Clinorotation reduces number, but not size, of cartilaginous nodules formed in micromass cultures of mouse limbbud cells.

In previous studies we used a ground based model to investigate the cellular responses to microgravity by exposing micromass cultures of embryonic limb cells to simulated weightlessness on a clinostat. Cultures set up in T-flasks and rotated at 30 rpm showed that clinostatted cultures had less chondrocyte differentiation than stationary or rotation controls, as assessed by number of nodules/culture stained with cartilage specific Alcian blue. In the current study, nodule size and shape of these nodules was assessed by interactive measurement of area, perimeter, circularity, and equivalent diameters, using the Optimas imaging software. Results show no significant difference in any of the measurements, indicating that clinorotation has no effect on expansion of the nodules either by differentiation of cells within the nodule, or by recruitment of cells into the nodule. The reduction in number of nodules without an alteration in size and shape indicates that the effect of simulated microgravity is to reduce the cell interactions required for the initial condensation of cells into a nodule, probably by interference with cell adhesion molecules.     

New evidence for strong non-thermal effects in Tycho’s supernova remnant

For the case of Tycho’s supernova remnant (SNR), we present the relation between the blast wave and contact discontinuity radii calculated within the nonlinear kinetic theory of cosmic ray (CR) acceleration in SNRs. It is demonstrated that these radii are confirmed by recently published Chandra measurements which show that the observed contact discontinuity radius is very close to the shock radius. Therefore a consistent explanation of these observations can be given in terms of efficient CR acceleration which makes the medium more compressible.     

Tissue responses to low protracted doses of high LET radiations or photons: early and late damage relevant to radio-protective countermeasures.

Early and late murine tissue responses to single or fractionated low doses of heavy charged particles, fission-spectrum neutrons or gamma rays are considered. Damage to the hematopoietic system is emphasized, but results on acute lethality, host response to challenge with transplanted leukemia cells and life-shortening are presented. Low dose rates per fraction were used in some neutron experiments. Split-dose lethality studies (LD 50/30) with fission neutrons indicated greater accumulation of injury during a 9 fraction course (over 17 days) than was the case for gamma-radiation. When total doses of 96 or 247 cGy of neutrons or gamma rays were given as a single dose or in 9 fractions, a significant sparing effect on femur CFU-S depression was observed for both radiation qualities during the first 11 days, but there was not an earlier return to normal with dose fractionation. During the 9 fraction sequence, a significant sparing effect of low dose rate on CFU-S depression was observed in both neutron and gamma-irradiated mice. CFU-S content at the end of the fractionation sequence did not correlate with measured LD 50/30. Sustained depression of femur and spleen CFU-S and a significant thrombocytopenia were observed when a total neutron dose of 240 cGy was given in 72 fractions over 24 weeks at low dose rates. The temporal aspects of CFU-S repopulation were different after a single versus fractionated neutron doses. The sustained reduction in the size of the CFU-S population was accompanied by an increase in the fraction in DNA synthesis. The proliferation characteristics and effects of age were different for radial CFU-S population closely associated with bone, compared with the axial population that can be readily aspirated from the femur. In aged irradiated animals, the CFU-S proliferation/redistribution response to typhoid vaccine showed both an age and radiation effect. After high single doses of neutrons or gamma rays, a significant age- and radiation-related deficiency in host defense mechanisms was detected by a shorter mean survival time following challenge with transplantable leukemia cells. Comparison of dose-response curves for life shortening after irradiation with fission-spectrum neutrons or high energy silicon particles indicated high initial slopes for both radiation qualities at low doses, but for higher doses of silicon, the effect per Gy decreased to a value similar to that for gamma rays. The two component life-shortening curve for silicon particles has implications for the potential efficacy of radioprotectants. Recent studies on protection against early and late effects by aminothiols, prostaglandins, and other compounds are discussed.     

On the Radar Echo from Aircraft

The nonstationarity of the low-frequency radar echo envelope observed from an aircraft is shown and a simple model of the echo is suggested. This model, devised specifically for simulation studies using computers, reproduces the characteristics of the observed envelope, demonstrating the correlation between the observed nonstationarity and aircraft motion relative to the receiver.     

Plasma observations in the auroral and polar cap region

The last decade has seen a period of rapid growth in our understanding of the processes which occur in the auroral regions. Much of our understanding is based on the copious new observations which have been made available in the auroral community. The present work is a short overview of the plasma conditions which obtain throughout much of the auroral region. It covers the diffuse and discrete auroral electron precipitation in the morning and evening oval, cusp, and polar cap. The ionospheric ion outflow throughout the high latitude regime is also described and related to the electron observations.     

Propagation and interaction of interplanetary transient disturbances. Numerical simulations

We study the heliocentric evolution of ICME-like disturbances and their associated transient forward shocks (TFSs) propagating in the interplanetary (IP) medium comparing the solutions of a hydrodynamic (HD) and magnetohydrodynamic (MHD) models using the ZEUS-3D code [Stone, J.M., Norman, M.L., 1992. Zeus-2d: a radiation magnetohydrodynamics code for astrophysical flows in two space dimensions. i – the hydrodynamic algorithms and tests. Astrophysical Journal Supplement Series 80, 753–790]. The simulations show that when a fast ICME and its associated IP shock propagate in the inner heliosphere they have an initial phase of about quasi-constant propagation speed (small deceleration) followed, after a critical distance (deflection point), by an exponential deceleration. By combining white light coronograph and interplanetary scintillation (IPS) measurements of ICMEs propagating within 1 AU [Manoharan, P.K., 2005. Evolution of coronal mass ejections in the inner heliosphere: a study using white-light and scintillation images. Solar Physics 235 (1–2), 345–368], such a critical distance and deceleration has already been inferred observationally. In addition, we also address the interaction between two ICME-like disturbances: a fast ICME 2 overtaking a previously launched slower ICME 1. After interaction, the leading ICME 1 accelerates and the tracking ICME 2 decelerates and both ICMEs tend to arrive at 1 AU having similar speeds. The 2-D HD and MHD models show similar qualitative results for the evolution and interaction of these disturbances in the IP medium.     

An improved Green's function for ion beam transport.

Ion beam transport theory allows testing of material transmission properties in the laboratory environment generated by particle accelerators. This is a necessary step in materials development and evaluation for space use. The approximations used in solving the Boltzmann transport equation for the space setting are often not sufficient for laboratory work and those issues are the main emphasis of the present work. In consequence, an analytic solution of the linear Boltzmann equation is pursued in the form of a Green's function allowing flexibility in application to a broad range of boundary value problems. It has been established that simple solutions can be found for high charge and energy (HZE) ions by ignoring nuclear energy downshifts and dispersion. Such solutions were found to be supported by experimental evidence with HZE ion beams when multiple scattering was added. Lacking from the prior solutions were range and energy straggling and energy downshift with dispersion associated with nuclear events. Recently, we have found global solutions including these effects providing a broader class of HZE ion solutions.