Assessing antibody microarrays for space missions: effect of long-term storage, gamma radiation, and temperature shifts on printed and fluorescently labeled antibodies

Antibody microarrays are becoming frequently used tools for analytical purposes. A key factor for optimal performance is the stability of the immobilized (capturing) antibodies as well as those that have been fluorescently labeled to achieve the immunological test (tracers). This is especially critical for long-distance transport, field testing, or planetary exploration. A number of different environmental stresses may affect the antibody integrity, such as dryness, sudden temperature shift cycles, or, as in the case of space science, exposure to large quantities of the highly penetrating gamma radiation. Here, we report on the effect of certain stabilizing solutions for long-term storage of printed antibody microarrays under different conditions. We tested the effect of gamma radiation on printed and freeze- or vacuum-dried fluorescent antibodies at working concentrations (tracer antibodies), as well as the effect of multiple cycles of sudden and prolonged temperature shifts on the stability of fluorescently labeled tracer antibody cocktails. Our results show that (i) antibody microarrays are stable at room temperature when printed on stabilizing spotting solutions for at least 6 months, (ii) lyophilized and vacuum-dried fluorescently labeled tracer antibodies are stable for more than 9 months of sudden temperature shift cycles (-20°C to 25°C and 50°C), and (iii) both printed and freeze- or vacuum-dried fluorescent tracer antibodies are stable after several-fold excess of the dose of gamma radiation expected during a mission to Mars. Although different antibodies may exhibit different susceptibilities, we conclude that, in general, antibodies are suitable for use in planetary exploration purposes if they are properly treated and stored with the use of stabilizing substances.     

Dust Phenomena Relating to Airless Bodies

Airless bodies are directly exposed to ambient plasma and meteoroid fluxes, making them characteristically different from bodies whose dense atmospheres protect their surfaces from such fluxes. Direct exposure to plasma and meteoroids has important consequences for the formation and evolution of planetary surfaces, including altering chemical makeup and optical properties, generating neutral gas and/or dust exospheres, and leading to the generation of circumplanetary and interplanetary dust grain populations. In the past two decades, there have been many advancements in our understanding of airless bodies and their interaction with various dust populations. In this paper, we describe relevant dust phenomena on the surface and in the vicinity of airless bodies over a broad range of scale sizes from \(\sim10^{-3}~\mbox{km}\) to \(\sim10^{3}~\mbox{km}\), with a focus on recent developments in this field.     

Estimating Radiative Momentum Transfer Through a Thermal Analysis of the Pioneer Anomaly

A methodology based on point-like sources is discussed, enabling a reliable estimate of the acceleration of the Pioneer 10 and 11 probes caused by thermal effects. A sensitivity analysis of the several parameters of the model allows for a clear indication of the possible thermal origin of the so-called Pioneer anomaly.     

Iron meteorites can support the growth of acidophilic chemolithoautotrophic microorganisms

Chemolithoautotrophy based on reduced inorganic minerals is considered a primitive energy transduction system. Evidence that a high number of meteorites crashed into the planet during the early period of Earth history led us to test the ability of iron-oxidizing bacteria to grow using iron meteorites as their source of energy. Here we report the growth of two acidophilic iron-oxidizing bacteria, Leptospirillum ferrooxidans and Acidithiobacillus ferrooxidans, on a piece of the Toluca meteorite as the only source of energy. The alteration of the surface of the exposed piece of meteorite, the solubilization of its oxidized metal constituents, mainly ferric iron, and the formation of goethite precipitates all clearly indicate that iron-meteorite-based chemolithotrophic metabolism is viable.     

Impact of Distance Determinations on Galactic Structure. II. Old Tracers

Here we review the efforts of a number of recent results that use old tracers to understand the build up of the Galaxy. Details that lead directly to using these old tracers to measure distances are discussed. We concentrate on the following: (1) the structure and evolution of the Galactic bulge and inner Galaxy constrained from the dynamics of individual stars residing therein; (2) the spatial structure of the old Galactic bulge through photometric observations of RR Lyrae-type stars; (3) the three-dimensional structure, stellar density, mass, chemical composition, and age of the Milky Way bulge as traced by its old stellar populations; (4) an overview of RR Lyrae stars known in the ultra-faint dwarfs and their relation to the Galactic halo; and (5) different approaches for estimating absolute and relative cluster ages.     

Spiders: water-driven erosive structures in the southern hemisphere of Mars

Recent data from space missions reveal that there are ongoing climatic changes and erosive processes that continuously modify surface features of Mars. We have investigated the seasonal dynamics of a number of morphological features located at Inca City, a representative area at high southern latitude that has undergone seasonal processes. By integrating visual information from the Mars Orbiter Camera on board the Mars Global Surveyor and climatic cycles from a Mars' General Circulation Model, and considering the recently reported evidence for the presence of water-ice and aqueous precipitates on Mars, we propose that a number of the erosive features identified in Inca City, among them spiders, result from the seasonal melting of aqueous salty solutions.     

The influence of microgravity on the growth of metals and alloys—The project of Czechoslovak experiment

The crystal growth under near-zero gravity conditions may lead to materials of better crystalline and compositional perfection [1]. Unidirectional solidification of metals is a part of Czechoslovak programme on space research within the framework of Interkosmos [2]. On the model-like systems of metals grown in the space we want to study the effect of foreign atoms on the surface tension and the lattice defects density. The objectives of our ground-based and space experiments are discussed.     

Two-dimensional analyzer with dE-dE silicon detectors for cosmic ray experiments in space

A charged particle semiconductor-telescope is under construction in the CRIP, Budapest, to measure fluxes of 4–30 MeV/AMU protons, alphas, and M nuclei in interplanetary space. Identification and counting of particles are made on-board using microprocessor techniques. Details including some new ideas on economical coding of information and design of electronics are given.     

Annealing of Hg2Cl2 crystals — Project of the experiment

The creep of polycrystalline Hg₂X₂ (where X = Cl, Br, J) under the terrestrial and space conditions is studied in the present experiment. The authors suppose that the creep of the Hg₂X₂ cylindrical sample can be studied by the help of the theory of micropolar (non-Newtonian) fluids. In accordance with this theory we obtain the flux of fluid through any cross-section as functions of the radius, viscosity coefficients, the gradient of pressure and the gravity acceleration. In this paper a comparison of the theoretical results for normal and zero- gravity conditions is given. It is shown that the annealing of materials having a broad region of plasticity under microgravity conditions can lead to great improvement in optical as well as mechanical qualities of the crystal. Technical aspects of the experiment are also described.