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71.
G2-chromosome aberrations induced by high-LET radiations.   总被引:1,自引:0,他引:1  
We report measurement of initial G2-chromatid breaks in normal human fibroblasts exposed to various types of high-LET particles. Exponentially growing AG 1522 cells were exposed to gamma rays or heavy ions. Chromosomes were prematurely condensed by calyculin A. Chromatid-type breaks and isochromatid-type breaks were scored separately. The dose response curves for the induction of total chromatid breaks (chromatid-type + isochromatid-type) and chromatid-type breaks were linear for each type of radiation. However, dose response curves for the induction of isochromatid-type breaks were linear for high-LET radiations and linear-quadratic for gamma rays. Relative biological effectiveness (RBE), calculated from total breaks, showed a LET dependent tendency with a peak at 55 keV/micrometer silicon (2.7) or 80 keV/micrometer carbon (2.7) and then decreased with LET (1.5 at 440 keV/micrometer). RBE for chromatid-type break peaked at 55 keV/micrometer (2.4) then decreased rapidly with LET. The RBE of 440 keV/micrometer iron particles was 0.7. The RBE calculated from induction of isochromatid-type breaks was much higher for high-LET radiations. It is concluded that the increased production of isochromatid-type breaks, induced by the densely ionizing track structure, is a signature of high-LET radiation exposure.  相似文献   
72.
Changed political objectives, straitened economic circumstances and an altered balance of capability and expertise in space endeavours have together produced more discussion of the need for international cooperation than ever before, but the meaning of the term has subtly shifted. Insisting on US ‘leadership’ is self-defeating; what is instead firmly desired by the USA's potential partners is a balanced relationship in which each nation makes an identifiable contribution and takes the lead in at least one of a package of projects. If this is going to happen, there must be an international forum or framework in which projects can be discussed and agreed. This in turn requires initiative at governmental level.  相似文献   
73.
Cytogenetic effects of energetic ions with shielding   总被引:1,自引:0,他引:1  
In order to understand the effects of shielding on the induction of biological damages by charged particles, we conducted experiments with accelerated protons (250 MeV) and iron particles (1 GeV/u). Human lymphocytes in vitro were exposed to particle beams through polyethylene with various thickness, and chromosomal aberrations were determined using FISH technique. Dose response curves for chromosome aberrations were obtained and compared for various particle types. Experimental results indicated that for a given absorbed dose at the cell, the effectiveness of protons and iron particles in the induction of chromosomal aberrations was not significantly altered by polyethylene with thickness up to 30-cm and 15-cm respectively. Comparing with gamma rays, charged particles were very effective in producing complex chromosomal damages, which may be an important mechanism in alterating functions in nondividing tissues, such as nervous systems.  相似文献   
74.
We analyzed DNA and proteins obtained from normal and transformed human mammary epithelial cells for studying the neoplastic transformation by high-LET irradiation in vitro. We also examined microsatellite instability in human mammary cells transformed to various stages of carcinogenesis, such as normal, growth variant and tumorigenic, using microsatellite marker D5S177 on the chromosome 5 and CY17 on the Chromosome 10. Microsatellite instabilities were detected in the tumorigenic stage. These results suggest that microsatellite instability may play a role in the progression of tumorigenecity. The cause of the genomic instability has been suggested as abnormalities of DNA-repair systems which may be due to one of the three reasons: 1) alterations of cell cycle regulating genes. 2) mutations in any of the DNA mismatch repair genes. 3) mutation in any of the DNA strand breaks repair genes. No abnormality of these genes and encoded proteins, however was found in the present studies. These studies thus suggest that the microsatellite instability is induced by an alternative mechanism.  相似文献   
75.
This paper analyses potential future collaborative space exploration architectures in terms of 1) the technical capabilities of contributing partners; and 2) the constraints imposed by internal and international politics. We find that when international partners are considered endogenously, the argument for a “flexible path” approach is weakened substantially. This is because international contributions can make “Moon first” economically feasible; and characteristics of proposed “flexible path” approaches may preclude international involvement because of the disproportionate risk that those contributions inherently bear. This could have serious implications for future collaborations. We also note that while there are multiple feasible collaborative architectures, there is currently substantial overlap among the potential niche contributions identified by the international partners.  相似文献   
76.
Cosmic-ray isotope observations from NASAs Advanced Composition Explorer (ACE) mission have been used to investigate the composition of cosmic-ray source material. Source abundances relative to 56Fe are reported for eleven isotopes of Ca, Fe, Co, and Ni, including the very rare isotopes 48Ca and 64Ni. Although the source abundances range over a factor 104, most of the ratios to 56Fe are consistent with solar-system values to within 20%. However, there are some notable differences, the most significant being an excess of (70±30)% relative to the solar system for the cosmic-ray source ratio 58Fe/56Fe. The possible association of such an excess with a contribution to the cosmic-ray source from Wolf–Rayet star ejecta is discussed.  相似文献   
77.
Confluent human fibroblast cells were exposed to 6 Gy gamma-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy gamma-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track.  相似文献   
78.
79.
Pickup ions measured deep inside the heliosphere open a new way to determine the absolute atomic density of a number of elements and isotopes in the local interstellar cloud (LIC). We derive the atomic abundance of hydrogen and the two isotopes of helium from the velocity and spatial distributions of interstellar pickup protons and ionized helium measured with the Solar Wind Ion Composition Spectrometer (SWICS) on the Ulysses spacecraft between 2 and 5 AU. The atomic hydrogen density near the termination shock derived from interstellar pickup ion measurements is 0.115±0.025 cm–3 and the atomic H/He ratio from these observations is found to be 7.7 ± 1.3 in the outer heliosphere. Comparing this value with the standard universal H/He ratio of 10 we conclude that filtration of hydrogen is small and that the ionization fraction of hydrogen in the LIC is low.  相似文献   
80.
An Improved Piezoelectric Balance for Aerodynamic Force   总被引:1,自引:0,他引:1  
A method for measuring model forces in the shock tunnel by means of an acceleration-compensated balance has been reported by the authors.1 Since that time, a continuing program of research and development on advanced shock-tunnel instrumentation at Cornell Aeronautical Laboratory, Inc. has led to a promising new balance configuration. This balance is inherently more compact than previous designs, and allows testing of higher lift/drag (L/D) configurations than before. The increased high-pressure capability of the shock tunnel, and the growing recognition of the importance of the shock tunnel as a means of generating large Reynolds number flow, have thus added to the importance of this new higher load, more compact balance concept. An experimental balance has been built and calibrated. The performance of this balance is presented in this paper.  相似文献   
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