 and 1999. We show that in microgravity abnormalities appeared at specific stages of development compared to 1g-centrifuge control embryos and 1g-ground control embryos. In this report we describe abnormalities occurring in the central nervous system. These modifications occur during the neurulation process (delay in the closure of the neural tube and failure of closure of this tube in the cephalic area) and at the early tailbud stage (microcephaly observed in 40% of the microgravity-embryos). However, if acephalic and microcephalic embryos are not taken into account, these abnormalities did not disturb further morphological, biochemical and functional development and the embryos were able to regulate and a majority of normal hatching and swimming larvae were obtained in weightlessness with a developmental time-course equivalent to that of 1g-centrifuge control embryos (on the MIR station) and 1g-ground control embryos.     

Current barriers and factors of success in the diffusion of satellite services in Europe

Satellite services benefit civil society by helping tackle challenges such as climate change, the digital divide, etc. They have the potential to deliver concrete benefits to European society through innovative services supporting economic, societal and environmental policies. Such benefits can trigger increased public support for space in Europe. However, this potential has yet to be achieved. This paper argues that technological bias, the diversity of interests and initiatives among stakeholders and their individual actions do not always serve their collective objective to ensure wide diffusion of satellite services. It draws on theories of diffusion of innovation and on its authors' participatory work with the space and the user communities and at their interface in an effort to help diffuse satellite services within civil society. One of the major causes of insufficient service diffusion is the weakness of the interface between the space and user communities; some of factors that currently contribute to this state of affairs are the space community's over-reliance on publicly financed, technical demonstration projects as solutions to service diffusion; insufficient coordination by public authorities of innovation policies and programmes with other public policies and objectives; and an insufficient integration of satellite services within users' culture, traditional tools and services. The discussion allows for conclusions to be drawn on how the system of stakeholders could function better in order for satellite services to be successfully diffused in Europe.     

Resistance of bacterial endospores to outer space for planetary protection purposes--experiment PROTECT of the EXPOSE-E mission

Spore-forming bacteria are of particular concern in the context of planetary protection because their tough endospores may withstand certain sterilization procedures as well as the harsh environments of outer space or planetary surfaces. To test their hardiness on a hypothetical mission to Mars, spores of Bacillus subtilis 168 and Bacillus pumilus SAFR-032 were exposed for 1.5 years to selected parameters of space in the experiment PROTECT during the EXPOSE-E mission on board the International Space Station. Mounted as dry layers on spacecraft-qualified aluminum coupons, the "trip to Mars" spores experienced space vacuum, cosmic and extraterrestrial solar radiation, and temperature fluctuations, whereas the "stay on Mars" spores were subjected to a simulated martian environment that included atmospheric pressure and composition, and UV and cosmic radiation. The survival of spores from both assays was determined after retrieval. It was clearly shown that solar extraterrestrial UV radiation (λ≥110?nm) as well as the martian UV spectrum (λ≥200?nm) was the most deleterious factor applied; in some samples only a few survivors were recovered from spores exposed in monolayers. Spores in multilayers survived better by several orders of magnitude. All other environmental parameters encountered by the "trip to Mars" or "stay on Mars" spores did little harm to the spores, which showed about 50% survival or more. The data demonstrate the high chance of survival of spores on a Mars mission, if protected against solar irradiation. These results will have implications for planetary protection considerations.     

In vivo fertilization and development in microgravity using pleurodele ("ZEUS" project).

The objectives of this experiment are to perform natural fertilization and to achieve embryonic development in microgravity. Pleurodeles waltl, an urodele amphibian, is considered by CNES and NASA to be suitable experimental material for achieving in vivo fertilization in space. Previously inseminated females can be embarked in the Frog Environmental Unit (FEU) developed by NASA. Laying of eggs will be provoked by hormonal stimulation in flight and development will be followed. Various technical problems have been resolved in laboratory experiments and during parabolic flights : the time of hormone stimulation after insemination, choice of hormone guaranteeing [correction of guarenteing] 95% success, other factors conditioning [correction of conditionning] the laying, experimental procedures to study developmental kinetics at phenotypic levels, and selection of cellular and molecular markers of development.     

A high-order SPH method by introducing inverse kernels

The smoothed particle hydrodynamics (SPH) method is usually expected to be an effi-cient numerical tool for calculating the fluid-structure interactions in compressors; however, an endogenetic restriction is the problem of low-order consistency. A high-order SPH method by intro-ducing inverse kernels, which is quite easy to be implemented but efficient, is proposed for solving this restriction. The basic inverse method and the special treatment near boundary are introduced with also the discussion of the combination of the Least-Square (LS) and Moving-Least-Square (MLS) methods. Then detailed analysis in spectral space is presented for people to better under-stand this method. Finally we show three test examples to verify the method behavior.     

Detection of Envisat RA2/ICE-1 retracked radar altimetry bias over the Amazon basin rivers using GPS

Altimetry is now routinely used to monitor stage variations over rivers, including in the Amazon basin. It is desirable for hydrologic studies to be able to combine altimetry from different satellite missions with other hydrogeodesy datasets such as leveled gauges and watershed topography. One requirement is to accurately determine altimetry bias, which could be different for river studies from the altimetry calibrated for deep ocean or lake applications. In this study, we estimate the bias in the Envisat ranges derived from the ICE-1 waveform retracking, which are nowadays widely used in hydrologic applications. As a reference, we use an extensive dataset of altitudes of gauge zeros measured by GPS collocated at the gauges. The thirty-nine gauges are spread along the major tributaries of the Amazon basin. The methodology consists in jointly modeling the vertical bias and spatial and temporal slope variations between altimetry series located upstream and downstream of each gauge. The resulting bias of the Envisat ICE-1 retracked altimetry over rivers is 1.044 ± 0.212 m, revealing a significant departure from other Envisat calibrations or from the Jason-2 ICE-1 calibration.