Repair of DNA double-strand breaks and cell killing by charged particles.

It has been suggested that it is not simple double-strand breaks (dsb) but the non-reparable breaks which correlate well with the high biological effectiveness of high LET radiations for cell killing (Kelland et al., 1988; Radford, 1986). We have compared the effects of charged particles on cell death in 3 pairs of cell lines which are normal or defective in the repair of DNA dsbs. For the cell lines SL3-147, M10, and SX10 which are deficient in DNA dsb repair, RBE values were close to unity for cell killing induced by charged particles with linear energy transfer (LET) up to 200 keV/micrometer and were even smaller than unity for the LET region greater than 300 keV/micrometer. The inactivation cross section (ICS) increased with LET for all 3 pairs. The ICS of dsb repair deficient mutants was always larger than that of their parents for all the LET ranges, but with increasing LET the difference in ICS between the mutant and its parent became smaller. Since a small difference in ICS remained at LET of about 300 keV/micrometer, dsb repair may still take place at this high LET, even if its role is apparently small. These results suggest that the DNA repair system does not play a major role in protection against the attack of high LET radiations and that a main muse of cell death is non-reparable dsb which are produced at a higher yield compared with low LET radiations. No correlation was observed between DNA content or nuclear area and ICS.     

Adaptive detection schemes in compound-Gaussian clutter

Radar detection of coherent pulse trains embedded in compound-Gaussian disturbance with partially known statistics is discussed. We first give a thorough derivation of two recently proposed adaptive detection structures. Next, we derive a different detection scheme exploiting the assumption that the clutter is wide-sense stationary. Resorting to the theory of circulant matrices, in fact, we demonstrate that the estimation of the structure of the clutter covariance matrix can be reduced to the estimation of its eigenvalues, which in turn can be (efficiently) done via fast Fourier transform codes. After a thorough performance assessment, mostly carried on via computer simulations, the results show that the newly proposed detector achieves better performance than the two previously introduced adaptive detectors. Moreover, a sensitivity analysis shows that, even though this detector does not strictly guarantee the constant false alarm rate property with respect to the clutter covariance matrix, it is robust, in the sense that its performance is only slightly affected by variations in the clutter temporal correlation     

Empirical modeling of the total electron content in the ionosphere

A method of modeling the total electron content (TEC) based on the semi-empirical ionospheric model developed in Irkutsk State University is suggested. Comparison with the Klobuchar model has shown that the proposed method provides a more accurate presentation of TEC. A conclusion is drawn that the use of this method for compensation of the ionospheric error in single-frequency navigation receivers would lead to a substantial increase in the accuracy of their positioning.     

Solar Models with Non-Standard Chemical Composition

The OPAL monochromatic opacity tables are used to evaluate the impact of a non-standard chemical composition on solar models. A calibrated solar model with consistent diffusion including the effect of radiative forces and ionization on drift velocities is presented. It is shown that surface abundances are predicted to change slightly more than in traditional solar models where these refinements are not included. All elements included in the model settle at similar rates which is reflected in the relative variation in surface abundances ranging from 7.5% for calcium to 8.8% for argon. The structural difference between the consistent model and the traditional model is small, with a maximum effect of 0.3% for the isothermal sound speed at the base of the convection zone. The settling of CNO is only marginally affected. Opacity profiles have also been calculated with varying abundances for volatile elements, for which the abundances are poorly known, and other selected elements. It is shown that if one allows a 10% variation of these elements individually one can expect a peak Rosseland mean opacity variation of 3% for oxygen, a little less 2% for Si and Ne, and around 1% for Mg and S in the radiative zone. Other light metals and volatile elements have no significant impact on the opacity. This revised version was published online in June 2006 with corrections to the Cover Date.     

Bearings-only tracking for maneuvering sources

Classical bearings-only target-motion analysis (TMA) is restricted to sources with constant motion parameters (usually position and velocity). However, most interesting sources have maneuvering abilities, thus degrading the performance of classical TMA. In the passive sonar context a long-time source-observer encounter is realistic, so the source maneuver possibilities may be important in regard to the source and array baseline. This advocates for the consideration and modeling of the whole source trajectory including source maneuver uncertainty. With that aim, a convenient framework is the hidden Markov model (HMM). A basic idea consists of a two-levels discretization of the state-space. The probabilities of position transition are deduced from the probabilities of velocity transitions which, themselves, are directly related to the source maneuvering capability. The source state sequence estimation is achieved by means of classical dynamic programming (DP). This approach does not require any prior information relative to the source maneuvers. However, the probabilistic nature of the source trajectory confers a major role to the optimization of the observer maneuvers. This problem is then solved by using the general framework of the Markov decision process (MDP)     

Sedimenting particles and swimming micro-organisms in a rotating fluid.

Experiments and calculations on the trajectories of micron-sized spheres, suspended in a fluid that fills a dosed container which rotates about an axis perpendicular to g, relate to the planning and interpretation of clinostat experiments. For low Reynolds number motion, the orbits are nearly circular, the radius being inversely proportional to the rotation rate. The swimming direction of micro-organisms can be affected by light, gravity, vorticity etc. The trajectories of algae swimming in steadily rotating environments have been observed and compared with theoretical predictions for ideal gyrotactic micro-organisms, thus providing some insights into the mechanisms of gravitaxis, gyrotaxis and the behaviour of the cells.     

Maps of hydrogen isotopes at low altitudes in the inner zone from sampex observations

The PET instrument aboard the SAMPEX satellite has provided us with long-term intra-calibrated observations of geomagnetically trapped protons and deuterons in the inner zone, suitable for use in constraining the low-altitude portions of radiation belt models being developed as successors to AP-8. These observations have been summarized elsewhere (Looper et al., 1996). Here we report a detection of geomagnetically-trapped tritium at energies from 14 to 35 MeV/nuc below L = 1.2, at about 1/8 the flux of deuterium previously reported at that location and at similar energy per nucleon. We also demonstrate the utility of the SAMPEX/PET observations for measuring the east-west anisotropy in the trapped particle flux at low altitudes, which is due to displacement of particle gyrocenters from the position of observation in a region of strong flux gradients. This anisotropy is implicitly ignored in omnidirectional radiation-flux models, but it can be important to mission planners considering how to distribute shielding over the surface of oriented spacecraft in low Earth orbit.     

Effect of track structure and radioprotectors on the induction of oncogenic transformation in murine fibroblasts by heavy ions

The oncogenic potential of high-energy ⁵⁶Fe particles (1 GeV/nucleon) accelerated with the Alternating Gradient Synchrotron at the Brookhaven National Laboratory was examined utilizing the mouse C3H 10T1/2 cell model. The dose-averaged LET for high-energy ⁵⁶Fe is estimated to be 143 keV/μm with the exposure conditions used in this study. For ⁵⁶Fe ions, the maximum relative biological effectiveness (RBE_max) values for cell survival and oncogenic transformation were 7.71 and 16.5 respectively. Compared to 150 keV/μm ⁴He nuclei, high-energy ⁵⁶Fe nuclei were significantly less effective in cell killing and oncogenic induction. The prostaglandin E₁ analog misoprostol, an effective oncoprotector of C3H 10T1/2 cells exposed to X rays, was evaluated for its potential as a radioprotector of oncogenic transformation with high-energy ⁵⁶Fe. Exposure of cells to misoprostol did not alter ⁵⁶Fe cytotoxicity or the rate of ⁵⁶Fe-induced oncogenic transformation.     

Clinorotation reduces number, but not size, of cartilaginous nodules formed in micromass cultures of mouse limbbud cells.

In previous studies we used a ground based model to investigate the cellular responses to microgravity by exposing micromass cultures of embryonic limb cells to simulated weightlessness on a clinostat. Cultures set up in T-flasks and rotated at 30 rpm showed that clinostatted cultures had less chondrocyte differentiation than stationary or rotation controls, as assessed by number of nodules/culture stained with cartilage specific Alcian blue. In the current study, nodule size and shape of these nodules was assessed by interactive measurement of area, perimeter, circularity, and equivalent diameters, using the Optimas imaging software. Results show no significant difference in any of the measurements, indicating that clinorotation has no effect on expansion of the nodules either by differentiation of cells within the nodule, or by recruitment of cells into the nodule. The reduction in number of nodules without an alteration in size and shape indicates that the effect of simulated microgravity is to reduce the cell interactions required for the initial condensation of cells into a nodule, probably by interference with cell adhesion molecules.     

New evidence for strong non-thermal effects in Tycho’s supernova remnant

For the case of Tycho’s supernova remnant (SNR), we present the relation between the blast wave and contact discontinuity radii calculated within the nonlinear kinetic theory of cosmic ray (CR) acceleration in SNRs. It is demonstrated that these radii are confirmed by recently published Chandra measurements which show that the observed contact discontinuity radius is very close to the shock radius. Therefore a consistent explanation of these observations can be given in terms of efficient CR acceleration which makes the medium more compressible.