Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

Design of the TRIO system-on-chip for aerospace

Several design and testing aspects of the TRIO smart sensor data acquisition chip, developed by JHU/APL for NASA spacecraft applications are presented. TRIO includes a 10 bit self-corrected analog-to-digital converter (ADC), 16/32 analog inputs, a front end multiplexer with selectable aquisition time, a current source, memory, serial and parallel bus, and control logic. So far TRIO is used in many missions including Contour, Messenger, Stereo, Pluto, and the generic JPL X2000 spacecraft bus.     

Raman imagery: a new approach to assess the geochemical maturity and biogenicity of permineralized precambrian fossils

Laser-Raman imagery is a non-intrusive, non-destructive analytical technique, recently introduced to Precambrian paleobiology, that can be used to demonstrate a one-to-one spatial correlation between the optically discernible morphology and kerogenous composition of permineralized fossil microorganisms. Made possible by the submicron-scale resolution of the technique and its high sensitivity to the Raman signal of carbonaceous matter, such analyses can be used to determine the chemical-structural characteristics of organic-walled microfossils and associated sapropelic carbonaceous matter in acid-resistant residues and petrographic thin sections. Here we use this technique to analyze kerogenous microscopic fossils and associated carbonaceous sapropel permineralized in 22 unmetamorphosed or little-metamorphosed fine-grained chert units ranging from approximately 400 to approximately 2,100 Ma old. The lineshapes of the Raman spectra acquired vary systematically with five indices of organic geochemical maturation: (1) the mineral-based metamorphic grade of the fossil-bearing units; (2) the fidelity of preservation of the fossils studied; (3) the color of the organic matter analyzed; and both the (4) H/C and (5) N/C ratios measured in particulate kerogens isolated from bulk samples of the fossil-bearing cherts. Deconvolution of relevant spectra shows that those of relatively well-preserved permineralized kerogens analyzed in situ exhibit a distinctive set of Raman bands that are identifiable also in hydrated organic-walled microfossils and particulate carbonaceous matter freed from the cherts by acid maceration. These distinctive Raman bands, however, become indeterminate upon dehydration of such specimens. To compare quantitatively the variations observed among the spectra measured, we introduce the Raman Index of Preservation, an approximate measure of the geochemical maturity of the kerogens studied that is consistent both with the five indices of organic geochemical alteration and with spectra acquired from fossils experimentally heated under controlled laboratory conditions. The results reported provide new insight into the chemical-structural characteristics of ancient carbonaceous matter, the physicochemical changes that accompany organic geochemical maturation, and a new criterion to be added to the suite of evidence by which to evaluate the origin of minute fossil-like objects of possible but uncertain biogenicity.     

Estimating the spin axis orientation of the Echostar-2 box-wing geosynchronous satellite

For the first time, the spin axis orientation of an inactive box-wing geosynchronous satellite has been estimated from ground-based optical photometric observations of Echostar-2’s specular reflections. Recent photometric light curves obtained of Echostar-2 over four years suggest that unusually bright and brief specular reflections were occurring twice within an observed spin period. These bright and brief specular reflections suggested two satellite surfaces with surface normals separated by approximately 180°. The geometry between the satellite, the Sun, and the observing location at the time of each of the brightest observed reflections, was used to estimate Echostar-2’s equatorial spin axis orientation coordinates. When considering prograde and retrograde rotation, Echostar-2’s spin axis orientation was estimated to have been located within 30° of either equatorial coordinate pole. Echostar-2’s spin axis was observed to have moved approximately 180° in right ascension, within a time span of six months, suggesting a roughly one year spin axis precession period about the satellite’s angular momentum vector.     

MMS星座对磁层顶磁通量绳内离子惯性尺度结构的观测

利用MMS观测数据,对磁层顶通量绳内离子惯性尺度（d_i）的结构进行分析研究.结果发现,许多不同尺度（约1d_i至数十d_i）的通量绳内都存在具有d_i尺度的电流 j _m,其方向在磁层顶局地坐标系的-M方向,即与磁层顶查普曼-费拉罗电流同向,由电子在+M方向的运动（ v _em）携带.这些电流结构具有以下特征：磁鞘与磁层成分混合,磁场为开放形态;离子去磁化,电子与磁场冻结;N方向（即垂直于磁层顶电流片方向）的电场 E _n显著增大,幅度达到约20mV·m^-1,并伴有明显的尖峰状起伏,该增强和尖峰状起伏的电场对应于霍尔电场.分析表明,电流、电子与离子运动的偏离以及霍尔电场之间遵从广义欧姆定律,三者密切关联.进一步对磁层顶磁重联的探测数据进行分析发现,在很多重联区内也存在与通量绳内相似的结构,其尺度约为d_i量级,其中霍尔电场 E _N、电流 j _M和电子速度 v _eM均与通量绳内对应物理量的方向相同且幅度相近.基于上述观测事实,采用经典FTE通量绳模型,对通量绳内电流、电子运动和霍尔电场的起源进行了初步探讨,认为其来源于磁层顶无碰撞磁重联区内的相应结构,并且后者在离子尺度通量绳的形成过程中起到重要作用.     

Composite model of a research flight simulator for a helicopter with the hingeless main rotor

This paper presents a composite model of a flight simulator. This simulator is intended for carrying out investigative tests of flight dynamics imitation models of a hingeless rotor helicopter. A schematic diagram, composition and operation channels of the flight simulator software are presented. A possibility of applying the control lag is estimated.     

The Extreme Ultraviolet Imager Investigation for the IMAGE Mission

The Extreme Ultraviolet Imager (EUV) of the IMAGE Mission will study the distribution of He⁺ in Earth's plasmasphere by detecting its resonantly-scattered emission at 30.4 nm. It will record the structure and dynamics of the cold plasma in Earth's plasmasphere on a global scale. The 30.4-nm feature is relatively easy to measure because it is the brightest ion emission from the plasmasphere, it is spectrally isolated, and the background at that wavelength is negligible. Measurements are easy to interpret because the plasmaspheric He⁺ emission is optically thin, so its brightness is directly proportional to the He⁺ column abundance. Effective imaging of the plasmaspheric He⁺ requires global `snapshots in which the high apogee and the wide field of view of EUV provide in a single exposure a map of the entire plasmasphere. EUV consists of three identical sensor heads, each having a field of view 30° in diameter. These sensors are tilted relative to one another to cover a fan-shaped field of 84°×30°, which is swept across the plasmasphere by the spin of the satellite. EUVs spatial resolution is 0.6° or 0.1 R _E in the equatorial plane seen from apogee. The sensitivity is 1.9 count s^–1 Rayleigh^–1, sufficient to map the position of the plasmapause with a time resolution of 10 min.     

Surface characteristics of spacecraft components affect the aggregation of microorganisms and may lead to different survival rates of bacteria on Mars landers

Layers of dormant endospores of Bacillus subtilis HA101 were applied to eight different spacecraft materials and exposed to martian conditions of low pressure (8.5 mbar), low temperature (-10 degrees C), and high CO(2) gas composition and irradiated with a Mars-normal ultraviolet (UV-visible- near-infrared spectrum. Bacterial layers were exposed to either 1 min or 1 h of Mars-normal UV irradiation, which simulated clear-sky conditions on equatorial Mars (0.1 tau). When exposed to 1 min of Mars UV irradiation, the numbers of viable endospores of B. subtilis were reduced three to four orders of magnitude for two brands of aluminum (Al), stainless steel, chemfilm-treated Al, clear-anodized Al, and black-anodized Al coupons. In contrast, bacterial survival was reduced only one to two orders of magnitude for endospores on the non-metal materials astroquartz and graphite composite when bacterial endospores were exposed to 1 min of Mars UV irradiation. When bacterial monolayers were exposed to 1 h of Mars UV irradiation, no viable bacteria were recovered from the six metal coupons listed above. In contrast, bacterial survival was reduced only two to three orders of magnitude for spore layers on astroquartz and graphite composite exposed to 1 h of Mars UV irradiation. Scanning electron microscopy images of the bacterial monolayers on all eight spacecraft materials revealed that endospores of B. subtilis formed large aggregates of multilayered spores on astroquartz and graphite composite, but not on the other six spacecraft materials. It is likely that the formation of multilayered aggregates of endospores on astroquartz and graphite composite is responsible for the enhanced survival of bacterial cells on these materials.     

The Rosat mission

The primary scientific objective of the ROSAT mission is to perform the first all sky survey with an imaging X-ray telescope leading to an improvement in sensitivity by several orders of magnitude compared with previous surveys. Consequently a large number of new sources (> 10⁵) will be discovered and located with an accuracy of 1 arcmin. After completion of the survey which will take about half a year the instrument will be used for detailed observations of selected targets.The X-ray telescope consists of a fourfold nested Wolter type I mirror system with 80 cm aperture and 240 cm focal length, and three focal plane detectors. In the baseline version these will be imaging proportional counters (0.1 – 2 keV) providing a field of view of 2⁰ × 2⁰.     

Space–Time Structure of Energetic Electron Precipitations according to the Data of Balloon Observations and Polar Satellite Measurements on February 1–6, 2015

Cosmic Research - The results of an analysis of the space–time characteristics and dynamics of precipitations of magnetospheric electrons with energies in the range from 0.1 to 0.7 MeV are...