Design of a Correlator for Real-Time Video Comparisons

The problem of locating a reference image within a larger image using a correlation technique is discussed. Although the particular application discussed is that of locating a reference image obtained from one video sensor or a photograph, within the larger field of view obtained from a second video sensor in real time (i.e., at the TV field rate), the results are general and useful for a number of applications. The tradeoffs necessary to obtain real time correlat are discussed and their effect on correlation accuracy is given.     

Early development of fern gametophytes in microgravity.

Dormant spores of the fern Ceratopteris richardii were flown on Shuttle mission STS-93 to evaluate the effects of micro-g on their development and on their pattern of gene expression. Prior to flight the spores were sterilized and sown into one of two environments: (1) Microscope slides in a video-microscopy module; and (2) Petri dishes. All spores were then stored in darkness until use. Spore germination was initiated on orbit after exposure to light. For the spores on microscope slides, cell level changes were recorded through the clear spore coat of the spores by video microscopy. After their exposure to light, spores in petri dishes were frozen in orbit at four different time points during which on earth gravity fixes the polarity of their development. Spores were then stored frozen in Biological Research in Canister units until recovery on earth. The RNAs from these cells and from 1-g control cells were extracted and analyzed on earth after flight to assay changes in gene expression. Video microscopy results revealed that the germinated spores developed normally in microgravity, although the polarity of their development, which is guided by gravity on earth, was random in space. Differential Display-PCR analyses of RNA extracted from space-flown cells showed that there was about a 5% change in the pattern of gene expression between cells developing in micro-g compared to those developing on earth.     

Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation.

The lens epithelium is the initiation site for the development of radiation induced cataracts. Radiation in the cortex and nucleus interacts with proteins, while in the epithelium, experimental results reveal mutagenic and cytotoxic effects. It is suggested that incorrectly repaired DNA damage may be lethal in terms of cellular reproduction and also may initiate the development of mutations or transformations in surviving cells. The occurrence of such genetically modified cells may lead to lens opacification. For a quantitative risk estimation for astronauts and space travelers it is necessary to know the relative biological effectiveness (RBE), because the spacial and temporal distribution of initial physical damage induced by cosmic radiation differ significantly from that of X-rays. RBEs for the induction of DNA strand breaks and the efficiency of repair of these breaks were measured in cultured diploid bovine lens epithelial cells exposed to different LET irradiation to either 300 kV X-rays or to heavy ions at the UNILAC accelerator at GSI. Accelerated ions from Z=8 (O) to Z=92 (U) were used. Strand breaks were measured by hydroxyapatite chromatography of alkaline unwound DNA (overall strand breaks). Results showed that DNA damage occurs as a function of dose, of kinetic energy and of LET. For particles having the same LET the severity of the DNA damage increases with dose. For a given particle dose, as the LET rises, the numbers of DNA strand breaks increase to a maximum and then reach a plateau or decrease. Repair kinetics depend on the fluence (irradiation dose). At any LET value, repair is much slower after heavy ion exposure than after X-irradiation. For ions with an LET of less than 10,000 keV micrometers-1 more than 90 percent of the strand breaks induced are repaired within 24 hours. At higher particle fluences, especially for low energetic particles with a very high local density of energy deposition within the particle track, a higher proportion of non-rejoined breaks is found, even after prolonged periods of incubation. At the highest LET value (16,300 keV micrometers-1) no significant repair is observed. These LET-dependencies are consistent with the current mechanistic model for radiation induced cataractogenesis which postulates that genomic damage to the surviving fraction of epithelial cells is responsible for lens opacification.     

The detection of energetic cometary and solar particles by the EPONA instrument on the Giotto mission

EPONA is an energetic particle detector system incorporating totally depleted silicon surface barrier layer detectors. Active and passive background shielding will be employed and, by applying various techniques, particles of different species, including electrons, protons, alpha particles and pick-up ions of cometary origin may be detected over a wide spectrum of energies extending from the tens of KeV into the MeV range.

The instrument can operate in two modes namely (a) in a cruise phase or storage mode and (b) in a real time mode. During the real time mode, observations at high spatial (octosectoring) and temporal (0.5s) resolution in the cometary environment permit studies to be made of accelerated particles at the bow shock and/or in the tail of the comet. In conjunction with magnetic field measurements on board Giotto, observations of energetic electrons and their anisotropies can determine whether the magnetic field lines in the cometary tail are open or closed. Further, the absorption of low energy solar particles in the cometary atmosphere can be measured and such data would provide an integral value of the pertaining gas and dust distribution. Solar particle background measurements during encounter may also be used to correct the measurements of other spacecraft borne instruments potentially vulnerable to such radiation.

Solar particle flux measurements, obtained during the cruise phase will, when combined with simultaneous observations made by other spacecraft at different heliographic longitudes, provide information concerning solar particle propagation in the corona and in interplanetary space.     

Charged-particle mutagenesis II. Mutagenic effects of high energy charged particles in normal human fibroblasts.

The biological effects of high LET charged particles are a subject of great concern with regard to the prediction of radiation risk in space. In this report, mutagenic effects of high LET charged particles are quantitatively measured using primary cultures of human skin fibroblasts, and the spectrum of induced mutations are analyzed. The LET of the charged particles ranged from 25 KeV/micrometer to 975 KeV/micrometer with particle energy (on the cells) between 94-603 MeV/u. The X-chromosome linked hypoxanthine guanine phosphoribosyl transferase (hprt) locus was used as the target gene. Exposure to these high LET charged particles resulted in exponential survival curves; whereas, mutation induction was fitted by a linear model. The Relative Biological Effect (RBE) for cell-killing ranged from 3.73 to 1.25, while that for mutant induction ranged from 5.74 to 0.48. Maximum RBE values were obtained at the LET of 150 keV/micrometer. The inactivation cross-section (alpha i) and the action cross-section for mutant induction (alpha m) ranged from 2.2 to 92.0 micrometer2 and 0.09 to 5.56 x 10(-3) micrometer2, respectively. The maximum values were obtained by 56Fe with an LET of 200 keV/micrometer. The mutagenicity (alpha m/alpha i) ranged from 2.05 to 7.99 x 10(-5) with the maximum value at 150 keV/micrometer. Furthermore, molecular analysis of mutants induced by charged particles indicates that higher LET beams are more likely to cause larger deletions in the hprt locus.     

The characteristics of sharp (small-scale) boundaries of solar wind plasma and magnetic field structures

We investigate properties of large (>20%) and sharp (<10 min) solar wind ion flux changes using INTERBALL-1 and WIND plasma and magnetic field measurements from 1996 to 1999. These ion flux changes are the boundaries of small-scale and middle-scale solar wind structures. We describe the behavior of the solar wind velocity, temperature and interplanetary magnetic field (IMF) during these sudden flux changes. Many of the largest ion flux changes occur during periods when the solar wind velocity is nearly constant, so these are mainly plasma density changes. The IMF magnitude and direction changes at these events can be either large or small. For about 55% of the ion flux changes, the sum of the thermal and magnetic pressure are in balance across the boundary. In many of the other cases, the thermal pressure change is significantly more than the magnetic pressure change. We also attempted to classify the types of discontinuities observed.     

Annual solar UV exposure and biological effective dose rates on the Martian surface.

The ultraviolet (UV) environment of Mars has been investigated to gain an understanding of the variation of exposure throughout a Martian year, and link this flux to biological effects and possible survival of organisms at the Martian surface. To gain an idea of how the solar UV radiation varies between different regions, including planned landing sites of two future Mars surface missions, we modelled the total solar UV surface flux throughout one Martian year for two different dust scenarios. To understand the degree of solar UV stress on micro-organisms and/or molecules essential for life on the surface of Mars, we also calculated the biologically effective dose (BED) for T7 and Uracil in relevant wavelength regions at the Martian surface as a function of season and latitude, and discuss the biological survival rates in the presence of Martian solar UV radiation. High T7/Uracil BED ratios indicate that even at high latitudes where the UV flux is significantly reduced, the radiation environment is still hostile for life due to the persisting UV-C component of the flux.     

Problems with the concept of the inflationary universe

The picture of an exponentially increasing, “inflationary” phase of the early universe (Guth 1981; Linde 1982; Albrecht and Steinhardt 1982) may point the way to an understanding of our present universe without reference to extremely specific initial conditions. The model rests, however, on several assumptions which deserve critical examination.     

Solar UV irradiance variation during cycles 22 and 23

The Solar Ultraviolet Spectral Irradiance Monitor (SUSIM) aboard the Upper Atmosphere Research Satellite (UARS) has been measuring solar UV irradiances since October 1991, a period which includes the decline of solar cycle 22 followed by the rise of cycle 23. Daily solar measurements include scans over the wavelength range 115–410 nm at 1.1 nm resolution. As expected, the measured time series of UV irradiances exhibit strong periodicities in solar cycle and solar rotation. For all wavelengths, the UV irradiance time series are similar to that of the Mg II core-to-wing ratio. During solar cycle 22, the irradiance of the strong Ly- line varied by more than a factor of two. The peak-to-peak irradiance variation declined with increasing wavelength, reaching 10% just below the Al edge at 208 nm. Between the Al edge and 250 nm the variation was 6–7%. Above 250 nm, the variation declines further until none is observed above 290 nm. Preliminary results for the first portion of cycle 23 indicate that the far UV below the Al edge is rising at about the same rate as the Mg II index while the irradiances in the Ly- emission line and for wavelengths longer than the Al edge are rising more slowly — even after accounting for the lower level of activity of cycle 23.     

The effect of gravity on surface temperature and net photosynthetic rate of plant leaves.

To clarify the effects of gravity on heat/gas exchange between plant leaves and the ambient air, the leaf temperatures and net photosynthetic rates of plant leaves were evaluated at 0.01, 1.0, 1.5 and 2.0 G of 20 seconds each during a parabolic airplane flight. Thermal images of leaves were captured using infrared thermography at an air temperature of 26 degrees C, a relative humidity of 15% and an irradiance of 260 W m-2. The net photosynthetic rates were determined by using a chamber method with an infrared gas analyzer at an air temperature of 20 degrees C, a relative humidity of 50% and a photosynthetic photon flux of 0.5 mmol m-2 s-1. The mean leaf temperature increased by 1 degree C and the net photosynthetic rate decreased by 13% with decreasing gravity levels from 1.0 to 0.01 G. The leaf temperature decreased by 0.5 degree C and the net photosynthetic rate increased by 7% with increasing gravity levels from 1.0 to 2.0 G. Heat/gas exchanges between leaves and the ambient air were more retarded at lower gravity levels. A restricted free air convection under microgravity conditions in space would limit plant growth by retarding heat and gas exchanges between leaves and the ambient air.