Plant cell plasma membrane structure and properties under clinostatting.

Structural-functional organization of plasma membrane of pea roots seedling was investigated by methods of chemiluminescence, fluorescence probes, chromatography and freeze-fracture studies under normal conditions and clinostatting. Phase character of lipid peroxidation intensity was fixed. The initial phase of this process is characterized by lipid peroxidation decreasing with its next induction. The primary changes depending on free-radical mechanisms of lipid peroxidation were excellently revealed by chemiluminescence. Plasmalemma microviscosity increased on the average of 15-20% under microgravity at the initial stages of its phenomenon. There were major changes of phosphatidilcholine and phosphatidilethanolamine contents. The total quantity of phospholipids remained rather stable. Changes of phosphatide acid concentration point to degradation and phospholipids biosynthesis. There were increases of unsaturated fatty acids mainly at the expense of linoleic and linolenic acids and also a decrease of saturated fatty acid content at the expense of palmitic and stearic acids. Unsaturation index of fatty acids increased as well. On the whole fatty acid composition was variable in comparison with phospholipids. Probably it is one of mechanisms of maintaining of microviscosity within definite limits. Considerable structural changes in organization of plasmalemma protein-lipid complex were not revealed by the freeze-fracture studies.     

Control of complex nonlinear vibrations of sandwich beams

Complex vibrations of the Bernoulli-Euler sandwich beams are considered. The solution is performed by the numerical methods of finite elements in the Bubnov-Galerkin form and finite differences of the second order of accuracy. The approaches for control of chaotic vibrations in the Bernoulli-Euler sandwich beams are proposed.     

The effect of total solar eclipse of October 3, 2005, on the total electron content over Europe

GPS observations from EUREF permanent GPS network were used to observe the response of TEC (Total Electron Content) to the total solar eclipse on October 3, 2005, under quiet geomagnetic conditions of the daytime ionosphere. The effect of the eclipse was detected in diurnal variations and more distinctly in the variations of TEC along individual satellite passes. The trough-like variations with a gradual decrease and followed by an increase of TEC at the time of the eclipse were observed over a large region. The depression of TEC amounted to 3–4 TECU. The maximum depression was observed over all stations located at the maximum path of the solar eclipse. The delay of a minimum level of TEC with respect to the maximum phase of the eclipse was about 20–30 min.     

Initial performance assessment of hybrid inflatable structures

Inflatable technology for space applications is under continual development and advances in high strength fibers and rigidizable materials have pushed the limitations of these structures. This has lead to their application in deploying large-aperture antennas, reflectors and solar sails. However, many significant advantages can be achieved by combining inflatable structures with structural stiffeners such as tape springs. These advantages include control of the deployment path of the structure while it is inflating (a past weakness of inflatable structure designs), an increased stiffness of the structure once deployed and a reduction in the required inflation volume. Such structures have been previously constructed at the Jet Propulsion Laboratory focusing on large scale booms. However, due to the high efficiency of these designs they are also appealing to small satellite systems.This article outlines ongoing research work performed at the University of Southampton into the field of small satellite hybrid inflatable structures. Inflatable booms have been constructed and combined with tape spring reinforcements to create simple hybrid structures. These structures have been subjected to bending tests and compared directly to an equivalent inflatable tube without tape spring reinforcement. This enables the stiffness benefits to be determined with respect to the added mass of the tape springs. The paper presents these results, which leads to an initial performance assessment of these structures.     

DNA-lesion and cell death by alpha-particles and nitrogen ions.

When the natural logarithm of the surviving fraction is plotted against the dose of radiation, curves with shoulders at relatively high survival levels are obtained after gamma-rays. The curves were practically linear in case of HMV-I and HA-1 cells irradiated by charged particle beams. These cells were derived from human malignant melanoma and Chinese hamster cells, respectively. The amount of DNA single strand breaks (ssb) by gamma-rays or nitrogen-ions (LET=530KeV/micrometers) in HMV-I cells increases linearly with increment in dose, when the ssb is detected using the alkaline elution technique. There is no close relationship between the dose-response curve of the ssb and the dose-survival curves after gamma-rays or N-ions. The amount of DNA double strand breaks (dsb) by gamma-rays increases quadratically with increment of dose, in both HMV-I cells and HA-1 cells, when the dsb is detected using the neutral elution technique. The survival fraction for HA-1 cells is slightly higher than that for HMV-I cells, at the same dose, and the amount of dsb for HA-1 cells is considerably greater than that for HMV-I cells. These results suggest that the radiosensitivities to gamma-rays in different cell lines do not correspond to the number of DNA strand breaks. The amount of both non-repairable ssb and dsb also increases quadratically with increment of dose for gamma-rays and almost linearly with increment of dose for N-ions and alpha-particles (LET=36keV/micrometers for HA-1 cells and LET=77keV/micrometers for HMV-I cells). The dose-response curves for non-repairable dsb in case of these radiations seemed to mirror image the dose-survival curves for these radiations, in both cell lines. The number of non-repairable DNA strand breaks in the two cell lines, at the same level of survival was much the same. These results show the close relationship between the induction of non-repairable DNA strand breaks and cell killing.     

Ultrastructural and growth indices of Chlorella culture in multicomponent aquatic systems under space flight conditions.

Submicroscopic organization of Chlorella cells cultivated under space flight conditions in three-component aquatic system has been studied. Comparison of the experimental cells with that of the controls revealed certain rearrangements of cell organelles particularly, a reduction in the amount of reserve polysaccharides in chloroplasts, increase of cell vacuolization and mitochondrion volume, complication configuration of plasmalemma evaginations and invaginations, and also disturbances in the process of cytokinesis. More over an increase in the number of Chlorella cells infected by bacteria was shown in the experimental variant. No considerable differences were established in the growth characteristics of the experimental and control populations. A comparative cytological analysis revealed general regularities of organelles in Chlorella cells cultivated under space flight condition in the uni- and multicomponent systems.     

A demonstration of sub meter GPS orbit determination and highprecision user positioning

High-accuracy orbits have been determined for satellites of the Global Positioning System (GPS), with submeter orbit accuracy demonstrated for two well-tracked satellites. Baselines of up to 2000 km in North America determined with the GPS orbits shows daily repeatability of 0.3-2 parts in 10⁸ and agree with very long baseline interferometry (VLBI) solutions at the level of 1.5 parts in 10 ⁸. Tests used to assess orbit accuracy include orbit repeatability from independent data sets, orbit prediction, ground baseline determination, and formal errors. One satellite tracked for eight hours each day shows RMS errors below 1 m even when predicted more than three days outside of a 1-week data arc. These results demonstrate the powerful relative positioning capability available from differential GPS tracking. Baselines have also been estimated between Florida and sites in the Caribbean region over 1000 km away, with daily repeatability of 1-4 parts in 10⁸. The best orbit estimation strategies included data arcs of 1-2 weeks, process noise models for tropospheric fluctuations, combined processing of GPS carrier phase and pseudorange data, and estimation of GPS solar pressure coefficients     

Determination of the Turbulent Diffusion Coefficient in the Plasma Sheet Using the Project INTERBALL Data

The results of an analysis of velocity fluctuations in the plasma sheet of the Earth's magnetotail measured onboard INTERBALL Tail Probe satellite are presented. The hodographs of the velocity in directions (Y, Z) and correlation functions are presented for a number of passages when the satellite was in the plasma sheet for a long time. The turbulent diffusion coefficients are calculated. A comparison of the obtained diffusion coefficients with those predicted theoretically in [1] is carried out. It is shown that the results of observations confirm theoretical predictions.     

High LET-induced H2AX phosphorylation around the Bragg curve.

We investigated the spatial distribution of the induction of the phosphorylated form of the histone protein H2AX (gamma-H2AX), known to be activated by DSBs. Following irradiation of human fibroblast cells with 600 MeV/nucleon silicon and 600 MeV/nucleon iron ions we observed the formation of gamma-H2AX aggregates in the shape of streaks stretching over several micrometers in an x/y plane. Polyethylene shielding was used to achieve a Bragg curve distribution with beam geometry parallel to the monolayer of cells. We present data that highlights the formation of immunofluorescent gamma-H2AX tracks showing the ion trajectories across the Bragg peak of irradiated human fibroblast cells. Qualitative analyses of these distributions indicated potentially increased clustering of DNA damage before the Bragg peak, enhanced gamma-H2AX distribution at the peak, and provided visual evidence of high-linear energy transfer particle traversal of cells beyond the Bragg peak in agreement with one-dimensional transport approximations. Spatial assessment of gamma-H2AX fluorescence may provide direct insights into DNA damage across the Bragg curve for high charge and energy ions including the biological consequences of shielding and possible contributors to bystander effects.