A three-dimensional plasma and energetic particle investigation for the wind spacecraft

This instrument is designed to make measurements of the full three-dimensional distribution of suprathermal electrons and ions from solar wind plasma to low energy cosmic rays, with high sensitivity, wide dynamic range, good energy and angular resolution, and high time resolution. The primary scientific goals are to explore the suprathermal particle population between the solar wind and low energy cosmic rays, to study particle accleration and transport and wave-particle interactions, and to monitor particle input to and output from the Earth's magnetosphere.Three arrays, each consisting of a pair of double-ended semi-conductor telescopes each with two or three closely sandwiched passivated ion implanted silicon detectors, measure electrons and ions above 20 keV. One side of each telescope is covered with a thin foil which absorbs ions below 400 keV, while on the other side the incoming <400 keV electrons are swept away by a magnet so electrons and ions are cleanly separated. Higher energy electrons (up to 1 MeV) and ions (up to 11 MeV) are identified by the two double-ended telescopes which have a third detector. The telescopes provide energy resolution of E/E0.3 and angular resolution of 22.5°×36°, and full 4 steradian coverage in one spin (3 s).Top-hat symmetrical spherical section electrostatic analyzers with microchannel plate detectors are used to measure ions and electrons from 3 eV to 30 keV. All these analyzers have either 180° or 360° fields of view in a plane, E/E0.2, and angular resolution varying from 5.6° (near the ecliptic) to 22.5°. Full 4 steradian coverage can be obtained in one-half or one spin. A large and a small geometric factor analyzer measure ions over the wide flux range from quiet-time suprathermal levels to intense solar wind fluxes. Similarly two analyzers are used to cover the wide range of electron fluxes. Moments of the electron and ion distributions are computed on board.In addition, a Fast Particle Correlator combines electron data from the high sensitivity electron analyzer with plasma wave data from the WAVE experiment (Bougeretet al., in this volume) to study wave-particle interactions on fast time scales. The large geometric factor electron analyzer has electrostatic deflectors to steer the field of view and follow the magnetic field to enhance the correlation measurements.     

Clinorotation reduces number, but not size, of cartilaginous nodules formed in micromass cultures of mouse limbbud cells.

In previous studies we used a ground based model to investigate the cellular responses to microgravity by exposing micromass cultures of embryonic limb cells to simulated weightlessness on a clinostat. Cultures set up in T-flasks and rotated at 30 rpm showed that clinostatted cultures had less chondrocyte differentiation than stationary or rotation controls, as assessed by number of nodules/culture stained with cartilage specific Alcian blue. In the current study, nodule size and shape of these nodules was assessed by interactive measurement of area, perimeter, circularity, and equivalent diameters, using the Optimas imaging software. Results show no significant difference in any of the measurements, indicating that clinorotation has no effect on expansion of the nodules either by differentiation of cells within the nodule, or by recruitment of cells into the nodule. The reduction in number of nodules without an alteration in size and shape indicates that the effect of simulated microgravity is to reduce the cell interactions required for the initial condensation of cells into a nodule, probably by interference with cell adhesion molecules.