Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems.

Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. Grant numbers: 99-04-96017, 00-07-9011.     

Molecular analysis of mutagenesis by high LET radiation.

Mutation induction by high linear energy transfer [LET] alpha particles and gamma-rays was scored in the human hamster hybrid [AL] cells. Southern blotting technique was used to analyse the molecular changes in the DNA from both the HGPRT- and S1- mutants. Dose dependent mutagenesis in the AL cells irradiated with the charged particles was higher by almost 20 fold at the S1 than the corresponding HGPRT locus. Southern analysis of the mutants induced by the high LET particles showed mostly multilocus deletion at both the HGPRT and S1 genes.     

Surveillance through walls and other opaque materials

The Department of Defense (DoD) has funded a dazzling array of “high tech” solutions for many of the problems facing our military forces. Many of these “solutions” have been effective for long range mass destruction but have not been applicable for the close-in hand-to-hand combat that is on our streets. Our goal at the Hughes AET Center has been to convert “high tech” DoD capabilities into cost effective tools to help law enforcement agencies do their jobs better. Surveillance systems presently used by law enforcement officers make extensive use of television, infrared and other Line-of-Sight (LOS) surveillance systems. However, these systems cannot tell what is happening on the other side of a wall, behind bushes, around the corner, in the dark or through a dense fog. A new sensor has been developed that uses technology developed by the DoD for missile warhead fuzing. This small, light weight, low power “Radar” is based upon the fact that radio waves can penetrate nonmetallic materials. This new surveillance capability can help provide information about what is in a wall, ceiling or floor or on the other side of a door or concrete wall. Real field scenarios are used in this paper to show how this radar works and how field users can tell if someone is moving inside a building, even from remote locations     

Giotto/Cometary dust interaction event near the comet Halley ionopause

Plasma and magnetic field disturbances accompanying dust particle impacts are explained by means of creation of a secondary cloud around the spacecraft. Cold cometary ions impinging upon the cloud are scattered by atoms of the cloud. This scattering changes initial angular distribution of cometary ions. Magnetic field perturbation is created by the friction between the electron component of the cometary plasma flow and the cloud.     

Carbon dioxide exchange of lettuce plants under hypobaric conditions.

Growth of plants in a Controlled Ecological Life Support System (CELSS) may involve the use of hypobaric pressures enabling lower mass requirements for atmospheres and possible enhancement of crop productivity. A controlled environment plant growth chamber with hypobaric capability designed and built at Ames Research Center was used to determine if reduced pressures influence the rates of photosynthesis (Ps) and dark respiration (DR) of hydroponically grown lettuce plants. The chamber, referred to as a plant volatiles chamber (PVC), has a growing area of about 0.2 m2, a total gas volume of about 0.7 m3, and a leak rate at 50 kPa of <0.1%/day. When the pressure in the chamber was reduced from ambient to 51 kPa, the rate of net Ps increased by 25% and the rate of DR decreased by 40%. The rate of Ps increased linearly with decreasing pressure. There was a greater effect of reduced pressure at 41 Pa CO2 than at 81 Pa CO2. This is consistent with reports showing greater inhibition of photorespiration (Pr) in reduced O2 at low CO2 concentrations. When the partial pressure of O2 was held constant but the total pressure was varied between 51 and 101 kPa, the rate of CO2 uptake was nearly constant, suggesting that low pressure enhancement of Ps may be mainly attributable to lowered partial pressure of O2 and the accompanying reduction in Pr. The effects of lowered partial pressure of O2 on Ps and DR could result in substantial increases in the rates of biomass production, enabling rapid throughput of crops or allowing flexibility in the use of mass and energy resources for a CELSS.     

DNA damage and repair in oncogenic transformation by heavy ion radiation.

Energetic heavy ions are present in galactic cosmic rays and solar particle events. One of the most important late effects in risk assessment is carcinogenesis. We have studied the carcinogenic effects of heavy ions at the cellular and molecular levels and have obtained quantitative data on dose-response curves and on the repair of oncogenic lesions for heavy particles with various charges and energies. Studies with repair inhibitors and restriction endonucleases indicated that for oncogenic transformation DNA is the primary target. Results from heavy ion experiments showed that the cross section increased with LET and reached a maximum value of about 0.02 micrometer2 at about 500 keV/micrometer. This limited size of cross section suggests that only a fraction of cellular genomic DNA is important in radiogenic transformation. Free radical scavengers, such as DMSO, do not give any effect on induction of oncogenic transformation by 600 MeV/u iron particles, suggesting most oncogenic damage induced by high-LET heavy ions is through direct action. Repair studies with stationary phase cells showed that the amount of reparable oncogenic lesions decreased with an increase of LET and that heavy ions with LET greater than 200 keV/micrometer produced only irreparable oncogenic damage. An enhancement effect for oncogenic transformation was observed in cells irradiated by low-dose-rate argon ions (400 MeV/u; 120 keV/micrometer). Chromosomal aberrations, such as translocation and deletion, but not sister chromatid exchange, are essential for heavy-ion-induced oncogenic transformation. The basic mechanism(s) of misrepair of DNA damage, which form oncogenic lesions, is unknown.     

The status of qualification of a new separator system for advancedaerospace battery cells

A nickel cadmium cell system which utilizes a polypropylene separator impregnated with polybenzimadazole, and which shows promise of providing an aerospace battery with performance equivalent to Super NiCd, and yet is more cost effective, is described. Background information, cell construction information, detailed test program information and data, and status of qualification are given     

Optimal radar threshold determination in Weibull clutter andGaussian noise

A technique is presented for determining the ideal detection threshold when Gaussian noise and Weibull distributed clutter returns are present on a radar receiver and neither is dominant. Quantitative data is presented for several clutter types and false alarm probabilities     

Low energy ions in the heavy ions in space (HIIS) experiment on LDEF.

We present data from the Lexan top stacks in the Heavy Ions In Space (HIIS) experiment which was flown for six years (April 1984-Jan 1990) onboard the LDEF spacecraft in 28.5 degrees orbit at about 476 km altitude. HIIS was built of passive (i.e. no timing resolution) plastic track detectors which collected particles continuously over the entire mission. In this paper we present data on low energy heavy ions (10 < or = Z, 20MeV/nuc < E < 200 MeV/nuc). These ions are far below the geomagnetic cutoff for fully ionized ions in the LDEF orbit even after taking into account the severe cutoff suppression caused by occasional large geomagnetic storms during the LDEF mission. Our preliminary results indicate an unusual elemental composition of trapped particles in the inner magnetosphere during the LDEF mission, including both trapped anomalous cosmic ray species (Ne, Ar) and other elements (such as Mg and Fe) which are not found in the anomalous component of cosmic rays. The origin of the non-anomalous species is not understood, but they may be associated with the solar energetic particle events and geomagnetic disturbances of 1989.     

Systems integration, user interface design, and tower air trafficcontrol

Tower air traffic control is currently performed using several unintegrated systems. While each of these systems supports performing an isolated task, the compilation of tools presents a number of challenges. This paper reports ways in which the Tower Control Computer Complex (TCCC) will support and enhance user management of numerous systems. Areas that will be notably improved include the consistency in the user interface of the air traffic control toolset, the task of manually integrating tools and information, toolset alert management, and toolset organization and administration