Entrapment of bacteria in fluid inclusions in laboratory-grown halite

Cells of the bacterium Pseudomonas aeruginosa, which were genetically modified to produce green fluorescent protein, were entrapped in fluid inclusions in laboratory-grown halite. The bacteria were used to inoculate NaCl-saturated aqueous solutions, which were allowed to evaporate and precipitate halite. The number, size, and distribution of fluid inclusions were highly variable, but did not appear to be affected by the presence of the bacteria. Many of the inclusions in crystals from inoculated solutions contained cells in populations ranging from two to 20. Microbial attachment to crystal surfaces was neither evident nor necessary for entrapment. Cells occurred exclusively within fluid inclusions and were not present in the crystal matrix. In both the inclusions and the hypersaline solution, the cells fluoresced and twitched, which indicates that the bacteria might have remained viable after entrapment. The fluorescence continued up to 13 months after entrapment, which indicates that little degradation of the bacteria occurred over that time interval. The entrapment, fluorescence, and preservation of cells were independent of the volume of hypersaline solution used or whether the solutions were completely evaporated prior to crystal extraction. The results of this study have a wide range of implications for the long-term survival of microorganisms in fluid inclusions and their detection through petrography. The results also demonstrate the preservation potential for microbes in hypersaline fluid inclusions, which could allow cells to survive harsh conditions of space, the deep geologic past, or burial in sedimentary basins.     

A novel framework for liquid propellant engine’s cooling system design by sensitivity analysis based on RSM and multi-objective optimization using PSO

One of the challenges of combustion chamber and nozzle design in a Liquid Propellant Engine (LPE) is to predict the behavior and performance of the cooling system. Therefore, while designing, the optimization of the cooling system is always of great importance. This paper presents the multi-objective optimization of the LPE’s cooling system. To this end, a novel framework has been developed, resulting from the application of the Response Surface Method (RSM) and the correlation coefficients matrix, sensitivity analysis and the The Particle Swarm Optimization (PSO). based on this method, the input variables, constraints, objective functions, and their surfaces were identified. In terms of multi-optimization algorithms, RSM and PSO are utilized to get global optimum. In conclusion, the methodology capability is to optimize the LPE’s cooling system, 6 percentage increase in total heat transfer and 7 bar decrease cooling system pressure loss, which resulted in a 1.2-seconds increase in the specific impulse of the engine.     

可变速发电机对多机电力系统的稳定性提高的影响

阐述了可变速发电机(ASG)对多机电力系统的稳定性提高有着明显的效果。为了利用电力系统模拟仿真器进行实时仿真，开发了基于个人计算机(PC)的可变速发电机模组。通过将Matlab／Simulink环境下建立的ASG三相瞬时值模型装到PC里的数字处理器(DSP)板上运行，来实现更为详细的实时仿真。通过DSP板上的AD／DA转换接口，对基于PC的ASG模组与电力系统模拟仿真器等实施了物理连接，进行了多机电力系统的实时仿真．所有的仿真结果均显示，引入可变速发电机后，多机电力系统的整体稳定性有了大幅度的提高．     

Effect of Thermal Treatment on CFRP Parts Before and After Adhesive Bonding

Numerous non-destructive techniques are being investigated for assuring quality of the adhesive bonds.The research presented here is focused on non-destructive assessment of carbon fibre reinforced polymer(CFRP)parts.The surface condition directly influences the performance of adhesive bonds.The structural joints should ensure safe usage of a structure.However,some modifications of the surface may lead to weak bond that cannot carry the desired load.This is why there is a search for methods of surface assessment before bonding.Moreover,reliable techniques are required to allow to verify the integrity of the adhesive bond after manufacturing or bonded repair.We focus on the laser induced fluorescence(LIF)method for assessing the surface state.The LIF is a noncontact measurement method.In the context of adhesive bond assessment the electromechanical impedance(EMI)method is studied.The EMI uses surface bonded piezoelectric sensors for excitation and sensing.The investigated samples were made of CFRP layers.The samples were treated at elevated temperatures.The influence of the thermal treatment was studied using LIF.The thermal treatment at 220℃could be clearly distinguishedrom the rest of the considered samples.The thermally treated plates were bonded to untreated plate and then they were measured with the EMI method to study the influence of the treatment on the adhesive bond.The changes of EMI spectra were significant for the treatment at 280 ℃ and for some thermally treated samples that were later contaminated with de-icing fluid.     

The effect of radiation on the long term productivity of a plant based CELSS.

Mutations occur at a higher rate in space than under terrestrial conditions, primarily due to an increase in radiation levels. These mutations may effect the productivity of plants found in a controlled ecological life support system (CELSS). Computer simulations of plants with different ploidies, modes of reproduction, lethality thresholds, viability thresholds and susceptibilities to radiation induced mutations were performed under space normal and solar flare conditions. These simulations identified plant characteristics that would enable plants to retain high productivities over time in a CELSS.     

Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.

The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.     

Application of Gram-Schmidt algorithm to fully adaptive arrays

The Gram-Schmidt orthogonalization (GSO) algorithm has excellent numerical performance and is readily applicable to systolic implementations such as in a field of adaptive cancellation systems. A modified GSO algorithm for a fully adaptive array is proposed and computer simulations show that the proposed algorithm gives superior performance. A systolic implementation of the proposed GSO algorithm for fully adaptive array is presented. A feedback mode GSO algorithm for use with analog weights is also presented and has been shown to have excellent performance in the presence of weight errors     

On adaptive multiband signal detection with the SMI algorithm

In a nonstationary and/or nonhomogeneous interference environment, an adaptive system for target detection may suffer a severe performance degradation due to the lack of a sufficient amount of data from which the system can learn (estimate) the statistics of the environment. The detection performance of an adaptive system, which employs a frequency diversity (multiband) signaling waveform and a multiband sample matrix inversion algorithm (SMI), is analyzed. By comparison with the corresponding single-band system under the chosen system constraint, it is shown that the multiband system can significantly outperform the single band when the amount of data available from a single frequency band is severely limited by the environment     

Variations of the high energy electron flux along the Ulysses trajectory

We describe for the first time the analysis of high energy electrons (above 240 MeV) from the COSPIN/KET experiment onboard Ulysses. The electron time profiles in four energy windows are presented from Oct. 90 to the end of March 94, up to a maximum heliographic latitude of 57 °S. The recovery rates we derived for the electrons are compared to the recovery rates of positively charged particles with the same rigidity.     

SWE,a comprehensive plasma instrument for the WIND spacecraft

The Solar Wind Experiment (SWE) on the WIND spacecraft is a comprehensive, integrated set of sensors which is designed to investigate outstanding problems in solar wind physics. It consists of two Faraday cup (FC) sensors; a vector electron and ion spectrometer (VEIS); a strahl sensor, which is especially configured to study the electron strahl close to the magnetic field direction; and an on-board calibration system. The energy/charge range of the Faraday cups is 150 V to 8 kV, and that of the VEIS is 7 V to 24.8 kV. The time resolution depends on the operational mode used, but can be of the order of a few seconds for 3-D measurements. Key parameters which broadly characterize the solar wind positive ion velocity distribution function will be made available rapidly from the GGS Central Data Handling Facility.