Entrapment of bacteria in fluid inclusions in laboratory-grown halite

Cells of the bacterium Pseudomonas aeruginosa, which were genetically modified to produce green fluorescent protein, were entrapped in fluid inclusions in laboratory-grown halite. The bacteria were used to inoculate NaCl-saturated aqueous solutions, which were allowed to evaporate and precipitate halite. The number, size, and distribution of fluid inclusions were highly variable, but did not appear to be affected by the presence of the bacteria. Many of the inclusions in crystals from inoculated solutions contained cells in populations ranging from two to 20. Microbial attachment to crystal surfaces was neither evident nor necessary for entrapment. Cells occurred exclusively within fluid inclusions and were not present in the crystal matrix. In both the inclusions and the hypersaline solution, the cells fluoresced and twitched, which indicates that the bacteria might have remained viable after entrapment. The fluorescence continued up to 13 months after entrapment, which indicates that little degradation of the bacteria occurred over that time interval. The entrapment, fluorescence, and preservation of cells were independent of the volume of hypersaline solution used or whether the solutions were completely evaporated prior to crystal extraction. The results of this study have a wide range of implications for the long-term survival of microorganisms in fluid inclusions and their detection through petrography. The results also demonstrate the preservation potential for microbes in hypersaline fluid inclusions, which could allow cells to survive harsh conditions of space, the deep geologic past, or burial in sedimentary basins.     

Evolution of executive branch space policy making

This paper assesses the impacts that changes in the US presidency have had on the civilian space program during the post-Apollo period. It suggests that the White House has lost influence over space issues relative to other interested sectors. The primary factors causing this are a long-term shift to lower priority for these issues, and an inevitable widening of the scope of influence over this sector of activity. Key post-Apollo decisions are discussed for their substantive content and also for what they reveal about the extent to which the president chose to be involved. These findings have implications across all science and technology issue areas as the USA, in the aftermath of the Cold War, reexamines its post-World War II social contract with science.     

Low altitude wind shear detection using airport surveillance radars

This paper describes an enhanced weather processor for the Federal Aviation Administration's Airport Surveillance Radar (ASR-9) that will include Doppler wind estimation for the detection of low altitude wind shear, scan-to-scan tracking to provide estimates of the speed and direction of storm movement and suppression' of spurious weather reports currently generated by the ASR-9's six-level weather channel during episodes of anomalous radar energy propagation (AP). This ASR-9 Wind Shear Processor (WSP) will be implemented as a retrofit to the ASR-9 through the addition of interfaces, receiving chain hardware and high-speed digital processing and display equipment. Thunderstorm activity in terminal airspace (the volume extending approximately 30 nmi from an airport and to 15,000 feet altitude) is an obvious safety issue and makes a significant overall contribution to delay in the United States commercial aviation industry. Analysis and on-line testing of the prototype ASR-9 WSP has confirmed that the system can provide operationally beneficial detection of low-altitude wind shear phenomena and enhanced weather situational awareness for Air Traffic Control teams     

Observations of solar radio bursts from meter to kilometer wavelengths

Using the Clark Lake Radioheliograph data we present direct evidence that type III electron streams propagate in dense coronal streamers. We also present imaging observations of meter-decameter microbursts, which appear to be similar to those observed in hard X-rays. At meter-decameter wavelengths, these microbursts appear to be due to plasma radiation. From observations made with ISSE-3, we discuss the characteristics of hectometer and kilometer wavelength radio bursts. In particular, we show that from studies of type III storms that the exciter electrons propagate along spiral structures, where the density is enhanced and that there is an acceleration of the solar wind. We discuss type II bursts at kilometer wavelengths, compare them with meter type II bursts and discuss their association with interplanetary shocks. We show that the interaction between type III electron streams and shocks at kilometer wavelengths can provide information on the interplanetary shock geometry. Finally, we discuss the possibility that some shock associated (SA) events may be emissions caused by electrons accelerated lower in the atmosphere rather than high in the corona in type II shocks.Recent advances in solar research have resulted from new work on plasma radiation theory, new observations of active regions and flares across the electromagnetic spectrum and the availability of spacecraft in situ measurements of solar ejecta. In this paper, we review some results obtained with the Clark Lake multifrequency radioheliograph at meter-decameter wavelengths and from satellite multifrequency directive observations at hectometer and kilometer wavelengths. We present evidence that type III electrons propagate in dense coronal streamers, and that frequently observed microbursts (presumably of type III) at meter-decameter wavelengths are due to plasma radiation. We discuss observations of hectometer and kilometer type III radio storms which reveal information about active region structures, interplanetary magnetic field configuration, and solar wind acceleration. We also discuss kilometer type II bursts, interactions between type III electrons and interplanetary shocks, and present some new results on shock associated (SA) events.     

Lick Observatory Optical SETI: targeted search and new directions

Lick Observatory's Optical SETI (search for extraterrestrial intelligence) program has been in regular operation for 4.5 years. We have observed 4,605 stars of spectral types F-M within 200 light-years of Earth. Occasionally, we have appended objects of special interest, such as stars with known planetary systems. We have observed 14 candidate signals ("triple coincidences"), all but one of which are explained by transient local difficulties. Additional observations of the remaining candidate have failed to confirm arriving pulse events. We now plan to proceed in a more economical manner by operating in an unattended drift scan mode. Between operational and equipment modifications, efficiency will more than double.     

Electronics brain design for advanced microspacecraft

A brief history of NASA robotic spacecraft computing architectures is given. Changing needs driving changes in features (10x reduction in cost, various increases in capability, 10x to 100x reduction in power, mass and volume) for spacecraft computing are discussed. An architecture which addresses these changes is proposed. Finally, future directions of exploration for this spacecraft computing architecture are considered.     

The real aerospace plane

In this article reproduced from Aerospace, Spring 1986, James Haggerty reports on the US National Aerospace Plane (NASP) research programme. NASP is a joint Department of Defense/NASA programme to develop and demonstrate the technologies for a revolutionary class of aerospace vehicles, powered by airbreathing engines, that would have the capability to take off from and land horizontally on standard runways, cruise in the atmosphere at hypersonic speeds, or fly into low-Earth orbit. The Air Force has been named executive agency for the programme. NASA is charged with overall technology direction. Other DoD participants include DARPA, the Navy and the Strategic Defense Initiative Organization.

In April 1986, the USAF awarded development contracts to seven aerospace manufacturing firms. Contracts for air-frame design went to Boeing Aerospace Company, Lockheed Advanced Aeronautics Company, General Dynamics Corporation, McDonnell Douglas Corporation and Rockwell International Corporation. General Electric Company and Pratt & Whitney Division of United Technologies were awarded propulsion contracts.     

Extremophiles may be irrelevant to the origin of life

In recent years, Bacteria and Archaea have been discovered living in practically every conceivable terrestrial environment, including some previously thought to be too extreme for survival. Exploration of our solar system has revealed a number of extraterrestrial bodies that harbor environments analogous to many of the terrestrial environments in which extremophiles flourish. The recent discovery of more than 105 extrasolar planets suggests that planetary systems are quite common. These three findings have led some to speculate that life is therefore common in the universe, as life as we know it can seemingly survive almost anywhere there is liquid water. It is suggested here that while environments capable of supporting life may be common, this does not in itself support the notion that life is common in the universe. Given that interplanetary transfer of life may be unlikely, the actual origin of life may require specific environmental and geological conditions that may be much less common than the mere existence of liquid water.     

Solar Isotopic Composition as Determined Using Solar Energetic Particles

Solar energetic particles (SEPs) provide a sample of the Sun from which solar composition may be determined. Using high-resolution measurements from the Solar Isotope Spectrometer (SIS) onboard NASA’s Advanced Composition Explorer (ACE) spacecraft, we have studied the isotopic composition of SEPs at energies ≥20 MeV/nucleon in large SEP events. We present SEP isotope measurements of C, O, Ne, Mg, Si, S, Ar, Ca, Fe, and Ni made in 49 large events from late 1997 to the present. The isotopic composition is highly variable from one SEP event to another due to variations in seed particle composition or due to mass fractionation that occurs during the acceleration and/or transport of these particles. We show that various isotopic and elemental enhancements are correlated with each other, discuss the empirical corrections used to account for the compositional variability, and obtain estimated solar isotopic abundances. We compare the solar values and their uncertainties inferred from SEPs with solar wind and other solar system abundances and find generally good agreement.