Reduced lymphocyte activation in space: role of cell-substratum interactions.

We investigated the effect of substratum adhesiveness on stimulated lymphocyte blastogenesis by reducing and blocking cell adhesion with poly (2-hydroxyethyl methacrylate) (poly-HEMA) in a simple on-ground system. Cells grown on medium-thick and thick poly-HEMA films were rounded in shape and displayed no signs of spreading. By contrast, on tissue culture plastic and very thin poly-HEMA films, they showed clear signs of spreading. The mitogenic response of lymphocytes grown on thick poly-HEMA films was reduced by up to 68% of the control (tissue culture plastic). Interferon-gamma production was near zero when the cells were grown on the least adhesive substratum. On uncoated plastic, activated lymphocytes subjected to high gravity (20g) exhibited an increased proliferation rate (40%) compared with 1g. By contrast, on poly-HEMA, high gravity did not improve lymphocyte responsiveness. These results show that activated lymphocytes need to anchor and spread prior to achieving an optimal proliferation response. We conclude that decreased lymphocyte adhesion could contribute to the depressed in vitro lymphocyte responsiveness found in the microgravity conditions of space flight.     

The γ-ray bursts as a result of the proper activity of the neutron star

The model of the Γ-ray burst is developed which is based on the nuclear explosion in the neutron star envelope because of the chain fission reaction of the superheavy nuclei. It is shown, that the main part of the Γ-ray burst radiation must have thermal origin, but the nonthermal component and Γ-ray lines may be present. The parameters of the strong burst of 5 March 1979 are evaluated on the basis of its radiation spectra and the present model. The distance to this burst is about 100 pc and the total energy of this Γ-ray burst is about 2.1039 ergs.     

Development of x-ray mirrors for high-energy astrophysics in czechoslovakia

The grazing incidence X-ray mirrors with diameters between 20 and 240 mm were produced by two different replica techniques. Five mirrors were flown in space experiments. It has been confirmed that the galvanoplastic replica mirrors are well suited for applications in space telescopes and laboratory microscopes.     

Instrumentenentwicklungen für anwendungsaufgaben der fernerkundung photogrammetrie,geophysik und geodäsie

(Instrument Developments for Applications in Remote Sensing, Photogrammetry, Geophysics and Geodesy) The Modular Optoelectronic Multispectral Seanner MOMS-01, a CCD camera using the push-broom scanner principle, is presented in this paper in its flight configuration. This instrument will be the first European-built spaceborne unaging system for remote sensing applications (launch with Shuttle flight No. 7).Follow-on developments of this initial two-channel version are oriented to an extension of capabilities by a panchromatic high-resolution stereoscopic module for thematic mapping and two additional spectral channels in the reflective IR (up to 2.3 μm) offering a high interpretation potential for future earth resources exploration missions. Furthermore the subjects of advanced laser technology studies are introduced: A picosecond pulse laser system for detection of tectonic motions and for geodetic application, a CO laser for ultra precise range rate determination (Earth potential and Geoid) and a laser range finder for intersatellite distance measurements.     

Cellular parameters for track structure modeling of radiation hazard in space.

Based on irradiation with 45 MeV/u N and B ions and with Co-60 gamma rays, cellular parameters of Katz's track structure model have been fitted for the survival of V79-379A Chinese hamster lung fibroblasts. Cellular parameters representing neoplastic transformations in C3H10T/1/2 cells after their irradiation with heavy ion beams, taken from earlier work, were also used to model the radiation hazard in deep space, following the system for evaluating, summing and reporting occupational exposures proposed in 1967 by a subcommittee of NCRP. We have performed model calculations of the number of transformations in surviving cells, after a given fluence of heavy charged particles of initial energy 500 MeV/u, penetrating thick layers of cells. We take the product of cell transformation and survival probabilities, calculated along the path lengths of charged particles using cellular survival and transformation parameters, to represent a quantity proportional to the "radiation risk factor" discussed in the NCRP document. The "synergistic" effect of simultaneous charged particle transfers is accounted for by the "track overlap" mode inherent in the model of Katz.     

Uncertainties in estimates of the risks of late effects from space radiation.

Methods used to project risks in low-Earth orbit are of questionable merit for exploration missions because of the limited radiobiology data and knowledge of galactic cosmic ray (GCR) heavy ions, which causes estimates of the risk of late effects to be highly uncertain. Risk projections involve a product of many biological and physical factors, each of which has a differential range of uncertainty due to lack of data and knowledge. Using the linear-additivity model for radiation risks, we use Monte-Carlo sampling from subjective uncertainty distributions in each factor to obtain an estimate of the overall uncertainty in risk projections. The resulting methodology is applied to several human space exploration mission scenarios including a deep space outpost and Mars missions of duration of 360, 660, and 1000 days. The major results are the quantification of the uncertainties in current risk estimates, the identification of factors that dominate risk projection uncertainties, and the development of a method to quantify candidate approaches to reduce uncertainties or mitigate risks. The large uncertainties in GCR risk projections lead to probability distributions of risk that mask any potential risk reduction using the "optimization" of shielding materials or configurations. In contrast, the design of shielding optimization approaches for solar particle events and trapped protons can be made at this time and promising technologies can be shown to have merit using our approach. The methods used also make it possible to express risk management objectives in terms of quantitative metrics, e.g., the number of days in space without exceeding a given risk level within well-defined confidence limits.     

Daytime VHF amplitude scintillations recorded at an Indian low-latitude station,Waltair (17.7°N, 83.3°E) during 1997–2003

In this research, it is presented the daytime amplitude scintillations recorded at VHF frequency (244 MHz) at an Indian low-latitude station, Waltair (17.7°N, 83.3°E) during seven continuous years (1997–2003). Contrary to the nighttime scintillation seasonal trends, the occurrence of daytime scintillations maximizes during summer followed by winter and the equinox seasons. The fade depths, scintillation indices and the patch durations of daytime scintillations are meager when compared with their nighttime counterparts. A co-located digital high frequency (HF) ionosonde radar confirms the presence of sporadic (Es) layers when daytime scintillations are observed. The presence of daytime scintillations is evident when the critical frequency of the Es-layer (foEs) is ≥4 MHz and Es-layers are characterized by a highly diffuse range spread Es echoes as can be seen on ionograms. It is surmised that the gradient drift instability (GDI) seems to be the possible mechanism for the generation of these daytime scintillations. It is quite likely that the spread Es-F-layer coupling is done through polarization electric fields (Ep) that develop inside the destabilized patches of sporadic E layers, which are mapped up to the F region along the field lines as to initiate the daytime scintillations through the GDI mechanism. Further, the presence of additional stratification of ionosphere F-layer, popularly known as the F3-layer, is observed on ionograms once the Es-layers and daytime scintillations are ceased.     

Exploring the Detached-Eddy Simulation for Main Rotor Flows

This paper applies the Detached-Eddy Simulation (DES) method to resolve a larger part of the flow spectrum around rotor blades in hover and forward flight. A comparison between DES and Unsteady Reynolds–Averaged Navier–Stokes simulation was carried out for the case of a forward flying rotor suggesting that DES has great potential for rotor applications.