UV photobiochemistry under space conditions

The response of spores of Bacillus subtilis, cells of Deinococcus radiodurans and conidia of Aspergillus ochraceus to actual and simulated space conditions (UV in combination with long-term exposure to extremely dry conditions, including vacuum) has been studied: The following effects have been analyzed: decrease of viability, occurrence of DNA double strand breaks, formation of DNA-protein cross-links and DNA-DNA cross-links. All organisms show an increased sensitivity to UV light in extreme dryness (dry argon or vacuum) compared to an irradiation in aqueous suspension. The UV irradiation leads in all cases to a variety of DNA lesions. Very conspicuous is the occurrence of double strand breaks. Most of these double strand breaks are produced by incomplete repair of other lesions, especially base damages. The increase in DNA lesions can be correlated to the loss in viability. The specific response of the chromosomal DNA to UV irradiation in extreme dryness, however, varies from species to species and depends on the state of dehydration. The formation of DNA double strand breaks and DNA-protein cross-links prevails in the case of B. subtilis spores. In cells of Deinococcus radiodurans DNA-DNA cross-links often predominate, in conidia of Aspergillus ochraceus double strand breaks. The results obtained by direct exposure to space conditions (EURECA mission and D2 mission) largely agree with the laboratory data.     

HZE-dosimetry on MIR92, IML-1 and D-2

Nuclear track detectors were used to measure the integral Linear Energy Transfer (LET) spectra above 1 GeV per cm water behind the complex material shielding inside a spacecraft. The measurements are compared with predictions of the contribution of high charge, high energy HZE particles of the galactic cosmic radiation taking into account the influence of solar and geomagnetic modulation and shielding by matter.     

Use of combined light flash and plasma measurements to study hypervelocity impact processes

We present new measurements concerning generation of light flash during hypervelocity impacts. We use iron particles (10⁻¹³ to 10⁻¹⁷ kg) with velocities over the range 1 to 42 km/s impacting semi-infinite targets (aluminium and molybdenum). The main results of previous work in the field are found to be reproduced with some slight deviations. For iron projectiles with given mass and velocity the energy of the flash (normalized to mass) is proportional to velocity to the power of 3.5 for aluminium targets and 3.9 for molybdenum targets. The duration of the flash is of order 1 microsecond. Simultaneous measurements of the generation of impact plasma do not change this. The onset of plasma generation of the bulk target material does not affect the total light flash energy. We discuss the duration of the flash compared to a simple calculation of temperature in the target and plasma vs time.     

The Spacelab-Mir-1 "Greenhouse-2" experiment.

The Spacelab-Mir-1 (SLM-1) mission is the first docking of the Space Shuttle Atlantis (STS-71) with the Orbital Station Mir in June 1995. The SLM-1 "Greenhouse-2" experiment will utilize the Russian-Bulgarian-developed plant growth unit (Svet). "Greenhouse-2" will include two plantings (1) designed to test the capability of Svet to grow a crop of Superdwarf wheat from seed to seed, and (2) to provide green plant material for post-flight analysis. Protocols, procedures, and equipment for the experiment have been developed by the US-Russian science team. "Greenhouse-2" will also provide the first orbital test of a new Svet Instrumentation System (SIS) developed by Utah State University to provide near real time data on plant environmental parameters and gas-exchange rates. SIS supplements the Svet control and monitoring system with additional sensors for substrate moisture, air temperature, IR leaf temperature, light, oxygen, pressure, humidity, and carbon-dioxide. SIS provides the capability to monitor canopy transpiration and net assimilation of the plants growing in each vegetation unit (root zone) by enclosing the canopy in separate, retractable, ventilated leaf chambers. Six times during the seed-to-seed experiment, plant samples will be collected, leaf area measured, and plant parts fixed and/or dried for ground analysis. A second planting initiated 30 days before the arrival of a U.S. Shuttle [originally planned to be STS-71] is designed to provide green material at the vegetative development stage for ground analysis. [As this paper is being edited, the experiment has been delayed until after the arrival of STS-71.]     

Shock protection of penetrator-based instrumentation via a sublimation approach

It is often necessary for space-borne instrumentation to cope with substantial levels of shock acceleration both in the initial launch phase, as well as during entry, descent and landing in the case of planetary exploration. Current plans for a new generation of penetrator-based space missions will subject the associated on-board instrumentation to far greater levels of shock, and ways must therefore be found to either ruggedize or else protect any sensitive components during the impact phase. In this paper, we present an innovative method of shock protection that is suited for use in a number of planetary environments, based upon the temporary encapsulation of said components within a waxy solid which may then be sublimated to return the instrument back to its normal operation. We have tested this method experimentally using micromachined silicon suspensions under applied shock loads of up to 15,000g, and found that these were able to survive without incurring damage. Furthermore, quality factor measurements undertaken on these suspensions indicate that their mechanical performance remains unaffected by the encapsulation and subsequent sublimation process.     

Radio fine structures in dm–cm wavelength range associated with magnetic reconnection processes

Radio bursts with fine structures in decimetric–centimetric wave range are generally believed to manifest the primary energy release process during flare/CME events. By spectropolarimeters in 1–2 GHz, 2.6–3.8 GHz, and 5.2–7.6 GHz at NAOC/Huairou with very high temporal (1.25–8 ms) and spectral (4–20 MHz) resolutions, the zebra patterns, spikes, and new types of radio fine structures with mixed frequency drift features are observed during several significant flare/CME events. In this paper we will discuss the occurrence of radio fine structures during the impulsive phase of flares and/or CME initiations, which may be connected to the magnetic reconnection processes.     

Telemetry,telecommand and safety sub-systems for scientific ballooning from Hyderabad

Highly sophisticated balloon-borne scientific payloads have stringent requirement on the telemetry and command system. The development and fabrication of the on-board TT&C package for telemetry, tracking, command, safety and ranging for these experiments is done in-house at the National Balloon Facility (NBF) at Hyderabad. In the last few years, we have made major improvements both in the ground station and the on-board sub-systems, thereby improving the data quality, data handling speed and the general flight control along with aviation safety. The new system has telemetry data rate up to 1 Mbps. A reduction in weight, power and cost of the reengineered on-board integrated package has also lead to the ease of operation during field tests prior to launch and at remote recovery sites. In this paper, we describe the details of the new control package, its flight performance and our plans for portable S-band telemetry and telecommand system to cater to the balloon flights from Antarctic station and long duration balloon flights.     

Survival and death of the haloarchaeon Natronorubrum strain HG-1 in a simulated martian environment

Halophilic archaea are of interest to astrobiology due to their survival capabilities in desiccated and high salt environments. The detection of remnants of salty pools on Mars stimulated investigations into the response of haloarchaea to martian conditions. Natronorubrum sp. strain HG-1 is an extremely halophilic archaeon with unusual metabolic pathways, growing on acetate and stimulated by tetrathionate. We exposed Natronorubrum strain HG-1 to ultraviolet (UV) radiation, similar to levels currently prevalent on Mars. In addition, the effects of low temperature (4, −20, and −80 °C), desiccation, and exposure to a Mars soil analogue from the Atacama desert on the viability of Natronorubrum strain HG-1 cultures were investigated. The results show that Natronorubrum strain HG-1 cannot survive for more than several hours when exposed to UV radiation equivalent to that at the martian equator. Even when protected from UV radiation, viability is impaired by a combination of desiccation and low temperature. Desiccating Natronorubrum strain HG-1 cells when mixed with a Mars soil analogue impaired growth of the culture to below the detection limit. Overall, we conclude that Natronorubrum strain HG-1 cannot survive the environment currently present on Mars. Since other halophilic microorganisms were reported to survive simulated martian conditions, our results imply that survival capabilities are not necessarily shared between phylogenetically related species.     

The role of space related non-governmental organizations (NGOs) in capacity building

Non-governmental organizations (NGOs) play a unique role in international affairs, providing access to resources, expertise, and assistance to supplement State resources. Sometimes the diplomatic skills and unofficial access of NGOs to policymakers through Track Two diplomacy can move a previously stalled critical issue forward and assist policymakers from different countries to find common ground outside official channels. Because they work outside of official channels, they are not bound by State policy that may inhibit negotiations between States. Some also have a convening power that sometimes makes it possible for State representatives to meet discipline experts and each other for informal discussions on issues of mutual interest. Finally, NGOs can draw attention to issues that may be overlooked or avoided by State organizations.