Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells.

The cytoskeleton is a complex network of fibers that is sensitive to environmental factors including microgravity and altered gravitational forces. Cellular functions such as transport of cell organelles depend on cytoskeletal integrity; regulation of cytoskeletal activity plays a role in cell maintenance, cell division, and apoptosis. Here we report cytoskeletal and mitochondria alterations in cultured human lymphocyte (Jurkat) cells after exposure to spaceflight and in insect cells of Drosophila melanogaster (Schneider S-1) after exposure to conditions created by clinostat rotation. Jurkat cells were flown on the space shuttle in Biorack cassettes while Schneider S-1 cells were exposed to altered gravity forces as produced by clinostat rotation. The effects of both treatments were similar in the different cell types. Fifty percent of cells displayed effects on the microtubule network in both cell lines. Under these experimental conditions mitochondria clustering and morphological alterations of mitochondrial cristae was observed to various degrees after 4 and 48 hours of culture. Jurkat cells underwent cell divisions during exposure to spaceflight but a large number of apoptotic cells was also observed. Similar results were obtained in Schneider S-1 cells cultured under clinostat rotation. Both cell lines displayed mitochondria abnormalities and mitochondria clustering toward one side of the cells which is interpreted to be the result of microtubule disruption and failure of mitochondria transport along microtubules. The number of mitochondria was increased in cells exposed to altered gravity while cristae morphology was severely affected indicating altered mitochondria function. These results show that spaceflight as well as altered gravity produced by clinostat rotation affects microtubule and mitochondria organization and results in increases in apoptosis. Grant numbers: NAG 10-0224, NAG2-985.     

Imaging the solar corona in the EUV

The SOHO (SOlar and Heliospheric Observatory) satellite was launched on December 2nd 1995. After arriving at the Earth-Sun (L1) Lagrangian point on February 14th 1996, it began to continuously observe the Sun. As one of the instruments onboard SOHO, the EIT (Extreme ultraviolet Imaging Telescope) images the Sun's corona in 4 EUV wavelengths. The He II filter at 304 Å images the chromosphere and the base of the transition region at a temperature of 5 − 8 × 10⁴ K; the Fe IX–X filter at 171 Å images the corona at a temperature of 1.3 × 10⁶ K; the Fe XII filter at 195 Å images the quiet corona outside coronal holes at a temperature of 1.6 × 10⁶ K; and the Fe XV filter at 284 Å images active regions with a temperature of 2.0 × 10⁶ K. About 5000 images have been obtained up to the present. In this paper, we describe also some aspects of the telescope and the detector performance for application in the observations. Images and movies of all the wavelengths allow a look at different phenomena present in the Sun's corona, and in particular, magnetic field reconnection.     

A cost driven predictive maintenance policy for structural airframe maintenance

Airframe maintenance is traditionally performed at scheduled maintenance stops.The decision to repair a fuselage panel is based on a fixed crack size threshold,which allows to ensure the aircraft safety until the next scheduled maintenance stop.With progress in sensor technology and data processing techniques,structural health monitoring (SHM) systems are increasingly being considered in the aviation industry.SHM systems track the aircraft health state continuously,lead ing to the possibility of planning maintenance based on an actual state of aircraft rather than on a fixed schedule.This paper builds upon a model-based prognostics framework that the authors developed in their previous work,which couples the Extended Kalman filter (EKF) with a first order perturbation (FOP) method.By using the information given by this prognostics method,a novel cost driven predictive maintenance (CDPM) policy is proposed,which ensures the aircraft safety while minimizing the maintenance cost.The proposed policy is formally derived based on the trade-off between probabilities of occurrence of scheduled and unscheduled maintenance.A numerical case study simulating the maintenance process of an entire fleet of aircrafts is imple mented.Under the condition of assuring the same safety level,the CDPM is compared in terms of cost with two other maintenance policies:scheduled maintenance and threshold based SHM maintenance.The comparison results show CDPM could lead to significant cost savings.     

Entrapment of bacteria in fluid inclusions in laboratory-grown halite

Cells of the bacterium Pseudomonas aeruginosa, which were genetically modified to produce green fluorescent protein, were entrapped in fluid inclusions in laboratory-grown halite. The bacteria were used to inoculate NaCl-saturated aqueous solutions, which were allowed to evaporate and precipitate halite. The number, size, and distribution of fluid inclusions were highly variable, but did not appear to be affected by the presence of the bacteria. Many of the inclusions in crystals from inoculated solutions contained cells in populations ranging from two to 20. Microbial attachment to crystal surfaces was neither evident nor necessary for entrapment. Cells occurred exclusively within fluid inclusions and were not present in the crystal matrix. In both the inclusions and the hypersaline solution, the cells fluoresced and twitched, which indicates that the bacteria might have remained viable after entrapment. The fluorescence continued up to 13 months after entrapment, which indicates that little degradation of the bacteria occurred over that time interval. The entrapment, fluorescence, and preservation of cells were independent of the volume of hypersaline solution used or whether the solutions were completely evaporated prior to crystal extraction. The results of this study have a wide range of implications for the long-term survival of microorganisms in fluid inclusions and their detection through petrography. The results also demonstrate the preservation potential for microbes in hypersaline fluid inclusions, which could allow cells to survive harsh conditions of space, the deep geologic past, or burial in sedimentary basins.     

Can Surface Flux Transport Account for the Weak Polar Field in Cycle 23?

To reproduce the weak magnetic field on the polar caps of the Sun observed during the declining phase of cycle 23 poses a challenge to surface flux transport models since this cycle has not been particularly weak. We use a well-calibrated model to evaluate the parameter changes required to obtain simulated polar fields and open flux that are consistent with the observations. We find that the low polar field of cycle 23 could be reproduced by an increase of the meridional flow by 55% in the last cycle. Alternatively, a decrease of the mean tilt angle of sunspot groups by 28% would also lead to a similarly low polar field, but cause a delay of the polar field reversals by 1.5 years in comparison to the observations.     

轴对称跨音速流中无激波超音速区的设计

本文直接从音速线出发计算了一个旋成体跨音速绕流超音速泡中的特征线网。提出了从音速线开拓到一条超音速线的方法,计算了无旋轴对称流的特征线网,发现其特点是不能跨越对称轴线,并对其原因作了初步的讨论。本文的计算结果可应用于物面修形。     

Magnetospheric Science Objectives of the <Emphasis Type="Italic">Juno</Emphasis> Mission

In July 2016, NASA’s Juno mission becomes the first spacecraft to enter polar orbit of Jupiter and venture deep into unexplored polar territories of the magnetosphere. Focusing on these polar regions, we review current understanding of the structure and dynamics of the magnetosphere and summarize the outstanding issues. The Juno mission profile involves (a) a several-week approach from the dawn side of Jupiter’s magnetosphere, with an orbit-insertion maneuver on July 6, 2016; (b) a 107-day capture orbit, also on the dawn flank; and (c) a series of thirty 11-day science orbits with the spacecraft flying over Jupiter’s poles and ducking under the radiation belts. We show how Juno’s view of the magnetosphere evolves over the year of science orbits. The Juno spacecraft carries a range of instruments that take particles and fields measurements, remote sensing observations of auroral emissions at UV, visible, IR and radio wavelengths, and detect microwave emission from Jupiter’s radiation belts. We summarize how these Juno measurements address issues of auroral processes, microphysical plasma physics, ionosphere-magnetosphere and satellite-magnetosphere coupling, sources and sinks of plasma, the radiation belts, and the dynamics of the outer magnetosphere. To reach Jupiter, the Juno spacecraft passed close to the Earth on October 9, 2013, gaining the necessary energy to get to Jupiter. The Earth flyby provided an opportunity to test Juno’s instrumentation as well as take scientific data in the terrestrial magnetosphere, in conjunction with ground-based and Earth-orbiting assets.     

High velocity impact characterization of Al alloys for oblique impacts

This paper describes the experimental and computational analyses of a high velocity aluminum projectile impact on an Al6061-T6 spacecraft inner wall at different oblique angles. Al2017-T4 spherical projectiles of 5.56 mm in diameter and 0.25 g in weight were chosen within the velocity range of 1000±200 m/s due to the limitation of the light gas gun. The energy absorbed was calculated by measuring the velocities before and after impact on the inner wall. The energy absorbed by the wall and the remaining energy carried by the projectile helped to estimate the severity of further damage to inner components. Afterwards, validation was done by using the commercially available software LS-DYNA with a dedicated SPH. On average, a 10% energy absorption difference between experimentation and simulation was found. By using C-SCAN, the damage area proportion of the total inner wall to impact penetration hole area was found to be on average 6%, 26% and 53% greater than the projectile cross sectional area for the oblique angle impacts of 30°, 45°, and 60°, respectively. These findings helped to understand the relationship between the oblique impact event and the damage area on a spacecraft inner wall along with space debris cloud propagation and comparison with experimental results using LS-DYNA.